References
-
Joseph, J. & Peter, C. Chromolaena odorata extracts as a bio-organic treatment for downy mildew (Peronospora belbahrii) in basil (Ocimum basilicum). Malays J. Invent. Innov. 3, 1–8. https://doi.org/10.5281/zenodo.10924595 (2024).
-
Johnson, E. T. et al. Dual transcriptional analysis of Ocimum basilicum and Peronospora belbahrii in susceptible interactions. Plant. Gene. 29, 100350. https://doi.org/10.1016/j.plgene.2021.100350 (2022).
-
Yadav, S. S., Suryavanshi, P., Nishad, I. & Sinha, S. First report of downy mildew on sweet basil caused by Peronospora belbahrii in India. Plant. Dis. 106, 318. https://doi.org/10.1094/PDIS-03-21-0621-PDN (2022).
-
Falach-Block, L., Ben-Naim, Y. & Cohen, Y. Investigation of seed transmission in Peronospora belbahrii the causal agent of basil downy mildew. Agronomy 9, 205. https://doi.org/10.3390/agronomy9040205 (2019).
-
Silva, A. L., Mansur, P. S. C., Barreto, R. W. & Salcedo-Sarmiento, S. Peronospora belbahrii causes downy mildew on Ocimum basilicum in Brazil. Australas Plant. Dis. Notes. 14, 1–3. https://doi.org/10.1007/s13314-019-0368-z (2019).
-
Salcedo, A. F. et al. Fantastic downy mildew pathogens and how to find them: Advances in detection and diagnostics. Plants 10, 435. https://doi.org/10.3390/plants10030435 (2021).
-
Wyenandt, C. A. et al. Basil downy mildew (Peronospora belbahrii): Discoveries and challenges relative to its control. Phytopathology 105, 885–894. https://doi.org/10.1094/PHYTO-02-15-0032-FI (2015).
-
Standish, J. R., Raid, R. N., Pigg, S. & Quesada-Ocampo, L. M. A diagnostic guide for basil downy mildew. Plant. Health Prog. 21, 77–81. https://doi.org/10.1094/PHP-09-19-0062-DG (2020).
-
Yu, L. Z. et al. A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode. Eur. J. Plant. Pathol. 150, 725–734. https://doi.org/10.1007/s10658-017-1320-8 (2018).
-
Guarnaccia, V., Gilardi, G., Napoletano, E., Garibaldi, A. & Gullino, M. L. A new foliar disease of sweet basil caused by Stagonosporopsis vannaccii. J. Plant. Pathol. 104, 1491–1498. https://doi.org/10.1007/s42161-022-01197-w (2022).
-
Ghebrial, E. & Nada, M. Suppression of basil downy mildew caused by Peronospora belbahrii using resistance inducers, mineral salts and anti-transpirants combined with different rates of nitrogen fertilizer under field conditions. Egypt. J. Phytopathol. 45, 71–97. https://doi.org/10.21608/ejp.2017.89724 (2017).
-
Koroch, A. R., Villani, T. S., Pyne, R. M. & Simon, J. E. Rapid staining method to detect and identify downy mildew (Peronospora belbahrii) in basil. Appl. Plant. Sci. 1, 1300032. https://doi.org/10.3732/apps.1300032 (2013).
-
Withers, S. et al. Using next-generation sequencing to develop molecular diagnostics for Pseudoperonospora cubensis, the cucurbit downy mildew pathogen. Phytopathology 106, 1105–1116. https://doi.org/10.1094/PHYTO-10-15-0260-FI (2016).
-
Klein, J. et al. Genome reconstruction of the non-culturable spinach downy mildew Peronospora effusa by metagenome filtering. PLoS One. 15, e0225808. https://doi.org/10.1371/journal.pone.0225808 (2020).
-
Wang, T. et al. Molecular diagnostics and detection of oomycetes on fiber crops. Plants 9, 769. https://doi.org/10.3390/plants9060769 (2020).
-
Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, E63. https://doi.org/10.1093/nar/28.12.e63 (2000).
-
Hudson, O. et al. Detection of Phytophthora capsici in irrigation water using loop-mediated isothermal amplification. J. Vis. Exp. 160, e61478. https://doi.org/10.3791/61478 (2020).
