Embryonic nano lauric acid delivery modulates lipid metabolism, oxidative balance, and gut morphogenesis in broiler chicks

embryonic-nano-lauric-acid-delivery-modulates-lipid-metabolism,-oxidative-balance,-and-gut-morphogenesis-in-broiler-chicks
Embryonic nano lauric acid delivery modulates lipid metabolism, oxidative balance, and gut morphogenesis in broiler chicks

References

  1. El-Shater, S. N. et al. Effect of in-ovo inoculation of betaine on hatchability, serum antioxidant levels, muscle gene expression and intestinal development of broiler chicks. J. Anim. Physiol. Anim. Nutr. 108, 883–890 (2024).

    Google Scholar 

  2. Mousstaaid, A. et al. Effects of the in ovo and dietary supplementation of L-ascorbic acid on the growth performance, inflammatory response, and eye L-ascorbic acid concentrations in Ross 708 broiler chickens. Animals 12, 2573 (2022).

    Google Scholar 

  3. Qiu, C. et al. Stabilisation of oleofoams by lauric acid and its glycerol esters. Food Chem. 386, 132776 (2022).

    Google Scholar 

  4. Singh, A. R. et al. Nanotechnology-based approaches applied to nutraceuticals. Drug Deliv. Transl. Res. 12, 485–499 (2022).

    Google Scholar 

  5. Bai, K. et al. Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poult. Sci. 96, 74–82 (2017).

    Google Scholar 

  6. Ngo, V. & Duennwald, M. L. Nrf2 and oxidative stress: A general overview of mechanisms and implications in human disease. Antioxidants 11, 2345 (2022).

    Google Scholar 

  7. Werner, H. The IGF1 signaling pathway: From basic concepts to therapeutic opportunities. Int. J. Mol. Sci. 24, 14882 (2023).

    Google Scholar 

  8. Hosnedlova, B. et al. Associations between IGF1, IGFBP2 and TGFß3 genes polymorphisms and growth performance of broiler chicken lines. Animals 10, 800 (2020).

    Google Scholar 

  9. Alvarenga, R., Zangeronimo, M., Pereira, L., Rodrigues, P. & Gomide, E. Lipoprotein metabolism in poultry. Worlds Poult. Sci. J. 67, 431–440 (2011).

    Google Scholar 

  10. de Paula, K. L. C. et al. Sources of conjugated linoleic acid and lauric acid inoculated into the eggs of quails and its effects on immunity. Semina: Ciências Agrárias 42, 1759–1772 (2021).

    Google Scholar 

  11. Givisiez, P. E. et al. Chicken embryo development: metabolic and morphological basis for in ovo feeding technology. Poult. Sci. 99, 6774–6782 (2020).

    Google Scholar 

  12. Kong, L., Cai, Y., Pan, X., Xiao, C. & Song, Z. Glycerol monolaurate improves intestinal morphology and antioxidant status by suppressing inflammatory responses and nuclear factor kappa-B signaling in lipopolysaccharide-exposed chicken embryos. Anim. Nutr. 15, 297–306 (2023).

    Google Scholar 

  13. Sharma, J. Embryo vaccination with infectious bursal disease virus alone or in combination with Marek’s disease vaccine. Avian Dis. 29, 1155–1169 (1985).

    Google Scholar 

  14. Uni, Z., Ferket, P., Tako, E. & Kedar, O. In ovo feeding improves energy status of late-term chicken embryos. Poult. Sci. 84, 764–770 (2005).

    Google Scholar 

  15. Schijns, V.E., van de Zande, S., Lupiani, B. & Reddy, S.M. Practical aspects of poultry vaccination. In Avian Immunology 345–362 (Elsevier, 2014).

  16. Bednarczyk, M., Dunislawska, A., Stadnicka, K. & Grochowska, E. Chicken embryo as a model in epigenetic research. Poult. Sci. 100, 101164 (2021).

    Google Scholar 

  17. Das, R., Mishra, P. & Jha, R. In ovo feeding as a tool for improving performance and gut health of poultry: A review. Front. Vet. Sci. 8, 754246 (2021).

    Google Scholar 

  18. Abdel-Moneim, A.-M.E., Elbaz, A. M., Khidr, R.E.-S. & Badri, F. B. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob. Prot. 12, 873–882 (2020).

