RSM and ANN-GA assisted optimization of Morchella importuna extracts and their effects on antioxidant anticholinesterase and antiproliferative activity

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RSM and ANN-GA assisted optimization of Morchella importuna extracts and their effects on antioxidant anticholinesterase and antiproliferative activity

References

  1. Fitsum, S., Sbhatu, D. B. & Gebreyohannes, G. Harnessing the nutritional Value, therapeutic Applications, and environmental impact of mushrooms. Food Sci. Nutr. 13, e70611 (2025).

    Google Scholar 

  2. El-Ramady, H. et al. Plant nutrition for human health: a pictorial review on plant bioactive compounds for sustainable agriculture. Sustainability 14, 8329 (2022).

    Google Scholar 

  3. Sevindik, M., Bal, C., Eraslan, E. C., Uysal, I. & Mohammed, F. S. Medicinal mushrooms: a comprehensive study on their antiviral potential. Prospects Pharm. Sci. 21, 42–56 (2023).

    Google Scholar 

  4. Gogoi, P., Chutia, P., Singh, P. & Mahanta, C. L. Effect of optimized ultrasound-assisted aqueous and ethanolic extraction of pleurotus citrinopileatus mushroom on total phenol, flavonoids and antioxidant properties. J. Food Process Eng. 42, e13172 (2019).

    Google Scholar 

  5. Gürgen, A. & Sevindik, M. Application of artificial neural network coupling multiobjective particle swarm optimization algorithm to optimize pleurotus ostreatus extraction parameters. J. Food Process. Preserv. 46, e16949 (2022).

    Google Scholar 

  6. Sambyal, K. & Singh, R. V. A comprehensive review on Morchella importuna: cultivation aspects, phytochemistry, and other significant applications. Folia Microbiol. 66, 147–157 (2021).

    Google Scholar 

  7. Kumar, A., Sharma, V. P. & Kumar, S. Studies on domestication of the true Morel Morchella importuna in India. Indian Phytopathol. 77, 301–310 (2024).

    Google Scholar 

  8. Zhang, Y. et al. Comprehensive investigation on non-volatile and volatile flavor compounds in the Morchella sextelata and Morchella importuna by UPLC-MS/MS and GC× GC-TOF-MS. Food Chemistry: X. 20, 100961 (2023).

    Google Scholar 

  9. Peng, D. et al. A new GlcNAc-containing polysaccharide from Morchella importuna fruiting bodies: structural characterization and Immunomodulatory activities in vitro and in vivo. Int. J. Biol. Macromol. 192, 1134–1149 (2021).

    Google Scholar 

  10. Wen, Y. et al. Structural characterization and Immunomodulatory mechanisms of two novel glucans from Morchella importuna fruiting bodies. Int. J. Biol. Macromol. 183, 145–157 (2021).

    Google Scholar 

  11. Xu, Y. et al. Morchella importuna flavones improve intestinal integrity in dextran sulfate sodium-challenged mice. Front. Microbiol. 12, 742033 (2021).

    Google Scholar 

  12. Pan, X. et al. In-depth investigation of the hypoglycemic mechanism of Morchella importuna polysaccharide via metabonomics combined with 16S rRNA sequencing. Int. J. Biol. Macromol. 220, 659–670 (2022).

    Google Scholar 

  13. Amangeldinova, M. et al. Simultaneous quantitative screening of 53 phytochemicals from rheum Tataricum L. roots: a comparative study of supercritical CO2, subcritical ethanol, and ultrasound-assisted extraction for enhanced antioxidant, antibacterial activities, and molecular Docking study. Front. Plant Sci. 15, 1513875 (2024).

    Google Scholar 

  14. Necip, A. & Durgun, M. Antioxidant properties, total phenolic content and LC-MS/MS analysis of mentha pulegium, lepidium Draba and Centaurea solstitialis. J. Inst. Sci. Technol. 12, 2375–2385 (2022).

    Google Scholar 

  15. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37, 277–285 (2004).

    Google Scholar 

  16. Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 38, 1103–1111 (2005).

    Google Scholar 

  17. Sevindik, M. Anticancer, antimicrobial, antioxidant and DNA protective potential of mushroom leucopaxillus Gentianeus (Quél.) Kotl. Indian J. Experimental Biology (IJEB). 59, 310–315 (2021).

