Biodegradable lean Mg-Dy alloy enhances bone repair in senile osteoporotic fractures by modulating macrophage mediated immune microenvironment

References

Song, Q. et al. Risk factors for osteoporosis in the elderly and predictive value of age, body mass index, and visceral fat area. Nutr. Res Pract. 19, 375–385 (2025).

Google Scholar
Compston, J. E., McClung, M. R. & Leslie, W. D. Osteoporosis. Lancet 393, 364–376 (2019).

Google Scholar
Rachner, T. D., Khosla, S. & Hofbauer, L. C. Osteoporosis: now and the future. Lancet 377, 1276–1287 (2011).

Google Scholar
Zheng, L. et al. Bone targeting antioxidative nano-iron oxide for treating postmenopausal osteoporosis. Bioact. Mater. 14, 250–261 (2022).

Google Scholar
Tian, R.-C., Zhang, R.-Y. & Ma, C.-F. Rejuvenation of bone marrow mesenchymal stem cells: mechanisms and their application in senile osteoporosis treatment. Biomolecules 15, 276 (2025).

Google Scholar
Pignolo, R. J., Law, S. F. & Chandra, A. Bone aging, cellular senescence, and osteoporosis. JBMR Plus 5, 10488 (2021).

Google Scholar
Li, J. et al. Hydrogel delivery of DNase I and liposomal vancomycin to eradicate fracture-related methicillin-resistant staphylococcus aureus infection and support osteoporotic fracture healing. Acta Biomater. 164, 223–239 (2023).

Google Scholar
Li, J. et al. Fracture-related infection in osteoporotic bone causes more severe infection and further delays healing. Bone Jt. Res. 11, 49–60 (2022).

Google Scholar
Ren, L., Xu, X., Liu, H., Yang, K. & Qi, X. Biocompatibility and Cu ions release kinetics of copper-bearing titanium alloys. J. Mater. Sci. Technol. 95, 237–248 (2021).

Google Scholar
Campbell, P. G. et al. PEEK versus titanium cages in lateral lumbar interbody fusion: a comparative analysis of subsidence. Neurosurg. Focus FOC 49, E10 (2020).

Google Scholar
Zhang, Y., Fu, S., Yang, L., Qin, G. & Zhang, E. A nano-structured TiO2/CuO/Cu2O coating on Ti-Cu alloy with dual function of antibacterial ability and osteogenic activity. J. Mater. Sci. Technol. 97, 201–212 (2022).

Google Scholar
Lee, H. et al. 3D-printed tissue-specific nanospike-based adhesive materials for time-regulated synergistic tumor therapy and tissue regeneration in vivo. Adv. Funct. Mater. 34, 2406237 (2024).

Google Scholar
Al-Tamimi, A. A., Quental, C., Folgado, J., Peach, C. & Bartolo, P. Stress analysis in a bone fracture fixed with topology-optimised plates. Biomech. Model. Mechanobiol. 19, 693–699 (2020).

Google Scholar
Wu, Y. et al. Electrophoretic deposition of MXene coating on Mg alloy 2,5 PDCA-LDH film for enhanced anticorrosion/wear. Colloids Surf. A Physicochem. Eng. Asp. 702, 135043 (2024).

Google Scholar
Ji, H. et al. Biodegradable Zn-2Cu-0.5Zr alloy promotes the bone repair of senile osteoporotic fractures via the immune-modulation of macrophages. Bioact. Mater. 38, 422–437 (2024).

Google Scholar
Zheng, Y., Ruan, Z., Liu, S., Yang, X. & Chen, Z. Exosome-mediated macrophage polarization: pioneering pathways in diabetic wound healing. Int. Immunopharmacol. 161, 115058 (2025).

Google Scholar
Ilesanmi-Oyelere, B. L. et al. Inflammatory markers and bone health in postmenopausal women: a cross-sectional overview. Immun. Ageing 16, 15 (2019).

Google Scholar
Chen, H. et al. Evaluation on the corrosion resistance, antibacterial property and osteogenic activity of biodegradable Mg-Ca and Mg-Ca-Zn-Ag alloys. J. Magnes. Alloy. 10, 3380–3396 (2022).