    Google Scholar 

  19. Niknafs, S., Meijer, M. M., Khaskheli, A. A. & Roura, E. In ovo delivery of oregano essential oil activated xenobiotic detoxification and lipid metabolism at hatch in broiler chickens. Poult. Sci. 103, 103321 (2024).

    Google Scholar 

  20. Hosseintabar-Ghasemabad, B. et al. Applications and impacts of nanotechnology in poultry nutrition. Discov. Appl. Sci. 7, 938 (2025).

    Google Scholar 

  21. Lima, R. D. S. & Block, J. M. Coconut oil: What do we really know about it so far?. Food Qual. Saf. 3, 61–72 (2019).

    Google Scholar 

  22. Huang, L., Gao, L. & Chen, C. Role of medium-chain fatty acids in healthy metabolism: A clinical perspective. Trends Endocrinol. Metab. 32, 351–366 (2021).

    Google Scholar 

  23. Hadrová, S., Sedláková, K., Křížová, L. & Malyugina, S. Alternative and unconventional feeds in dairy diets and their effect on fatty acid profile and health properties of milk fat. Animals 11, 1817 (2021).

    Google Scholar 

  24. Jackman, J. A., Yoon, B. K., Li, D. & Cho, N.-J. Nanotechnology formulations for antibacterial free fatty acids and monoglycerides. Molecules 21, 305 (2016).

    Google Scholar 

  25. Abd El-Ghany, W. A., Shaalan, M. & Salem, H. M. Nanoparticles applications in poultry production: An updated review. World’s Poult. Sci. J. 77, 1001–1025 (2021).

    Google Scholar 

  26. Sadr, S. et al. Nanotechnology innovations for increasing the productivity of poultry and the prospective of nanobiosensors. Vet. Med. Sci. 9, 2118–2131 (2023).

    Google Scholar 

  27. Ahmed, M. M., Ismail, Z. S., Elwardany, I., Lohakare, J. & Abdel-Wareth, A. A. In Ovo feeding techniques of green nanoparticles of silver and probiotics: Evaluation of performance, physiological, and microbiological responses of hatched one-day-old broiler chicks. Animals 13, 3725 (2023).

    Google Scholar 

  28. Dayrit, F. M. The properties of lauric acid and their significance in coconut oil. J. Am. Oil. Chem. Soc. 92, 1–15 (2015).

    Google Scholar 

  29. Koppenol, A. et al. Effect of the ratio of dietary n-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid on broiler breeder performance, egg quality, and yolk fatty acid composition at different breeder ages. Poult. Sci. 93, 564–573 (2014).

    Google Scholar 

  30. Fontinha, F., Martins, N., Magalhães, R., Peres, H. & Oliva-Teles, A. Dietary lauric acid supplementation positively affects growth performance, oxidative and immune status of European Seabass Juveniles. Fishes 10, 190 (2025).

    Google Scholar 

  31. Xu, W. et al. Effects of in ovo feeding of t10, c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks. Poult. Sci. 101, 101797 (2022).

    Google Scholar 

  32. Xu, X. et al. Glycerol monolaurate enhances growth performance, lipid metabolism, and inflammatory response in common carp fed high lipid diets. Fish Shellfish Immunol. 155, 109988 (2024).

    Google Scholar 

  33. Zhan, W. et al. Dietary lauric acid promoted antioxidant and immune capacity by improving intestinal structure and microbial population of swimming crab (Portunus trituberculatus). Fish Shellfish Immunol. 151, 109739 (2024).

    Google Scholar 

  34. Li, H. et al. Medium-chain fatty acids decrease serum cholesterol via reduction of intestinal bile acid reabsorption in C57BL/6J mice. Nutr. Metab. 15, 37 (2018).

    Google Scholar 

  35. Lekshmi Sheela, D., Nazeem, P. A., Narayanankutty, A., Manalil, J. J. & Raghavamenon, A. C. In silico and wet lab studies reveal the cholesterol-lowering efficacy of lauric acid, a medium-chain fat of coconut oil. Plant Foods Hum. Nutr. 71, 410–415 (2016).