    Google Scholar 

  18. Ünal, O. et al. Optimization of Phellinus hartigii extracts: biological activities, and phenolic content analysis. BMC Complement. Med. Ther. 25, 113 (2025).

    Google Scholar 

  19. Ellman, G. L., Courtney, K. D., Andres Jr, V. & Featherstone, R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95 (1961).

    Google Scholar 

  20. Al Qutaibi, M. & Kagne, S. R. Exploring the phytochemical compositions, antioxidant activity, and nutritional potentials of edible and medicinal mushrooms. Int. J. Microbiol., 2024, 6660423. (2024).

  21. Semaming, Y., Pannengpetch, P., Chattipakorn, S. C. & Chattipakorn, N. Pharmacological properties of protocatechuic acid and its potential roles as complementary medicine. Evidence-Based Complementary and Alternative Medicine 2015, 593902. (2015).

  22. Mohammed, F. S., Sevindik, M., Uysal, İ. & Sabik, A. E. Quercetin: derivatives, biosynthesis, biological activity, Pharmacological and therapeutic effects. Prospects Pharm. Sci. 21, 49–56 (2023).

    Google Scholar 

  23. Yazar, M., Sevindik, M., Uysal, I. & Polat, A. O. Effects of caffeic acid on human health: Pharmacological and therapeutic Effects, biological activity and toxicity. Pharm. Chem. J. 59, 44–55 (2025).

    Google Scholar 

  24. Liao, X. et al. Cellular antioxidant and antiproliferative activities of Morchella conica Pers. Polyphenols in vitro. J. Food Nutr. Res. 5, 742–749 (2017).

    Google Scholar 

  25. Wagay, J. A. et al. Phenolic profiling and antioxidant capacity of Morchella esculenta L. by chemical and electrochemical methods at multiwall carbon nanotube paste electrode. J. Food Meas. Charact. 13, 1805–1819 (2019).

    Google Scholar 

  26. Acar, İ. et al. The phenolic profile and biological activities of the wild-edible mushrooms Helvella Leucopus and Morchella pulchella. J. Food Meas. Charact. 15, 555–566 (2021).

    Google Scholar 

  27. Zhai, F. H., Yan, M. Q. & Wang, Y. Extraction optimization, identification using UPLC–tandem mass spectrometry, and antioxidant properties of polyphenols from the fruit body of Morchella sextelata. J. Food Sci. 89, 9214–9229 (2024).

    Google Scholar 

  28. Tian, Y. et al. Extraction and optimization of polyphenols from Morchella spp. Using ultrasound-assisted deep eutectic solvents: potential intervention for type 2 diabetes mellitus. J. Food Sci. 90, e70145 (2025).

    Google Scholar 

  29. Joshi, T., Deepa, P. & Sharma, P. K. Effect of different proportions of phenolics on antioxidant potential: Pointers for bioactive Synergy/Antagonism in foods and nutraceuticals. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 92, 939–946 (2022).

  30. Skroza, D. et al. Investigation of antioxidant synergisms and antagonisms among phenolic acids in the model matrices using FRAP and ORAC methods. Antioxidants 11, 1784 (2022).

    Google Scholar 

  31. Abdelkader, M. A. E., Mediatrice, H., Lin, D., Lin, Z. & Aggag, S. A. Mitigating oxidative stress and promoting cellular longevity with mushroom extracts. Foods 13, 4028 (2024).

    Google Scholar 

  32. Liu, Q., Zhao, Z., Dong, H. & Dong, C. Reactive oxygen species induce sclerotial formation in Morchella importuna. Appl. Microbiol. Biotechnol. 102, 7997–8009 (2018).

    Google Scholar 

  33. Wang, F. et al. DPPH radical scavenging activity of new phenolics from the fermentation broth of mushroom Morehella importuna. Molecules 28, 4760 (2023).

    Google Scholar 

  34. Necip, A. et al. Isolation of phenolic compounds from eco-friendly white bee propolis: Antioxidant, wound‐healing, and anti‐Alzheimer effects. Food Sci. Nutr. 12, 1928–1939 (2024).