Google Scholar
K, S., S, B., Chatterjee, S. & N, R. Titanate incorporated anodized coating on magnesium alloy for corrosion protection, antibacterial responses and osteogenic enhancement. J. Magnes. Alloy. 10, 1109–1123 (2022).

Google Scholar
Fang, H., Zhou, S., Qi, X., Wang, C. & Tian, Y. A multifunctional osteogenic system of ultrasonically spray deposited bone-active coatings on plasma-activated magnesium. J. Magnes. Alloy. 11, 2719–2739 (2023).

Google Scholar
Feroz, S., Muhammad, N., Ratnayake, J. & Dias, G. Keratin-based materials for biomedical applications. Bioact. Mater. 5, 496–509 (2020).

Google Scholar
Xie, J. et al. Developing new Mg alloy as potential bone repair material via constructing weak anode nano-lamellar structure. J. Magnes. Alloy. 11, 154–175 (2023).

Google Scholar
Sui, B., Lu, H., Liu, X. & Sun, J. High-purity Mg and Mg-1Ca alloys: comparative assessment of the merits regarding degradation, osteogenesis, and biosafety for orthopedic applications. J. Mater. Sci. Technol. 140, 58–66 (2023).

Google Scholar
Jeon, H. et al. Preparation of a nanocellulose/nanochitin coating on a poly (lactic acid) film for improved hydrolysis resistance. Int. J. Biol. Macromol. 254, 127790 (2024).

Google Scholar
Tang, H. et al. In vitro and in vivo evaluation of micro-alloyed magnesium for potential application in alveolar bone fixation screws. J. Mater. Sci. Technol. 144, 62–69 (2023).

Google Scholar
Mueller, W.-D., Lucia Nascimento, M. & Lorenzo de Mele, M. F. Critical discussion of the results from different corrosion studies of Mg and Mg alloys for biomaterial applications. Acta Biomater. 6, 1749–1755 (2010).

Google Scholar
Sun, Y. et al. Degradation particles derived from high-purity magnesium inhibit osteogenic differentiation through regulation of macrophage polarization. J. Mater. Sci. Technol. 139, 113–119 (2023).

Google Scholar
Hashemi, M., Alizadeh, R. & Langdon, T. G. Recent advances using equal-channel angular pressing to improve the properties of biodegradable Mg‒Zn alloys. J. Magnes. Alloy. 11, 2260–2284 (2023).

Google Scholar
Niranjan, C. A. et al. Magnesium alloys as extremely promising alternatives for temporary orthopedic implants—a review. J. Magnes. Alloy. 11, 2688–2718 (2023).

Google Scholar
Pan, C. et al. Bivalirudin functionalized hydrogel coating capable of catalytical NO-generation for enhanced anticorrosion and biocompatibility of magnesium alloy. Mater. Today Bio 31, 101473 (2025).

Google Scholar
Park, S.-S. et al. In-vivo bone remodeling potential of Sr-d-Ca-P /PLLA-HAp coated biodegradable ZK60 alloy bone plate. Mater. Today Bio 18, 100533 (2023).

Google Scholar
Germaini, M.-M. et al. Additive manufacturing of biomaterials for bone tissue engineering—a critical review of the state of the art and new concepts. Prog. Mater. Sci. 130, 100963 (2022).

Google Scholar
Chen, T. et al. Coupling physics in machine learning to investigate the solution behavior of binary Mg alloys. J. Magnes. Alloy. 10, 2817–2832 (2022).

Google Scholar
Liu, C. et al. Annealing hardening effect aroused by solute segregation in gradient ultrafine-grained Mg-Gd-Zr alloy. J. Mater. Sci. Technol. 144, 70–80 (2023).

Google Scholar
Feng, Z. et al. Insight into the effect of biomaterials on osteogenic differentiation of mesenchymal stem cells: A review from a mitochondrial perspective. Acta Biomater. 164, 1–14 (2023).