    Google Scholar 

  36. Maki, K. C. et al. Corn oil lowers plasma cholesterol compared with coconut oil in adults with above-desirable levels of cholesterol in a randomized crossover trial. J. Nutr. 148, 1556–1563 (2018).

    Google Scholar 

  37. Zhang, F. et al. Dietary supplementation of lauric acid alleviates the irregular estrous cycle and the impaired metabolism and thermogenesis in female mice fed with a high-fat diet (HFD). J. Agric. Food Chem. 68, 12631–12640 (2020).

    Google Scholar 

  38. Elewa, M. S. et al. Effect of coconut oil on growth performance, carcass criteria, liver and kidney functions, antioxidants and immunity, and lipid profile of broilers. Sci. Rep. 13, 13974 (2023).

    Google Scholar 

  39. Attia, Y. A., Al-Harthi, M. A. & Abo El-Maaty, H. M. The effects of different oil sources on performance, digestive enzymes, carcass traits, biochemical, immunological, antioxidant, and morphometric responses of broiler chicks. Front. Vet. Sci. 7, 181 (2020).

    Google Scholar 

  40. Saeidi, E., Shokrollahi, B., Karimi, K. & Amiri-Andi, M. Effects of medium-chain fatty acids on performance, carcass characteristics, blood biochemical parameters and immune response in Japanese quail. Br. Poult. Sci. 57, 358–363 (2016).

    Google Scholar 

  41. Ullah, S., Feng, F., Zhao, M., Zhang, J. & Shao, Q. Effect of dietary supplementation of lauric acid on growth performance, digestive enzymes, serum immune and antioxidant parameters, and intestinal morphology in black sea bream. Fish Physiol. Biochem. 51, 43 (2025).

    Google Scholar 

  42. Ramya, V., Shyam, K. P., Kowsalya, E., Balavigneswaran, C. K. & Kadalmani, B. Dual roles of coconut oil and its major component lauric acid on redox nexus: Focus on cytoprotection and cancer cell death. Front. Neurosci. 16, 833630 (2022).

    Google Scholar 

  43. Hewlings, S. Coconuts and health: Different chain lengths of saturated fats require different consideration. J. Cardiovasc. Dev. Dis. 7, 59 (2020).

    Google Scholar 

  44. Alfhili, M. A. & Aljuraiban, G. S. Lauric acid, a dietary saturated medium-chain fatty acid, elicits calcium-dependent eryptosis. Cells 10, 3388 (2021).

    Google Scholar 

  45. Yang, B., Dong, Y., Wang, F. & Zhang, Y. Nanoformulations to enhance the bioavailability and physiological functions of polyphenols. Molecules 25, 4613 (2020).

    Google Scholar 

  46. Chowdhury, S. & Saikia, S. Oxidative stress in fish: A review. J. Sci. Res. 12, 145–160 (2020).

    Google Scholar 

  47. Anuar, N. S. et al. Lauric acid improves hormonal profiles, antioxidant properties, sperm quality and histomorphometric changes in testis and epididymis of streptozotocin-induced diabetic infertility rats. Toxicol. Appl. Pharmacol. 470, 116558 (2023).

    Google Scholar 

  48. Zheng, M. et al. The applications and mechanisms of superoxide dismutase in medicine, food, and cosmetics. Antioxidants 12, 1675 (2023).

    Google Scholar 

  49. Surai, P. F., Fisinin, V. I. & Karadas, F. Antioxidant systems in chick embryo development. Part 1. Vitamin E, carotenoids and selenium. Anim. Nutr. 2, 1–11 (2016).

    Google Scholar 

  50. He, F., Ru, X. & Wen, T. NRF2, a transcription factor for stress response and beyond. Int. J. Mol. Sci. 21, 4777 (2020).

    Google Scholar 

  51. Wang, D., Chen, J., Sun, H., Chen, W. & Yang, X. MCFA alleviates H2O2-induced oxidative stress in AML12 cells via the ERK1/2/Nrf2 pathway. Lipids 57, 153–162 (2022).

