Biological evaluation of chelated trace minerals with multi-strain probiotics and enzymes on production performance of broiler chickens

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Biological evaluation of chelated trace minerals with multi-strain probiotics and enzymes on production performance of broiler chickens

References

  1. OECD-FAO Agricultural Outlook 2023–2032. Available online at (2024). https://www.oecd.org/publications

  2. Choi, J., Kong, B., Bowker, B. C., Zhuang, H. & Kim, W. K. Nutritional strategies to improve meat quality and composition in the challenging conditions of broiler production: a review. Anim 13, 1386 (2023).

    Google Scholar 

  3. Biabani, N. et al. Dietary advanced chelate technology-based 7-mineral supplement improves growth performance and intestinal health indicators during a mixed Eimeria challenge in broiler chickens. Vet. Parasitol. 331, 110277 (2024).

    Google Scholar 

  4. Soni, N. et al. Bioavailability and immunity response in broiler breeders on organically complexed zinc supplementation. Food Nutri Sci. 4, 1293–1300 (2013).

    Google Scholar 

  5. Ayalew, H. et al. Potential feed additives as antibiotic alternatives in broiler production. Front. Vet. Sci. 9, 916–473 (2022).

    Google Scholar 

  6. Yaqoob, M. U., Wang, G. & Wang, M. An updated review on probiotics as an alternative of antibiotics in poultry – A review. Anim. Biosci. 35, 1109–1120 (2022).

    Google Scholar 

  7. Gao, P. et al. Feed-additive probiotics accelerate yet antibiotics delay intestinal microbiota maturation in broiler chicken. Micro 5, 1–14 (2017).

    Google Scholar 

  8. Alshelmani, M. I., El-Safty, S. A., Kairalla, M. A. & Humam, A. M. Enzymes in poultry feed. In: L. Babinszky (ed.), Feed additives – recent trends in animal nutrition. IntechOpen London, UK. (2024).

  9. Official methods of analysis of AOAC International. Association of Official Analytical Chemists 17th edn (Gaithersburg, 2000).

  10. Zhao, Z. et al. Effects of a novel synbiotics-enzyme complex as a replacement for antibiotics on growth performance, slaughter and meat characteristics, immune organ index and intestinal morphology of broilers. Front. Vet. Sci. 11, 1468–1847 (2024).

    Google Scholar 

  11. Mohamme, A. A., Zaki, R. S., Negm, E. A., Mahmoud, M. A. & Chengk, H. W. Effects of dietary supplementation of a probiotic (Bacillus subtilis) on bone mass and meat quality of broiler chickens. Poult. Sci. 100, 100–906 (2020).

    Google Scholar 

  12. Patterson, P. H., Cook, M. E., Crenshaw, T. D. & Sunde, M. L. Mechanical properties of the tibiatarsus of broilers and Poults loaded with artificial weight and fed various dietary protein levels. Poult. Sci. 65, 1357–1364 (1986).

    Google Scholar 

  13. Seedor, J. G., Quarruccio, H. A. & Thompson, D. D. The bisphosphonate alendronate (MK-217) inhibits bone loss due to ovariectomy in rats. J. Bone Min. Res. 6, 339–346 (1991).

    Google Scholar 

  14. Barnet, E. & Nordin, B. The radiological diagnosis of osteoporosis: A new approach. Clin. Radiol. 11, 166–169 (1960).

    Google Scholar 

  15. Steel, R. G. D., James, H. T. & David, A. D. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edition. McGraw-Hill Book Company, New York, United States of America. (1997).

  16. Kiarie, E., Romero, L. & Ravindran, V. Growth performance, nutrient utilizationand digesta characteristics in broiler chickens fed corn or wheat diets without or with supplemental Xylanase. Poul Sci. 93, 1186–1193 (2014).

