Bacterial nanocellulose films functionalized with Janus nanoparticles: Preparation and application in chicken meat preservation and safety

1. Javan, A. J. et al. Effect of vicia villosa protein isolate-based edible coating incorporated with ZnO nanoparticles on the shelf-life of chicken breast meat during cold storage. Food Hydrocoll. Heal. 5, 100176 (2024).

   Google Scholar 

2. Yaghoubi, M. et al. Effect of Chitosan coating incorporated with Artemisia Fragrans essential oil on fresh chicken meat during refrigerated storage. Polym. (Basel). 13, 716 (2021).

   Google Scholar 

3. Thung, T. Y. et al. Prevalence and antibiotic resistance of Salmonella enteritidis and Salmonella typhimurium in Raw chicken meat at retail markets in Malaysia. Poult. Sci. 95, 1888–1893 (2016).

   Google Scholar 

4. Liu, Z. et al. Janus particles: A review of their applications in food and medicine. Crit. Rev. Food Sci. Nutr. 63, 10093–10104 (2023).

   Google Scholar 

5. Lotfi Javid, A., Almasi, H., Molaei, R. & Moradi, M. Basil essential oil-incorporated bacterial nanocellulose foam: Fabrication, characterization, and application in extending ground beef shelf life. J Agric. Food Res 18, (2024).

6. Ghorbani, M., Tajik, H., Moradi, M., Molaei, R. & Alizadeh, A. One-pot microbial approach to synthesize carbon Dots from baker’s yeast-derived compounds for the Preparation of antimicrobial membrane. J. Environ. Chem. Eng. 10, 107525 (2022).

   Google Scholar 

7. Costa, L. et al. Bacterial nanocellulose as a simple and tailorable platform for controlled drug release. Int. J. Pharm. 663, 124560 (2024).

   Google Scholar 

8. Soares Silva, A. G. Performance of bacterial nanocellulose packaging film functionalised in situ with zinc oxide: migration onto chicken skin and antimicrobial activity. Food Packag Shelf Life. 39, 101140 (2023).

   Google Scholar 

9. Soares Silva, A. G. Antimicrobial activity of in-situ bacterial nanocellulose-zinc oxide composites for food packaging. Food Packag Shelf Life. 40, 101201 (2023).

   Google Scholar 

10. Riahi, Z., Khan, A., Rhim, J. W., Shin, G. H. & Kim, J. T. Sustainable packaging film based on cellulose nanofibres/pullulan impregnated with zinc-doped carbon Dots derived from avocado Peel to extend the shelf life of chicken and Tofu. Int. J. Biol. Macromol. 258, 129302 (2024).

    Google Scholar 

11. Su, H. et al. Janus particles: design, preparation, and biomedical applications. Mater. Today Bio. 4, 100033 (2019).

    Google Scholar 

12. Le, T. C., Zhai, J., Chiu, W. H. & Tran, P. A. Tran, N. Janus particles: recent advances in the biomedical applications. Int. J. Nanomed. 14, 6749–6777 (2019).

    Google Scholar 

13. Subasi, B. G., Xiao, J. & Capanogluo, E. Potential use of Janus structures in food applications. eFood 2, 279–287 (2021).

    Google Scholar 

14. Honciuc, A. Amphiphilic Janus particles at interfaces. in 95–136 (2019). https://doi.org/10.1007/978-3-030-23370-9_4

15. Safaie, N. & Ferrier, R. C. Janus nanoparticle synthesis: Overview, recent developments, and applications. J Appl. Phys 127, (2020).

16. Zhang, X., Fu, Q., Duan, H., Song, J. & Yang, H. Janus nanoparticles: from fabrication to (Bio)Applications. ACS Nano. 15, 6147–6191 (2021).

    Google Scholar 

17. Franco, M. R., da Cunha, L. R. & Bianchi, R. F. Janus principle applied to food safety: an active two-faced indicator label for tracking meat freshness. Sens. Actuators B Chem. 333, 129466 (2021).

    Google Scholar 

18. Ling, Z., Xu, Q., Li, W., Gao, J. & Xu, H. Fluorescence sensing and effective elimination of Listeria monocytogenes in food based on Janus gold carbon dots-silver nanoclusters composites. Food Biosci. 65, 106003 (2025).

    Google Scholar 

19. Du, H. et al. CO2-responsive multifunctional label based on Chitosan and hyaluronic acid for visualizing and maintaining postharvest freshness. Food Hydrocoll. 157, 110438 (2024).

    Google Scholar 

20. Jiao, X. et al. Antibacterial smart absorbent pad with Janus structure for meat preservation. Food Packag Shelf Life. 37, 101066 (2023).