    Google Scholar 

  52. Kong, L., Wang, Z., Xiao, C., Zhu, Q. & Song, Z. Glycerol monolaurate attenuated immunological stress and intestinal mucosal injury by regulating the gut microbiota and activating AMPK/Nrf2 signaling pathway in lipopolysaccharide-challenged broilers. Anim. Nutr. 10, 347–359 (2022).

    Google Scholar 

  53. Wang, Q. et al. Effects of dietary supplementation of glycerol monolaurate on laying performance, egg quality, antioxidant capacity, intestinal morphology and immune function in late-phase laying hens. Poult. Sci. 103, 103644 (2024).

    Google Scholar 

  54. Kong, L., Wang, Z., Xiao, C., Zhu, Q. & Song, Z. Glycerol monolaurate ameliorated intestinal barrier and immunity in broilers by regulating intestinal inflammation, antioxidant balance, and intestinal microbiota. Front. Immunol. 12, 713485 (2021).

    Google Scholar 

  55. Ullah, S. et al. Effect of dietary supplementation of lauric acid on growth performance, antioxidative capacity, intestinal development and gut microbiota on black sea bream (Acanthopagrus schlegelii). PLoS ONE 17, e0262427 (2022).

    Google Scholar 

  56. Simó-Mirabet, P. et al. Sodium salt medium-chain fatty acids and Bacillus-based probiotic strategies to improve growth and intestinal health of gilthead sea bream (Sparus aurata). PeerJ 5, e4001 (2017).

    Google Scholar 

  57. Onwuka, O. M. & Ige, A. O. Lauric acid treatment alleviates type-II-diabetes-induced osteoarthritis by activating joint Nrf2/HO-1 pathways resulting in enhanced synovial antioxidant activity and reduced inflammation. Egypt. J. Basic Appl. Sci. 12, 220–236 (2025).

    Google Scholar 

  58. Shaheryar, Z. A. et al. Lauric acid provides neuroprotection against oxidative stress in mouse model of hyperglycaemic stroke. Eur. J. Pharmacol. 956, 175990 (2023).

    Google Scholar 

  59. Jiang, H. et al. Malic enzyme 3 mediated the effects of malic acid on intestinal redox status and feed efficiency in broilers. J. Anim. Sci. Biotechnol. 16, 28 (2025).

    Google Scholar 

  60. Hyun, S. Body size regulation and insulin-like growth factor signaling. Cell. Mol. Life Sci. 70, 2351–2365 (2013).

    Google Scholar 

  61. Bełdowska, A. et al. The effect of sodium butyrate administered in ovo on the health status and intestinal response in broiler chicken. Poult. Sci. 103, 104108 (2024).

    Google Scholar 

  62. Watanabe, S. & Tsujino, S. Applications of medium-chain triglycerides in foods. Front. Nutr. 9, 802805 (2022).

    Google Scholar 

  63. Fujita, S. et al. Role of insulin-like growth factor-1 in the central regulation of feeding behavior in chicks. J. Poult. Sci. 56, 270–276 (2019).

    Google Scholar 

  64. Zhao, C. et al. IGF2 promotes the differentiation of chicken embryonic myoblasts by regulating mitochondrial remodeling. J. Cell. Physiol. 239, e31351 (2024).

    Google Scholar 

  65. Beatty, A. E. & Schwartz, T. S. Gene expression of the IGF hormones and IGF binding proteins across time and tissues in a model reptile. Physiol. Genomics 52, 423–434 (2020).

    Google Scholar 

  66. Liu, H. et al. Biological function of medium-chain fatty acids and their application in aquatic animals: A review. Animals 15, 2294 (2025).

    Google Scholar 

  67. Gangadoo, S., Stanley, D., Hughes, R. J., Moore, R. J. & Chapman, J. Nanoparticles in feed: Progress and prospects in poultry research. Trends Food Sci. Technol. 58, 115–126 (2016).

    Google Scholar 

  68. Gomez-Osorio, L.-M., Yepes-Medina, V., Ballou, A., Parini, M. & Angel, R. Short and medium chain fatty acids and their derivatives as a natural strategy in the control of necrotic enteritis and microbial homeostasis in broiler chickens. Front. Vet. Sci. 8, 773372 (2021).

