Biopolymer-based multilayer capsules for protection and controlled release of Pseudomonas fluorescens T17-4 and Bacillus velezensis VRU1

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biopolymer-based-multilayer-capsules-for-protection-and-controlled-release-of-pseudomonas-fluorescens-t17-4-and-bacillus-velezensis-vru1
Biopolymer-based multilayer capsules for protection and controlled release of Pseudomonas fluorescens T17-4 and Bacillus velezensis VRU1

References

  1. Glick, B. R. Plant growth-promoting bacteria: mechanisms and applications. Scientifica 2012, 963401 (2012).

  2. Ali, S. S. et al. Advances in microorganisms-based biofertilizers: major mechanisms and applications. In Biofertilizers: Volume 1 – Advances in Bio-Inoculants (eds Rakshit, A. et al) 371–385 (Woodhead Publishing, 2021).

  3. Orozco-Mosqueda, M. C. et al. Plant growth-promoting bacteria as bioinoculants: attributes and challenges for sustainable crop improvement. Agronomy 11, 1167 (2021).

    Google Scholar 

  4. Reque, P. M. & Brandelli, A. Encapsulation of probiotics and nutraceuticals: applications in functional food industry. Trends Food Sci. Technol. 114, 1–10 (2021).

    Google Scholar 

  5. Oliveira, M. B., Hatami, J. & Mano, J. F. Coating strategies using layer-by‐layer deposition for cell encapsulation. Chem. Asian J. 11, 1753–1764 (2016).

    Google Scholar 

  6. Cai, M., Yang, J., Lu, X. & Lu, X. Layer-by-layer self-assembly strategies of atomically thin two-dimensional nanomaterials: principles, methods, and functional applications. ACS Appl. Nano Mater. 7, 27940–27959 (2024).

    Google Scholar 

  7. Liu, X. et al. Recent advances in layer-by-layer assembly scaffolds for co-delivery of bioactive molecules for bone regeneration: an updated review. J. Transl Med. 22, 1001 (2024).

    Google Scholar 

  8. Talebian, S. et al. Biopolymer-based multilayer microparticles for probiotic delivery to colon. Adv. Healthc. Mater. 11, 2102487 (2022).

    Google Scholar 

  9. Zhang, F. et al. Enhanced viability of probiotics in composite hydrogel beads. J. Food Eng. 357, 111621 (2023).

    Google Scholar 

  10. Fathi, F., Saberi-Riseh, R. & Khodaygan, P. Survivability and controlled release of alginate-microencapsulated Pseudomonas fluorescens VUPF506 and their effects on biocontrol of Rhizoctonia Solani on potato. Int. J. Biol. Macromol. 183, 627–634 (2021).

    Google Scholar 

  11. Pour, M. M., Hassanisaadi, M., Kennedy, J. F. & Riseh, R. S. A novel biopolymer technique for encapsulation of Bacillus velezensis BV9 into double coating biopolymer made by in alginate and natural gums to biocontrol of wheat take-all disease. Int. J. Biol. Macromol. 257, 128526 (2024).

    Google Scholar 

  12. Liu, B., Hu, J., Yao, H., Zhang, L. & Liu, H. Improved viability of probiotics encapsulated by layer-by-layer assembly using zein nanoparticles and pectin. Food Hydrocoll. 143, 108899 (2023).

    Google Scholar 

  13. Hernández-Gallegos, M. A. et al. Protective effect of alginate microcapsules with different rheological behavior on Lactiplantibacillus plantarum 299v. Gels 9, 682 (2023).

    Google Scholar 

  14. Sun, R. et al. Preparation and characterization of pectin-alginate-based microbeads reinforced by nano montmorillonite filler for probiotics encapsulation: improving viability and colonic colonization. Int. J. Biol. Macromol. 264, 130543 (2024).

    Google Scholar 

  15. Riaz, T. et al. In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads. J. microencapsul. 36, 192–203 (2019).

    Google Scholar 

  16. Sun, X. et al. Maillard-type protein–polysaccharide conjugates and electrostatic protein–polysaccharide complexes as delivery vehicles for food bioactive ingredients: formation, types, and applications. Gels 8, 135 (2022).