    Google Scholar 

21. Fan, S. et al. Zein/chitosan Janus film incorporated with Tannic acid and cinnamon essential oil co-loaded Pickering emulsion for sustained controlled release and pork preservation. Int. J. Biol. Macromol. 286, 138429 (2025).

    Google Scholar 

22. Zhu, Z. et al. Development of chitosan/polycaprolactone-thymol Janus films with directional transport and antibacterial properties for meat preservation. Int. J. Biol. Macromol. 268, 131669 (2024).

    Google Scholar 

23. Ghorbani, M., Moradi, M., Tajik, H., Molaei, R. & Alizadeh, A. Carbon Dots embedded bacterial cellulose membrane as active packaging: Toxicity, in vitro release and application in minced beef packaging. Food Chem. 433, 137311 (2024).

    Google Scholar 

24. Razavi, R., Tajik, H., Molaei, R., McClements, D. J. & Moradi, M. Janus nanoparticles synthesized from hydrophobic carbon Dots and carboxymethyl cellulose: novel antimicrobial additives for fresh food applications. Food Biosci. 62, 105171 (2024).

    Google Scholar 

25. Varasteh, S., Braber, S., Kraneveld, A. D., Garssen, J. & Fink-Gremmels, J. l-Arginine supplementation prevents intestinal epithelial barrier breakdown under heat stress conditions by promoting nitric oxide synthesis. Nutr. Res. 57, 45–55 (2018).

    Google Scholar 

26. Shiravani, Z., Aliakbarlu, J. & Moradi, M. Application of bacterial nanocellulose film loaded with sodium nitrite, sumac, and black Carrot extracts to reduce sodium nitrite, extend shelf life, and inhibit clostridium perfringens in cooked beef Ham. Int. J. Biol. Macromol. 280, 135841 (2024).

    Google Scholar 

27. Nikfarjam, N., Razavi, R., Moradi, M. & Molaei, R. Green synthesis of carbon Dots from onion juice and Ex-Situ embedding for antimicrobial/ultraviolet protective nanocellulose films. Food Saf. Packag. 1, 24–32 (2025).

    Google Scholar 

28. Jahanbakhsh Oskouei, M., Alizadeh Khaledabad, M., Almasi, H., Hamishekar, H. & Amiri, S. Preparation and characterization of kafirin/PLA electrospun nanofibers activated by syzygium aromaticum essential oil. Polym. Bull. 81, 10061–10079 (2024).

    Google Scholar 

29. Moradi, M. et al. Trends in Food Science & Technology Review of microbiological methods for testing protein and carbohydrate-based antimicrobial food packaging. 111, 595–609 (2021).

30. Meerasri, J. et al. Synergistic effects of thyme and oregano essential oil combinations for enhanced functional properties of sericin/pectin film. Int. J. Biol. Macromol. 263, 130288 (2024).

    Google Scholar 

31. Molaei, R. et al. Hydrophobic carbon dots: an overview of the synthesis, purification, cytotoxicity, and potential applications in food safety and analytical chemistry. Qual. Assur. Saf. Crop Foods. 17, 136–164 (2025).

    Google Scholar 

32. Zhang, S. et al. Kill three birds with one stone: Mitochondria-localized tea saponin derived carbon Dots with AIE properties for stable detection of HSA and extremely acidic pH. Food Chem. 405, 134865 (2023).

    Google Scholar 

33. Razavi, R. et al. Biosynthesis of metallic nanoparticles using mulberry fruit (Morus Alba L.) extract for the Preparation of antimicrobial nanocellulose film. Appl. Nanosci. 10, 465–476 (2020).

    Google Scholar 

34. Salimi, F., Moradi, M., Tajik, H. & Molaei, R. Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon Dots of white mulberry (< scp> Morus Alba  L). J. Sci. Food Agric. 101, 3439–3447 (2021).

    Google Scholar 

35. Khan, H. et al. Biotransformation of banana Peel waste into bacterial nanocellulose and its modification for active antimicrobial packaging using Polyvinyl alcohol with in-situ generated silver nanoparticles. Food Packag Shelf Life. 38, 101115 (2023).

    Google Scholar 

36. Mirtalebi, S. S., Almasi, H. & Alizadeh Khaledabad, M. Physical, morphological, antimicrobial and release properties of novel MgO-bacterial cellulose nanohybrids prepared by in-situ and ex-situ methods. Int. J. Biol. Macromol. 128, 848–857 (2019).

    Google Scholar 

37. Hosseiniyeh, N., Mohtarami, F., Almasi, H. & Azizi, S. Soy protein isolate film activated by black seed oil nanoemulsion as a novel packaging for shelf-life extension of bulk bread. Food Sci. Nutr. 12, 1706–1723 (2024).

