Multiplex real-time PCR with high-resolution melting analysis for rapid identification of carbapenem and colistin resistance genes in clinical Enterobacterales isolates

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Authors and Affiliations

1. Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand

   Sirirat Luk-In, Kamonrat Phopin, Sasina Bangmuangngam & Ratana Lawung

2. Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand

   Kamonrat Phopin & Tanawut Tantimongcolwat

3. Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

   Tanittha Chatsuwan & Aye Mya Sithu Shein

4. Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

   Tanittha Chatsuwan & Aye Mya Sithu Shein

5. Department of Infectious Diseases, Faculty of Medicine, Yamagata University, Yamagata, Japan

   Dhammika Leshan Wannigama

6. Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan

   Dhammika Leshan Wannigama

7. Pathogen Hunter’s Research Collaborative Team, Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan

   Dhammika Leshan Wannigama

8. Yamagata Prefectural University of Health Sciences, Kamiyanagi, Yamagata, 990-2212, Japan

   Dhammika Leshan Wannigama

9. Biofilms and Antimicrobial Resistance Consortium of ODA Receiving Countries, The University of Sheffield, Sheffield, UK

   Dhammika Leshan Wannigama

Contributions

S.L.: conceptualisation, funding acquisition, methodology, data curation, data analysis, statistical analysis, supervision, validation, writing a draft manuscript, editing, and finalising the manuscript. K.P.: conceptualisation, funding acquisition, project administration, supervision, validation, critical review, editing, and finalising the manuscript. S.B.: methodology, data curation, editing, and finalising the manuscript. T.C., D.L.W., A.M.S.S., R.L.: data curation, supervision, validation, critical review, editing, and finalising the manuscript. T.T.: conceptualisation, funding acquisition, project administration, supervision, validation, critical review, editing, and finalising the manuscript. All the authors have read, reviewed, and approved this published version.

Corresponding author

Correspondence to Tanawut Tantimongcolwat.

Competing Interests

The authors declare no competing interests.

Ethical approval

The study was conducted in accordance with biosafety guidelines and approved by the Mahidol University-Institutional Biosafety Committee (MU-IBC), Mahidol University (Nakhon Pathom, Thailand) [MU 2023–030 and MU 2024–021]. This study of anonymised clinical isolates did not include interaction or intervention with human subjects or access to identifiable private information. The Mahidol University Central Institutional Review Board (MU-CIRB), Mahidol University (Nakhon Pathom, Thailand), waived the requirement for IRB review.

Informed consent

For this retrospective study of anonymous clinical isolates, the requirement for informed consent from patients was waived by the Mahidol University Central Institutional Review Board (MU-CIRB), Mahidol University (Nakhon Pathom, Thailand).

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