    Google Scholar 

  35. Eraslan, E. C. et al. Some biological activities and element contents of ethanol extract of wild edible mushroom Morchella esculenta. Sigma J. Eng. Nat. Sci. 39, 24–28 (2021).

    Google Scholar 

  36. Sevindik, M., Gürgen, A., Korkmaz, A. F. & Akata, I. Optimizing Ultrasonic-Assisted extraction process of paralepista flaccida: A comparative study of Antioxidant, Anticholinesterase, and antiproliferative activities via response surface methodology and artificial neural network modeling. Molecules 30, 3317 (2025).

    Google Scholar 

  37. Gürgen, A. & Sevindik, M. Single and Multi-Objective optimization of the red pine mushroom lactarius deliciosus (Agaricomycetes) extraction conditions using artificial intelligence methods and biological activities of optimized extracts. Int. J. Med. Mushrooms. 27, 59–73 (2025).

    Google Scholar 

  38. Sevindik, M., Gürgen, A., Khassanov, V. T. & Bal, C. Biological activities of ethanol extracts of hericium Erinaceus obtained as a result of optimization analysis. Foods 13, 1560 (2024).

    Google Scholar 

  39. Gürgen, A., Unal, O. & Sevindik, M. Biological activities of the golden Chantarelle mushroom Cantharellus cibarius (Agaricomycetes) extracts obtained as a result of single and multi-objective optimization studies. Int. J. Med. Mushrooms. 26, 63–74 (2024).

    Google Scholar 

  40. Wei, Y. M. et al. Triterpenoids as bivalent and dual inhibitors of acetylcholinesterase/butyrylcholinesterase from the fruiting bodies of inonotus obliquus. Phytochemistry 200, 113182 (2022).

    Google Scholar 

  41. Švestková, P., Balík, J. & Soural, I. Synergistic effect of selected carboxylic acids and phenolic compounds detected by the FRAP method. Food Chemistry: X. 23, 101573 (2024).

    Google Scholar 

  42. Badshah, S. L. et al. Isolation, characterization, and medicinal potential of polysaccharides of Morchella esculenta. Molecules 26, 1459 (2021).

    Google Scholar 

  43. İzol, E. et al. Research of peganum harmala: phytochemical Content, mineral Profile, Antioxidant, Antidiabetic, anticholinergic Properties, and molecular Docking. Chemistry & Biodiversity, 22, e202403178 (2025).

  44. Necip, A. Cornus Mas L. Seeds as a potent natural antioxidant source: insights from phenolic profiling and antioxidant capacity assays. J. Oleo Sci. 74, 897–908 (2025).

    Google Scholar 

  45. Çirak, O., Necip, A., Isık, M. & Beydemir, Ş. Antioxidant, anti-acetylcholinesterase potentials, ADME estimations and molecular Docking studies of green algae extracts. Eur. J. Life Sci. 4, 24–35 (2025).

    Google Scholar 

  46. Yildirim, M., Necip, A., Cimentepe, M., Emire, Z. & Yabalak, E. Bioactive potential of Origanum munzurense kit Tan & Sorger extracts: Antioxidant, anticancer, and antibacterial activities explored through Sc-CO2 extraction and molecular Docking analysis. Journal Chromatogr. B, 1259, 124614 (2025).

  47. Wasser, S. P. Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and Immunomodulatory activities: a review. International J. Med. Mushrooms 19, 279–317 (2017).

  48. Haq, F. U., Imran, M., Saleem, S., Rafi, A. & Jamal, M. Investigation of three Morchella species for anticancer activity against colon cancer cell lines by UPLC-MS-based chemical analysis. Appl. Biochem. Biotechnol. 195, 486–504 (2023).

    Google Scholar 

  49. Xiong, C. et al. Induction of apoptosis in HeLa cells by a novel peptide from fruiting bodies of Morchella importuna via the mitochondrial apoptotic pathway. Evidence-Based Complementary and Alternative Medicine 2021, 5563367. (2021).

  50. Haq, F. U. et al. Chemical characterization and cytotoxic effect of three edible fungi (Morchella) against breast cancer cells: A therapeutic approach. Kuwait J. Sci. 52, 100285 (2025).

    Google Scholar 

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