    Google Scholar 

  17. Biswas, S., Kim, M. H., Dong, H. B. & Kim, I. H. Probiotic mixture (Bacillus subtilis and Bacillus licheniformis): A potential in-feed additive to improve broiler production efficiency, nutrient digestibility, caecal microflora, meat quality and to diminish hazardous odour emission. J. Anim. Physiol. Anim. Nutri. 107, 1065–1072 (2023).

    Google Scholar 

  18. Nadeem, M., Anjum, M., Khan, A. & Azim, A. Effect of dietary supplementation of non-starch polysaccharide degrading enzymes on growth performance of broiler chicks. Pak Vet. J. 25, 183–188 (2005).

    Google Scholar 

  19. Yaseen, M. A. et al. Dietary supplementation of protease and organic acid in poultry by-product meal-based diet in broilers. Anim. Bio. 37, 2145–2154 (2024).

    Google Scholar 

  20. Vaarst, M., Steenfeldt, S. & Horsted, K. Sustainable development perspectives of poultry production. World Poul Sci. J. 71, 609–620 (2015).

    Google Scholar 

  21. Manafi, M., Khalaji, S., Hedayati, M. & Pirany, N. Efficacy of Bacillus subtilis and bacitracin methylene disalicylate on growth performance, digestibility, blood metabolites, immunity, and intestinal microbiota after intramuscular inoculation with Escherichia coli in broilers. Poul sci. pp. 96 (5), 1174–1183 (2017).

    Google Scholar 

  22. Zamany, S., Sedghi, M., Hafizi, M., Lotfollahian, H. & Hasanpur, K. Organic acid-based chelate trace mineral supplement improves broiler performance, bone composition, immune responses and blood parameters. Bio Tra Elem. Res. 201, 4882–4899 (2023).

    Google Scholar 

  23. Ciurescu, G., Vasilachi, A. & Grosu, H. Efficacy of microbial phytase on growth performance, carcass traits, bone mineralization, and blood biochemistry parameters in broiler Turkeys fed Raw Chickpea (Cicer arietinum L., cv. Burnas) diets. J. App Poult. Res. pp 29 (1), 171–184 (2020).

    Google Scholar 

  24. Leeson, S. & Summers, J. D. Nutrition of the Chicken (University Books, 2001).

  25. Vimon, S., Angkanaporn, K. & Nuengjamnong, C. Microencapsulation of Lactobacillus plantarum MB001 and its probiotic effect on growth performance, cecal Microbiome and gut integrity of broiler chickens in a tropical climate. Anim. biosci. 36 (8), 12–52 (2023).

    Google Scholar 

  26. Neveling, D. P. & Dick, L. M. Probiotics: an antibiotic replacement strategy for healthy broilers and productive rearing. Prob Anti Prot. 13 (1), 1–11 (2021).

    Google Scholar 

  27. Mallo, J. J. et al. Effect of a mixed-enzyme product on broiler metabolizable energy, phosphorus and calcium retention. J. Anim. Sci. 51, 246–257 (2025).

    Google Scholar 

  28. Sugiharto, S., Adli, D. N., Ujilestar, T., Sholikin, M. M. & Inggrida, J. A. Does the use of Whey protein in broiler chicken diets have a positive effect? Model-based meta-analysis. J. Agri Sci. 163 (4), 403–425 (2025).

    Google Scholar 

  29. Nollet, L., Van Der Klis, L., Lensing, M. & Spring, P. The effect of replacing inorganic with organic trace minerals in broiler diets on productive performance and mineral excretion. J. App Poult. Res. 16, 592–597 (2007).

    Google Scholar 

  30. Zaghari, M., Sarani, P. & Hajati, H. Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens. J. App Anim. Res. 48, 166–175 (2020).

    Google Scholar 

  31. Yaqoob, M. U. et al. Effect of multi-enzymes supplementation on growth performance, meat quality, ileal digestibility, digestive enzyme activity and caecal microbiota in broilers fed low-metabolizable energy diet. Anim. Biosci. 35, 1059–1068 (2022).

