Three-dimensional reconstruction of a biliary system in a bioengineered liver using decellularized scaffold

3D:

Three-dimensional

BD:

Bile duct

ELISA:

Enzyme-linked immunosorbent assay

EM:

Expansion medium

ESLD:

End-stage liver disease

GFP:

Green fluorescent protein

ICO:

Intrahepatic cholangiocyte organoids

PH:

Primary hepatocytes

PV:

Portal vein

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Authors and Affiliations

1. Division of Hepato-Biliary-Pancreatic Surgery and Transplantation, Department of Surgery, Graduate School of Medicine, Kyoto University, 54 Shogo-in Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan

   Hiroshi Horie, Ken Fukumitsu, Yusuke Hanabata, Takuma Karasuyama, Kentaro Iwaki, Fumiaki Munekage, Kenta Makino, Takashi Ito, Katsuhiro Tomofuji, Hidenobu Kojima, Satoshi Ogiso, Elena Yukie Uebayashi, Takamichi Ishii & Etsuro Hatano

2. Department of Surgery, Kyoto Katsura Hospital, 17 Yamadahirao-cho, Nisigyo-ku, Kyoto, 615-8256, Japan

   Ken Fukumitsu

3. Department of Clinical Bio-Resource Research and Development, Graduate School of Medicine, Kyoto University, 46-29 Yosida-shimo-adachi-cho, Sakyo-ku, Kyoto, 606- 8501, Japan

   Hiroyuki Uematsu, Roberto Coppo, Kunishige Onuma & Masahiro Inoue

Contributions

Conceptualization: Ken Fukumitsu. Data curation: Hiroshi Horie, Ken Fukumitsu, Yusuke Hanabata, Takuma Karasuyama, Kentaro Iwaki, Fumiaki Munekage, Kenta Makino, Takashi Ito, Katsuhiro Tomofuji, Hiroyuki Uematsu, Robert Coppo, Kunishige Onuma, Masahiro Inoue. Funding acquisition : Hiroshi Horie, Ken Fukumitsu. Investigation : Hiroshi Horie, Ken Fukumitsu, Yusuke Hanabata, Takuma Karasuyama, Kentaro Iwaki, Fumiaki Munekage, Kenta Makino, Takashi Ito, Katsuhiro Tomofuji, Hidenobu Kojima, Satoshi Ogiso, Elena Yukie Uebayashi, Hiroyuki Uematsu, Robert Coppo, Kunishige Onuma, Masahiro Inoue, Takamichi Ishii, Etsuro Hatano. Project administration : Ken Fukumitsu. Resources: Hiroshi Horie, Ken Fukumitsu, Masahiro Inoue. Supervision : Masahiro Inoue, Takamichi Ishii, Etsuro Hatano. Writing—original graft: Hiroshi Horie, Ken Fukumitsu. Writing—review and editing: Satoshi Ogiso, Masahiro Inoue, Takamichi Ishii, Etsuro Hatano.

Corresponding author

Correspondence to Ken Fukumitsu.

Competing interests

The authors declare no competing interests.

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