    Google Scholar 

  17. Hugues-Ayala, A. M., Sarabia-Sainz, J. A., González-Rios, H., Vázquez-Moreno, L. & Montfort, G. R. C. Airbrush encapsulation of Lactobacillus rhamnosus GG in dry microbeads of alginate coated with regular buttermilk proteins. LWT 117, 108639 (2020).

    Google Scholar 

  18. Tanwar, M., Gupta, R. K. & Rani, A. Natural gums and their derivatives based hydrogels: in biomedical, environment, agriculture, and food industry. Crit. Rev. Biotechnol. 44, 275–301 (2024).

    Google Scholar 

  19. Moradi Pour, M. et al. Microencapsulation of Bacillus velezensis using alginate-gum polymers enriched with TiO2 and SiO2 nanoparticles. Micromachines 13, 1423 (2022).

    Google Scholar 

  20. Panyachanakul, T. et al. New insight into thermo-solvent tolerant lipase produced by Streptomyces sp. A3301 for re-polymerization of Poly (DL-lactic acid). Polymer 204, 122812 (2020).

    Google Scholar 

  21. Kasana, R. C., Salwan, R., Dhar, H., Dutt, S. & Gulati, A. A rapid and easy method for the detection of microbial cellulases on agar plates using gram’s iodine. Curr. microbiol. 57, 503–507 (2008).

    Google Scholar 

  22. Maurhofer, M., Keel, C., Haas, D. & Défago, G. Influence of plant species on disease suppression by Pseudomonas fluorescens strain CHAO with enhanced antibiotic production. Plant. Pathol. 44, 40–50 (1995).

    Google Scholar 

  23. Alström, S. Factors associated with detrimental effects of rhizobacteria on plant growth. Plant. Soil. 102, 3–9 (1987).

    Google Scholar 

  24. Son, H. J., Park, G. T., Cha, M. S. & Heo, M. S. Solubilization of insoluble inorganic phosphates by a novel salt-and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresour Technol. 97, 204–210 (2006).

    Google Scholar 

  25. Patten, C. L. & Glick, B. R. Bacterial biosynthesis of indole-3-acetic acid. Can. J. Microbiol. 42, 207–220 (1996).

    Google Scholar 

  26. Teng, Z., Han, Y., Li, J., Yan, F. & Yang, W. Preparation of Hollow mesoporous silica spheres by a sol–gel/emulsion approach. Microporous Mesoporous Mater. 127, 67–72 (2010).

    Google Scholar 

  27. Chávarri, M. et al. Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int. J. Food Microbiol. 142, 185–189 (2010).

    Google Scholar 

  28. Tu, L., He, Y., Yang, H., Wu, Z. & Yi, L. Preparation and characterization of alginate–gelatin microencapsulated Bacillus subtilis SL-13 by emulsification/internal gelation. J. Biomater. Sci. Polym. Ed. 26, 735–749 (2015).

    Google Scholar 

  29. Wu, Z., Guo, L., Qin, S. & Li, C. Encapsulation of R. planticola Rs-2 from alginate-starch-bentonite and its controlled release and swelling behavior under simulated soil conditions. J. Ind. Microbiol. Biotechnol. 39, 317–327 (2012).

    Google Scholar 

  30. Alfawaz, A., Alsalme, A., Alkathiri, A. & Alswieleh, A. Surface functionalization of mesoporous silica nanoparticles with brønsted acids as a catalyst for esterificatsion reaction. J. King Saud Univ. Sci. 34, 102106 (2022).

    Google Scholar 

  31. Yi, H., Yang, Y., Gu, X., Huang, J. & Wang, C. Multilayer composite microcapsules synthesized by Pickering emulsion templates and their application in self-healing coating. J. Mater. Chem. 3, 13749–13757 (2015).

    Google Scholar 

  32. McClements, D. J. Advances in fabrication of emulsions with enhanced functionality using structural design principles. Curr. Opin. Colloid Interface Sci. 17, 235–245 (2012).

    Google Scholar 

  33. Noureen, S. et al. Prunus armeniaca gum-alginate polymeric microspheres to enhance the bioavailability of tramadol hydrochloride: formulation and evaluation. Pharmaceutics 14, 916 (2022).