    Google Scholar 

  32. Dimas, A. T. et al. Effect of Corn-Expressed glucanase on mineral digestibility and apparent metabolizable energy in broiler diets. Inter J. Poult. Sci. 22, 1216 (2023).

    Google Scholar 

  33. Kim, M. et al. Synergistic effect of exogenous multi-enzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora and morphology in broilers fed corn-wheat-soybean meal diets. Anim. Biosci. 34, 1365–1374 (2021).

    Google Scholar 

  34. Hashemi, R., Palizdar, M. H., Tabrizi, H. M. & Rostami, M. Varied sources and levels of zinc in Ross 308 broiler rations: its effect on performance, nutrient utilization, intestinal morphology and microbial composition. Arq. Brasil De Medi Vet. E Zoot. 76, 346–356 (2024).

    Google Scholar 

  35. Luo, C., Wang, L., Chen, Y. & Yuan, J. Supplemental enzyme and probiotics on the growth performance and nutrient digestibility of broilers fed with a newly harvested corn diet. Anim 12, 2381 (2022).

    Google Scholar 

  36. Rodriguez-Soriano, F. A. et al. Sfericase protease, phytase and Xylanase combination improves body weight, feed conversion rate, ileal digestibility and gut morphology in broilers. Front. Anim. Sci. 6, e1453735 (2025).

    Google Scholar 

  37. Khan, A. et al. Optimising growth performance, nutrients digestibility, immunity and gut health in broilers through ginger-derived phyto-protease enzyme (zingibain) supplementation. Ital. J. Anim. Sci. 23, 1695–1703 (2024).

    Google Scholar 

  38. Leeson, S. A new look at trace mineral nutrition of poultry: Can we reduce environmental burden of poultry manure? Proceedings of the Alltech’s 19th Annual Symposium, Lexington, KY. 125-129. (2003).

  39. Timmerman, H. M., Koning, C. J., Mulder, L., Rombouts, F. M. & Beynen, A. C. Monostrain, multistrain and multispecies probiotics—A comparison of functionality and efficacy. Inter J. Food Micro. 96 (3), 219–233 (2004).

    Google Scholar 

  40. Qiu, K. et al. Dietary supplementation of a new probiotic compound improves the growth performance and health of broilers by altering the composition of cecal microflora. Biol 11 (5), 633 (2022).

    Google Scholar 

  41. El-Kelawy, M. Immunophysiological and productive response of broiler chicks to dietary supplementation with multi-enzyme and/or probiotics. Egyp Poult. Sci. 38, 1047–1067 (2018).

    Google Scholar 

  42. Attia, Y. A., Al-Khalaifah, H., El-Hamid, H. S. A., Al-Harthi, H. A. S. & El-Shafey, A. A. Effect of different levels of multienzymes on immune response, blood hematology and biochemistry, antioxidants status and organs histology of broiler chicks fed standard and low-density diets. Front. Vet. Sci. 6, 510–524 (2020).

    Google Scholar 

  43. Zhyla, M. et al. Blood indices and immune organs morphological structure of broiler chickens under the influence of different doses of probiotic feed additives. Ukrai J. Vet. Agri Sci. 5, 17–27 (2022).

    Google Scholar 

  44. Sjofjan, O. et al. The effects of lactic acid bacteria and yeasts as probiotics on the growth performance, relative organ weight, blood parameters and immune responses of broiler: A meta-analysis. F1000Res 10, 183 (2021).

    Google Scholar 

  45. Hashemitabar, S. H. & Hosseinian, S. A. The comparative effects of probiotics on growth, antioxidant indices and intestinal histomorphology of broilers under heat stress condition. Sci. Rep. 14, 23471 (2024).

    Google Scholar 

  46. Hossain, H., Nuradji, H., Miah, M. Y., Islam, M. N. & Siddiqui, M. S. I. Impact of synbiotic on growth performance, histo-architectural modulation of lymphoid organ, hematology, blood biochemistry and humoral immune response in naked neck chicken. Trop. Anim. Hea Prod. 57, 4 (2024).