Google Scholar
Wolff, M. et al. Comparison between compression tested and simulated Mg-6.3Gd bone scaffolds produced by binder based additive manufacturing technique. J. Magnes. Alloy. 11, 2750–2762 (2023).

Google Scholar
Huang, Y. et al. Development and prospects of degradable magnesium alloys for structural and functional applications in the fields of environment and energy. J. Magnes. Alloy. 11, 3926–3947 (2023).

Google Scholar
Yao, Y. et al. Effect of mechanical stresses on degradation behavior of high-purity magnesium in bone environments. J. Mater. Sci. Technol. 171, 252–261 (2024).

Google Scholar
Sefa, S. et al. Multiscale morphological analysis of bone microarchitecture around Mg-10Gd implants. Bioact. Mater. 30, 154–168 (2023).

Google Scholar
Liu, G. et al. Effects of inorganic ions, organic particles, blood cells, and cyclic loading on in vitro corrosion of MgAl alloys. J. Magnes. Alloy. 11, 2429–2441 (2023).

Google Scholar
Wang, Y. et al. Corrosion behavior and mechanical property of Mg-4Li-1Ca alloys under micro-compressive stress. J. Mater. Sci. Technol. 175, 170–184 (2024).

Google Scholar
Qi, G. et al. SrHPO4-coated Mg alloy implant attenuates postoperative pain by suppressing osteoclast-induced sensory innervation in osteoporotic fractures. Mater. Today Bio 28, 101227 (2024).

Google Scholar
Wu, Y. et al. Influence of electrolyte temperature on morphology and properties of composite anodic film on titanium alloy Ti-10V-2Fe-3Al. Coatings 10, 1109 (2020).

Google Scholar
Song, G. & Atrens, A. Recent insights into the mechanism of magnesium corrosion and research suggestions. Adv. Eng. Mater. 9, 177–183 (2007).

Google Scholar
Ci, W. et al. Achieving ultra-high corrosion-resistant Mg-Zn-Sc alloys by forming Sc-assisted protective corrosion product film. J. Mater. Sci. Technol. 181, 138–151 (2024).

Google Scholar
Kiani, F. et al. Microstructures, mechanical properties, corrosion, and biocompatibility of extruded Mg-Zr-Sr-Ho alloys for biodegradable implant applications. J. Magnes. Alloy. 11, 110–136 (2023).

Google Scholar
Sun, Y., Helmholz, H. & Willumeit-Römer, R. Peri-implant gas accumulation in response to magnesium-based musculoskeletal biomaterials: reframing current evidence for preclinical research and clinical evaluation. J. Magnes. Alloy. 12, 59–71 (2024).

Google Scholar
Yang, L. et al. Influence of ageing treatment on microstructure, mechanical and bio-corrosion properties of Mg–Dy alloys. J. Mech. Behav. Biomed. Mater. 13, 36–44 (2012).

Google Scholar
Yang, L. et al. Influence of Dy in solid solution on the degradation behavior of binary Mg-Dy alloys in cell culture medium. Mater. Sci. Eng. C 75, 1351–1358 (2017).

Google Scholar
Yang, L. et al. Mechanical and corrosion properties of binary Mg–Dy alloys for medical applications. Mater. Sci. Eng. B 176, 1827–1834 (2011).

Google Scholar
Kiani, F., Lin, J., Munir, K., Wen, C. & Li, Y. Improvements in mechanical, corrosion, and biocompatibility properties of Mg–Zr–Sr–Dy alloys via extrusion for biodegradable implant applications. J. Magnes. Alloy. 11, 3840–3865 (2023).

Google Scholar
Bi, G. et al. Microstructure and mechanical properties of an extruded Mg-Dy-Ni alloy. Mater. Sci. Eng. A 760, 246–257 (2019).

Google Scholar
Li, D. et al. In vitro and in vivo assessment of the effect of biodegradable magnesium alloys on osteogenesis. Acta Biomater. 141, 454–465 (2022).

Google Scholar
Chen, J. S. et al. Formation mechanism of W phase and its effects on the mechanical properties of Mg−Dy−Zn alloys. J. Magnes. Alloy. 13, 2174–2189 (2025).