    Google Scholar 

38. De Cicco, D., Asaee, Z. & Taheri, F. Use of nanoparticles for enhancing the interlaminar properties of Fiber-Reinforced composites and adhesively bonded Joints—A review. Nanomaterials 7, 360 (2017).

    Google Scholar 

39. Pyromali, C. et al. Nonmonotonic composition dependence of viscosity upon adding Single-Chain nanoparticles to entangled polymers. Macromolecules 57, 4826–4832 (2024).

    Google Scholar 

40. Ezati, P. et al. Preparation and characterization of B, S, and N-doped glucose carbon dots: Antibacterial, antifungal, and antioxidant activity. Sustain. Mater. Technol. 32, e00397 (2022).

    Google Scholar 

41. Mousavi Khaneghah, A., Hashemi, S. M. B. & Limbo, S. Antimicrobial agents and packaging systems in antimicrobial active food packaging: an overview of approaches and interactions. Food Bioprod. Process. 111, 1–19 (2018).

    Google Scholar 

42. Ghosal, K. et al. Antibacterial photodynamic activity of hydrophobic carbon quantum Dots and Polycaprolactone based nanocomposite processed via both electrospinning and solvent casting method. Photodiagnosis Photodyn Ther. 35, 102455 (2021).

    Google Scholar 

43. Yuan, L. et al. Janus biopolymer nanocomposite coating with excellent antibacterial and water/oxygen barrier performance for fruit preservation. Food Hydrocoll. 149, 109528 (2024).

    Google Scholar 

44. Ma, Z. et al. Lotus leaf inspired sustainable and multifunctional Janus film for food packaging. Chem. Eng. J. 457, 141279 (2023).

    Google Scholar 

45. Rahman, M. S. et al. Recent developments of carboxymethyl cellulose. Polym. (Basel). 13, 1345 (2021).

    Google Scholar 

46. Martinello, M. & Mutinelli, F. Antioxidant activity in bee products: A review. Antioxidants 10, 71 (2021).

    Google Scholar 

47. Murueva, A. V. et al. Biodegradable polymer casting films for drug delivery and cell culture. Giant 19, 100314 (2024).

    Google Scholar 

48. Dang, R., Xu, J., Zhang, B., Zhao, S. & Dang, Y. Preparation of bacterial cellulose-based antimicrobial materials and their applications in wound dressing: A review. Mater. Des. 253, 113820 (2025).

    Google Scholar 

49. Khorshidi, S., Mehdizadeh, T., Tajik, H., Hamishekar, H. & Reale, A. Characterization of Lactiplantibacillus Plantarum subsp. Plantarum and bifidobacterium animalis spp. Lactis BB-12 postbiotics: in vivo and in vitro experiments against foodborne pathogens. LWT 224, 117823 (2025).

    Google Scholar 

50. Lin, L. et al. Preparation and characterization of gelatin active packaging film loaded with Eugenol nanoparticles and its application in chicken preservation. Food Biosci. 53, 102778 (2023).

    Google Scholar 

51. Sasikumar, T. et al. Functional composite films incorporating triphala-derived carbon Dots for extending chicken preservation. Int. J. Biol. Macromol. 280, 135856 (2024).

    Google Scholar 

52. Yousefi, M., Farshidi, M. & Ehsani, A. Effects of lactoperoxidase system-alginate coating on chemical, microbial, and sensory properties of chicken breast fillets during cold storage. J Food Saf 38, (2018).

53. Nouri Ala, M. A. & Shahbazi, Y. The effects of novel bioactive carboxymethyl cellulose coatings on food-borne pathogenic bacteria and shelf life extension of fresh and sauced chicken breast fillets. LWT 111, 602–611 (2019).

    Google Scholar 

54. Mehdizadeh, T. & Mojaddar Langroodi, A. Chitosan coatings incorporated with propolis extract and Zataria multiflora Boiss oil for active packaging of chicken breast meat. Int. J. Biol. Macromol. 141, 401–409 (2019).

    Google Scholar 

55. Wang, W. et al. Biodegradable cellulose/curcumin films with Janus structure for food packaging and freshness monitoring. Carbohydr. Polym. 324, 121516 (2024).

    Google Scholar 

56. Moura-Alves, M., Esteves, A., Ciríaco, M., Silva, J. A. & Saraiva, C. Antimicrobial and antioxidant edible films and coatings in the Shelf-Life improvement of chicken meat. Foods 12, 2308 (2023).

    Google Scholar 

57. Zhou, X. et al. Effect of Konjac glucomannan/carrageenan-based edible emulsion coatings with camellia oil on quality and shelf-life of chicken meat. Int. J. Biol. Macromol. 183, 331–339 (2021).

    Google Scholar 

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