    Google Scholar 

  47. Dibamehr, A., Daneshyar, M., Tukmechi, A. & Abtahi Froushani, S. M. Effects of two plant extracts and native Lactobacillus culture on immune response, lymphoid organs and antioxidant properties of broiler chickens. J. Cent. Europ Agri. 24, 601–612 (2023).

    Google Scholar 

  48. Mangisah, I., Yunianto, V. D., Sumarsih, S. & Sugiharto, S. Effect of Lactobacillus casei and Garlic powder administration on broiler performance, immune response and blood profile. Indo J. Anim. Vet. Sci. 27, 152–158 (2022).

    Google Scholar 

  49. Nunez, R., Elliott, S. & Riboty, R. The effect of dietary supplementation of organic trace minerals on performance, mineral retention, lymphoid organs and antibody titres of broilers. J. Appl. Anim. Nut. 11, 23–32 (2022).

    Google Scholar 

  50. Islam, M. S., Rahman, M. M., Islam, M. S. D., Khalil, K. K. I. & Mondal, A. K. Appraisal of suitability of probiotics over antibiotic growth promoter supplementation on growth performances and hematology in broilers. Vet. Sci. Res. Rev. 10, 1521 (2025).

    Google Scholar 

  51. Younas, S., Bukhari, D. A., Bibi, Z., Ullah, A. & Rehman, A. Impact of multistrain probiotics on growth performance, immune response and gut morphometry in broiler chicken Gallus Gallus domesticus. Poult. Sci. 104, 105026 (2025).

    Google Scholar 

  52. El-Sayed, Y., Khalil, W., Fayez, N. & Abdel-Fattah, A. F. M. Enhancing effect of oregano essential oil and Bacillus subtilis on broiler immune function, intestinal morphology and growth performance. BMC Vet. Res. 20, 112 (2024).

    Google Scholar 

  53. Hidayat, M. N., Malaka, R., Agustina, L. & Pakiding, W. Effect of probiotic Lactobacillus paracasei on hematology and relative weight of lymphoid organs of broiler. In Proceedings of the 2nd Inter Conf Anim Sci Tech (ICAST 2019), Mak Indo. 492:e012127. (2020).

  54. Ayoola, A. A., Fafiolu, A. O., Oluwatosin, O. O., Osinowo, O. A. & Ariyo, O. W. Haematological and serum biochemical indices of broiler chickens fed graded levels of inorganic and chelated trace minerals. Nig J. Anim. Prod. 47, 46–50 (2020).

    Google Scholar 

  55. Mahmoud, A., Khader, N. & Hegazy, A. Dose dependent effect of phytase supplementation on hematological parameters, serum biochemical analysis, carcass characteristics and chemical meat analysis of Hubbard broiler chickens. Benh Vet. Med. J. 47, 1218 (2024).

    Google Scholar 

  56. Abdilahi, M. Y. Effects of different levels of multi-enzymes (Polzyme® liquid) on the growth performance, carcass traits, and meat quality of broiler chickens. J. Ist Vet. Sci. 9, 19 (2025).

    Google Scholar 

  57. Bao, Y. M., Choct, M., Iji, P. A. & Bruerton, K. Effect of organically complexed copper, iron, manganese and zinc on broiler performance, mineral excretion and accumulation in tissues. J. Appl. Poult. Res. 16, 448–455 (2007).

    Google Scholar 

  58. Mohammadizad, T. et al. Potential benefits of advanced chelate-based trace minerals in improving bone mineralization, antioxidant status, immunity and gene expression modulation in heat-stressed broilers. PLoS ONE. 19, e0311083 (2024).

    Google Scholar 

  59. Sohail, S., Mottaghitalab, M., Hossein-Zadeh, N. G. & Nazaran, M. H. Effect of advanced chelate technology-based trace minerals on growth performance, mineral digestibility, tibia properties and antioxidant status in two broiler strains. Poul Sci. 103, 104304 (2024).