Google Scholar
Munir, K., Lin, J., Wen, C., Wright, P. F. A. & Li, Y. Mechanical, corrosion, and biocompatibility properties of Mg-Zr-Sr-Sc alloys for biodegradable implant applications. Acta Biomater. 102, 493–507 (2020).

Google Scholar
Kiani, F., Wen, C. & Li, Y. Prospects and strategies for magnesium alloys as biodegradable implants from crystalline to bulk metallic glasses and composites—a review. Acta Biomater. 103, 1–23 (2020).

Google Scholar
Shi, H. et al. Microstructural evolution of Mg–14Gd–0.4Zr alloy during compressive creep. J. Magnes. Alloy. 11, 3161–3173 (2023).

Google Scholar
Guo, T., Siska, F. & Barnett, M. R. Distinguishing between slip and twinning events during nanoindentation of magnesium alloy AZ31. Scr. Mater. 110, 10–13 (2016).

Google Scholar
Guo, T., Siska, F., Cheng, J. & Barnett, M. Initiation of basal slip and tensile twinning in magnesium alloys during nanoindentation. J. Alloy. Compd. 731, 620–630 (2018).

Google Scholar
Ambrosi, T. H. et al. Aged skeletal stem cells generate an inflammatory degenerative niche. Nature 597, 256–262 (2021).

Google Scholar
Pajarinen, J. et al. Mesenchymal stem cell-macrophage crosstalk and bone healing. Biomaterials 196, 80–89 (2019).

Google Scholar
Chen, X. et al. Enhancing the mechanical properties and osteogenic differentiation of Mg-1 wt.%Ho alloy by tailoring microstructures through extrusion temperatures. J. Mater. Res. Technol. 30, 6941–6952 (2024).

Google Scholar
Wang, W. et al. High-performance Mg–Zn alloy achieved by the ultrafine grain and nanoparticle design. Bioact. Mater. 41, 371–384 (2024).

Google Scholar
Zhang, Z. et al. In vitro and in vivo evaluation of osteogenesis and antibacterial activity of MgGa alloys. Acta Biomater. 185, 85–97 (2024).

Google Scholar
Liu, J. et al. Comparative in vitro study on binary Mg-RE (Sc, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu) alloy systems. Acta Biomater. 102, 508–528 (2020).

Google Scholar
Ding, Y., Lin, J., Wen, C., Zhang, D. & Li, Y. Mechanical properties, corrosion, and biocompatibility of Mg-Zr-Sr-Dy alloys for biodegradable implant applications. J. Biomed. Mater. Res. B. Appl. Biomater. 106, 2425–2434 (2018).

Google Scholar
Feyerabend, F. et al. Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines. Acta Biomater. 6, 1834–1842 (2010).

Google Scholar
Gao, J., Feng, L., Chen, B., Fu, B. & Zhu, M. The role of rare earth elements in bone tissue engineering scaffolds—a review. Compos. Part B Eng. 235, 109758 (2022).

Google Scholar
Guo, A. et al. A β-Si3N4/HA composite materials with biomimetic mineralized CaCO3 coating promote angiogenesis and bone regeneration through immunomodulation. Biomater. Adv. 181, 214629 (2026).

Google Scholar
Lou, P. et al. Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis. Bone Res. 13, 94 (2025).

Google Scholar
Jiménez-Ortega, R. F. et al. Impact of the dietary antioxidant index on bone mineral density gain among mexican adults: a prospective study. Arch. Osteoporos. 20, 38 (2025).

Google Scholar
Li, N. & Guo, X. The gut microbiota and host immunity synergistically orchestrate colonization resistance. Gut Microbes 18, 2611545 (2026).

Google Scholar
Durairajan, S. S. K. et al. Molecular links between inflammatory bowel disease and Alzheimer’s disease through immune signaling and inflammatory pathways. World J. Gastroenterol. 31, 111301 (2025).

Google Scholar
Zhang, X. et al. Negatively correlated corrosion and stress corrosion cracking of Mg-Al-Mn-Ca based alloys. Corros. Sci. 227, 111747 (2024).

Google Scholar

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