Airborne transmission-mediated delivery of a live-attenuated influenza virus vector-based intranasal COVID-19 vaccine protects Syrian hamsters against SARS-CoV-2 challenge

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Author notes

1. These authors contributed equally: Yaode Chen, Limin Zhang, Congjie Chen, Lunzhi Yuan.

Authors and Affiliations

1. State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, China

   Yaode Chen, Limin Zhang, Congjie Chen, Lunzhi Yuan, Qiangyuan Han, Xijing Wang, Heming Chen, Xiangjie Chen, Ruotong Wu, Zeheng Li, Lingyu Han, Zhen Lu, Dongmei Wei, Ningshao Xia, Junyu Chen & Yixin Chen

2. National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, National Innovation Platform for Industry-Education Integration in Vaccine Research, Xiamen University, Xiamen, China

   Limin Zhang, Lunzhi Yuan, Ningshao Xia, Junyu Chen & Yixin Chen

Contributions

J.C., N.X., and Y.C. conceived the project and designed the experiments; Y.C., L.Z., C.C., L.H., Z.L., and D.W. prepared the dNS1-RBD vaccines, performed viral characterization experiments; Y.C., L.Z., C.C., L.Y., X.W., R.W., Z.L., and Q.H. participated in the mice, ferrets and hamsters challenges; L.Y., H.C., and X.C. tested the RNA quantification and histopathology; Y.C., L.Z., and J.C analyzed data; Y.C., L.Z., J.C., and Y.C. wrote the manuscript; Y.C., L.Z., N.X., J.C., and Y.C. participated in the discussion and interpretation of the results. All authors reviewed and approved the paper. AI-assisted technologies were used in the writing process for revision and modification.

Corresponding authors

Correspondence to Junyu Chen or Yixin Chen.

Competing interests

The authors declare no competing interests.

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