    Google Scholar 

  60. Lee, Y. S., Ku, K. L., Chu, C. S. & Chen, K. L. The optimal supplementation of fermented product produced by Bacillus subtilis strain LYS1 with high surfactin yield for improving growth performance, intestinal villi morphology and tibial bone strength in broilers. Animals 14: (2079).

  61. Guo, S. et al. Interactive effects of dietary vitamin K3 and Bacillus subtilis PB6 on the growth performance and tibia quality of broiler chickens with sex separate rearing. Anim. 2024.14:1610–1618. (2020).

  62. Sahraei, M., Janmmohamdi, H., Taghizadeh, A. & Cheraghi, S. Effect of different zinc sources on tibia bone morphology and Ash content of broiler chickens. Adv. Biol. Res. 6, 128–132 (2012).

    Google Scholar 

  63. Shah, M. et al. Effect of single or combined supplementation of zinc and probiotics on muscle and bone characteristics and haematobiochemical profile in broilers. Vet. Med. 65, 134–142 (2020).

    Google Scholar 

  64. Steczny, K. & Kokoszynski, D. Effect of probiotic preparations (EM) and sex on morphometric characteristics of the digestive system and leg bones caecal microflora in broiler chickens. J. Appl. Anim. Res. 48, 45–50 (2020).

    Google Scholar 

  65. Hajimohammadi, A., Mottaghitalab, M. & Hashemi, M. Effects of microbial fermented Sesame meal and enzyme supplementation on the intestinal morphology, microbiota, pH, tibia bone and blood parameters of broiler chicks. Ital. J. Anim. Sci. 19, 457–467 (2020).

    Google Scholar 

  66. Wang, Z. R., Qiao, S. Y., Lu, W. Q. & Li, D. F. Effects of enzyme supplementation on performance, nutrient digestibility, Gastrointestinal morphology and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets. Poult. Sci. 84, 875–881 (2005).

    Google Scholar 

  67. Liu, L. et al. Effects of Lactiplantibacillus plantarum lpjz-658 supplementation on the production, meat quality, intestinal morphology and cecal microbiota of broilers chickens. Microorg 11, 1549 (2023).

    Google Scholar 

  68. Tang, X., Liu, X. & Liu, H. Effects of dietary probiotic (Bacillus subtilis) supplementation on carcass traits, meat quality, amino acid and fatty acid profile of broiler chickens. Front. Vet. Sci. 8, 767802 (2021).

    Google Scholar 

  69. Yibar, A. & Uzabaci, E. Meta-analysis to predict the effects of probiotics on meat quality of broiler. J. Anim. Physiol. Anim. Nut. 108, 1616–1623 (2024).

    Google Scholar 

  70. Li, L. et al. Effect of manganese amino acid complexes on growth performance, meat quality, breast muscle and bone development in broilers. Brit Poult. Sci. 2024 65:582–594 .

  71. Zamani, H. U., Loh, T. C., Foo, H. L., Samsudin, A. A. & Alshelmani, M. I. Effects of feeding palm kernel cake with crude enzyme supplementation on growth performance and meat quality of broiler chicken. Inter J. Micro Biotech. 2, 22–28 (2017).

    Google Scholar 

  72. Suryadi, U., Nugraheni, Y. R., Prasetyo, A. F. & Awaludin, A. Evaluation of effects of a novel probiotic feed supplement on the quality of broiler meat. Vet. Worl. 12, 1775–1778 (2019).

    Google Scholar 

  73. Lu, P. et al. Effects of antibiotic growth promoter and dietary protease on growth performance, apparent ileal digestibility, intestinal morphology, meat quality and intestinal gene expression in broiler chickens: a comparison. J. Anim. Sci. 98, skaa254 (2020).

    Google Scholar 

  74. Harun-Or-Rashid, M. Effects of different levels of selenium supplements on growth performance and immune response of broiler chicken in hot humid climatic condition (Doctoral dissertation; Department of animal nutrition, genetics and breeding). (2020).

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