    Google Scholar 

  34. Samajdar, S. & Kumar, K. J. Impact on stabilization of ionic gum emulsions by natural and thermally modified chironji gum. Int. J. Biol. Macromol. 156, 233–238 (2020).

    Google Scholar 

  35. de Souza, W. F. C. et al. Incorporation and influence of natural gums in an alginate matrix for Serratia plymuthica immobilization and isomaltulose production. Food Res. Int. 162, 112050 (2022).

    Google Scholar 

  36. Muley, A. B., Pandit, A. B., Singhal, R. S. & Dalvi, S. G. Production of biologically active peptides by hydrolysis of whey protein isolates using hydrodynamic cavitation. Ultrason. Sonochem. 71, 105385 (2021).

    Google Scholar 

  37. Chen, J. et al. Characterization of whey protein Isolate–Soymilk complexes modified by transglutaminase and their application InYuba film. Foods 14, 2916 (2025).

    Google Scholar 

  38. Mishra, R., Majeed, A. & Banthia, A. Development and characterization of pectin/gelatin hydrogel membranes for wound dressing. Int. J. Plast. Technol. 15, 82–95 (2011).

    Google Scholar 

  39. Hu, W., Chen, S., Wu, D., Zhu, K. & Ye, X. Physicochemical and macromolecule properties of RG-I enriched pectin from citrus wastes by manosonication extraction. Int. J. Biol. Macromol. 176, 332–341 (2021).

    Google Scholar 

  40. Dehkordi, S. S., Alemzadeh, I., Vaziri, A. S. & Vossoughi, A. Optimization of alginate-whey protein isolate microcapsules for survivability and release behavior of probiotic bacteria. Appl. Biochem. Biotechnol. 190, 182–196 (2020).

    Google Scholar 

  41. Hamid, S. et al. Biopolymer-based microencapsulation of bioactive compounds: evaluation of the impact of encapsulated compound characteristics on process efficiency. Surfaces 8, 15 (2025).

    Google Scholar 

  42. Du, Q., Zhou, L., Lyu, F., Liu, J. & Ding, Y. The complex of whey protein and pectin: interactions, functional properties and applications in food colloidal systems—a review. Colloids Surf. B Biointerfaces. 210, 112253 (2022).

    Google Scholar 

  43. Venkatesan, R., Sekar, S., Raorane, C. J., Raj, V. & Kim, S. C. Hydrophilic composites of chitosan with almond gum: characterization and mechanical, and antimicrobial activity for compostable food packaging. Antibiotics 11, 1502 (2022).

    Google Scholar 

  44. Çoklar, H. & Akbulut, M. Physicochemical, phytochemical, structural, and rheological characterisation and chemometric classification of exudate gums of five Prunus armeniaca varieties. Int. J. Biol. Macromol. 321, 146392 (2025).

    Google Scholar 

  45. Koşar, M., Uluata, S., Durmaz, G. & Kadkhodaee, R. Malatya apricot gum: a source of natural gum and its physicochemical, functional and antioxidant properties. Int. J. Biol. Macromol. 301, 140447 (2025).

    Google Scholar 

  46. Gbassi, G. et al. Whey proteins analysis in aqueous medium and in artificial gastric and intestinal fluids. Int. J. Biol. Chem. Sci. 6, 1828–1837 (2012).

    Google Scholar 

  47. Pereira, R., Tojeira, A., Vaz, D. C., Mendes, A. & Bártolo, P. Preparation and characterization of films based on alginate and aloe vera. Int. J. Polym. Anal. Charact. 16, 449–464 (2011).

    Google Scholar 

  48. Yao, C. et al. Flotation separation of cassiterite from calcite using low-molecular-weight citrus pectin as depressant. Separations 11, 95 (2024).

    Google Scholar 

  49. Guerrero, P., Kerry, J. P. & de la Caba, K. FTIR characterization of protein–polysaccharide interactions in extruded blends. Carbohydr. Polym. 111, 598–605 (2014).