    Google Scholar 

  69. Meng, Z., Tan, D., Cheng, Z., Jiang, M. & Zhan, K. GPR41 regulates the proliferation of BRECs via the PIK3-AKT-mTOR pathway. Int. J. Mol. Sci. 24, 4203 (2023).

    Google Scholar 

  70. Baltić, B. et al. Effect of dietary supplementation with medium-chain fatty acids on growth performance, intestinal histomorphology, lipid profile, and intestinal microflora of broiler chickens. S. Afr. J. Anim. Sci. 48, 885–896 (2018).

    Google Scholar 

  71. Arifin, W. N. & Zahiruddin, W. M. Sample size calculation in animal studies using resource equation approach. Malays. J. Med. Sci. MJMS 24, 101 (2017).

    Google Scholar 

  72. Oke, O. et al. In ovo injection of black cumin (Nigella sativa) extract on hatching and post-hatch performance of thermally challenged broiler chickens during incubation. Poult. Sci. 100, 100831 (2021).

    Google Scholar 

  73. Abdel-Moneim, A.-M.E., Elbaz, A. M., Khidr, R.E.-S. & Badri, F. B. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob. Prot. 12, 873–882 (2020).

    Google Scholar 

  74. Cook, M., Scott, T., Hebert, J. & Johnson, W. Effects of blood sampling by cardiac puncture on subsequent body weights of broilers. Poult. Sci. 61, 2506–2508 (1982).

    Google Scholar 

  75. Fayed, R. H., Ali, S. E., Yassin, A. M., Madian, K. & Bawish, B. M. Terminalia bellirica and Andrographis paniculata dietary supplementation in mitigating heat stress-induced behavioral, metabolic, and genetic alterations in broiler chickens. BMC Vet. Res. 20, 388 (2024).

    Google Scholar 

  76. Lopes-Virella, M. F., Stone, P., Ellis, S. & Colwell, J. A. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin. Chem. 23, 882–884 (1977).

    Google Scholar 

  77. Richmond, W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin. Chem. 19, 1350–1356 (1973).

    Google Scholar 

  78. Tietz, N. W. Clinical guide to laboratory tests. In Clinical Guide to Laboratory Tests 1096–1096 (1995).

  79. Friedewald, W. T., Levy, R. I. & Fredrickson, D. S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499–502 (1972).

    Google Scholar 

  80. Mousa, Y., Amin, S. & Shaaban, K. A. Pharmacokinetics and plasma concentration of thiopental in normal and stressed chickens with hydrogen peroxide. J. Hellenic Vet. Med. Soc. 72, 2961–2968 (2021).

    Google Scholar 

  81. Alaa, M. et al. Guanidinoacetic acid supplementation and stocking density effects on broiler performance: Behavior, biochemistry, immunity, and small intestinal histomorphology. Acta Vet. Scand. 66, 62 (2024).

    Google Scholar 

  82. Ohkawa, H., Ohishi, N. & Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351–358 (1979).

    Google Scholar 

  83. Beutler, E., Duron, O. & Kelly, B.M. Improved method for determination of blood glutathione. (1963).

  84. Abdelfatah, S. H., Yassin, A. M., Khattab, M. S., Abdel-Razek, A. S. & Saad, A. H. Spirulina platensis as a growth booster for broiler; Insights into their nutritional, molecular, immunohistopathological, and microbiota modulating effects. BMC Vet. Res. 20, 11 (2024).

    Google Scholar 

  85. Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative CT method. Nat. Protoc. 3, 1101–1108 (2008).

    Google Scholar 

  86. Bancroft, J. D. & Gamble, M. Theory and Practice of Histological Techniques (Elsevier, 2008).

    Google Scholar 

  87. Team, P. RStudio: Integrated Development Environment for R; Posit Software, PBC: Boston (2023).

  88. Shapiro, S. S. & Wilk, M. B. An analysis of variance test for normality (complete samples). Biometrika 52, 591–611 (1965).

    Google Scholar 

  89. Levene, H. Robust tests for equality of variances. In Contributions to Probability and Statistics, 278–292 (1960).

  90. Benjamini, Y. & Hochberg, Y. Controlling the false discovery rate: A practical and powerful approach to multiple testing. J. Roy. Stat. Soc.: Ser. B (Methodol.) 57, 289–300 (1995).

    Google Scholar 

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