    Google Scholar 

  50. Ozdemir, N. et al. Microencapsulation of basil essential oil: utilization of gum arabic/whey protein isolate/maltodextrin combinations for encapsulation efficiency and in vitro release. J. Food Meas. Charact. 15, 1865–1876 (2021).

    Google Scholar 

  51. Sun, Y., Zhang, M., Bhandari, B. & Bai, B. Fennel essential oil loaded porous starch-based microencapsulation as an efficient delivery system for the quality improvement of ground pork. Int. J. Biol. Macromol. 172, 464–474 (2021).

    Google Scholar 

  52. Saberi Riseh, R., Fathi, F. & Moradi, M. The effects of biocontrol Bacillus and Pseudomonas strains on plant growth and biochemical defense echanisms in pistachio seedlings inoculated with Phytophthora drechsleri. Pistachio Health J. 1, 15–26 (2019).

    Google Scholar 

  53. Saberi-Rise, R. & Moradi-Pour, M. The effect of Bacillus subtilis Vru1 encapsulated in alginate–bentonite coating enriched with titanium nanoparticles against Rhizoctonia solani on bean. Int. J. Biol. Macromol. 152, 1089–1097 (2020).

    Google Scholar 

  54. Saberi, R. R., Fathi, F. & Moradzadeh, E. M. The effect of some probiotic bacteria in induction of drought tolerance in cucumber plants. Microbiol. Metab. Biotechnol. 2, 49–63 (2019).

    Google Scholar 

  55. Ajinde, A. O., Dayo-Olagbende, O. G. & Akpor, O. B. in 2024 International conference on science, engineering and business for driving sustainable development goals (SEB4SDG). 1–16IEEE, (2024).

  56. Khalil, M. S. M., Hassan, M. H. A. R., Mahmoud, A. F. & Morsy, K. M. M. Involvement of secondary metabolites and extracellular lytic enzymes produced by plant growth promoting rhizobacteria in inhibiting the soilborne pathogens in Faba bean plants. Jurnal J. Trop. Plant. Pests Dis. 22, 100–108 (2022).

    Google Scholar 

  57. Yasmin, I., Saeed, M., Pasha, I. & Zia, M. A. Development of Whey protein concentrate-pectin-alginate based delivery system to improve survival of B. longum BL-05 in simulated Gastrointestinal conditions. Probiotics Antimicrob. Proteins. 11, 413–426 (2019).

    Google Scholar 

  58. Karimi, M., Sekhavatizadeh, S. S. & Hosseinzadeh, S. Milk dessert containing Lactobacillus reuteri (ATCC 23272) encapsulated with sodium alginate, ferula assa-foetida and Zedo (Amygdalus scoparia) gum as three layers of wall materials. Food Bioprod. Process. 127, 244–254 (2021).

    Google Scholar 

  59. Xie, A. et al. Polysaccharides, proteins, and their complex as microencapsulation carriers for delivery of probiotics: a review on carrier types and encapsulation techniques. Int. J. Biol. Macromol. 242, 124784 (2023).

    Google Scholar 

  60. Wei, H. et al. Activation of a passive, mesoporous silica nanoparticle layer through attachment of bacterially-derived carbon-quantum-dots for protection and functional enhancement of probiotics. Mater. Today Bio. 15, 100293 (2022).

    Google Scholar 

  61. Anal, A. K. & Singh, H. Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends Food Sci. Technol. 18, 240–251 (2007).

    Google Scholar 

  62. Jeong, Y. & Irudayaraj, J. Multi-layered alginate hydrogel structures and bacteria encapsulation. Chem. Commun. 58, 8584–8587 (2022).

    Google Scholar 

  63. Luo, X. et al. Encapsulation of Escherichia coli strain Nissle 1917 in a chitosan―alginate matrix by combining layer-by-layer assembly with CaCl2 cross-linking for an effective treatment of inflammatory bowel diseases. Colloids Surf. B Biointerfaces. 189, 110818 (2020).

    Google Scholar 

  64. Saberi Riseh, R., Moradi Pour, M. & Ait Barka, E. A novel route for double-layered encapsulation of Streptomyces fulvissimus Uts22 by alginate–Arabic gum for controlling of Pythium aphanidermatum in cucumber. Agronomy 12, 655 (2022).

    Google Scholar 

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