-
Das, D., Lin, C. W. & Chuang, H. S. LAMP-based point-of-care biosensors for rapid pathogen detection. Biosensors 12, 1068. https://doi.org/10.3390/bios12121068 (2022).
-
Akhmetzianova, L. U. et al. LAMPrimers iQ: New primer design software for loop-mediated isothermal amplification (LAMP). Anal. Biochem. 684, 115376. https://doi.org/10.1016/j.ab.2023.115376 (2024).
-
Drais, M. I. et al. Development and validation of a loop-mediated isothermal amplification technique (LAMP) for the detection of Spiroplasma citri, the causal agent of citrus stubborn disease. Eur. J. Plant. Pathol. 155, 125–134. https://doi.org/10.1007/s10658-019-01755-6 (2019).
-
Oscorbin, I. P. et al. PI primers increase the efficacy of LAMP and RT-LAMP for SARS-CoV-2 and MS2 phage detection. Diagn. Microbiol. Infect. Dis. 110, 116449. https://doi.org/10.1016/j.diagmicrobio.2024.116449 (2024).
-
Dyussembayev, K. et al. Biosensor technologies for early detection and quantification of plant pathogens. Front. Chem. 9, 636245. https://doi.org/10.3389/fchem.2021.636245 (2021).
-
Bano, H. & Khan, J. A. Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viruses infecting patchouli (Pogostemon cablin). Arch. Microbiol. 206, 1–8. https://doi.org/10.1007/s00203-023-03798-0 (2024).
-
Kant, P. et al. Development and application of a loop-mediated isothermal amplification (LAMP) assay for the detection of Pseudomonas syringae pathovars pisi and syringae. Agriculture 11, 875. https://doi.org/10.3390/agriculture11090875 (2021).
-
Achari, S. R., Mann, R. C., Sharma, M. & Edwards, J. Diagnosis of Fusarium oxysporum f. sp. ciceris causing Fusarium wilt of chickpea using loop-mediated isothermal amplification (LAMP) and conventional end-point PCR. Sci. Rep. 13, 1–10. https://doi.org/10.1038/s41598-023-29730-6 (2023).
-
Ahuja, A. et al. Rapid and sensitive detection of potato cyst nematode Globodera rostochiensis by loop-mediated isothermal amplification assay. 3 Biotech. 11, 294. https://doi.org/10.1007/s13205-021-02830-8 (2021).
-
Hieno, A. et al. Detection of the genus Phytophthora and the species Phytophthora nicotianae by LAMP with a QProbe. Plant. Dis. 104, 2469–2480. https://doi.org/10.1094/PDIS-12-19-2523-RE (2020).
-
Feng, H. et al. Development of LAMP assays using a novel target gene for specific detection of Pythium terrestris, Pythium spinosum, and Candidatus Pythium huanghuaiense. Plant. Dis. 105, 2888–2897. https://doi.org/10.1094/PDIS-01-21-0068-RE (2021).
-
Søndreli, K. L., Tabima, J. F. & LeBoldus, J. M. Rapid new diagnostic LAMP (loop-mediated isothermal amplification) assays to distinguish among the four lineages of Phytophthora ramorum. Plant. Dis. 107, 3553–3559. https://doi.org/10.1094/PDIS-08-22-1965-RE (2023).
-
Farmer, A. A., Brierley, J. L., Lynott, J. S. & Lees, A. K. A loop-mediated isothermal amplification (LAMP) assay for the detection of Bremia lactucae in the field. Plant. Dis. 108, 2771–2777. https://doi.org/10.1094/PDIS-10-23-2001-RE (2024).
-
Lee, I. S., Kim, W., Jo, G. & Yang, K. Y. Rapid detection of a downy mildew pathogen, Peronospora destructor, in infected onion tissues and soils by loop-mediated isothermal amplification. Phytopathology 114, 1237–1243. https://doi.org/10.1094/PHYTO-11-23-0440-R (2024).
-
Saitou, N. & Nei, M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454 (1987).
-
Tamura, K., Stecher, G. & Kumar, S. MEGA11: Molecular evolutionary genetics analysis version 11. Mol. Biol. Evol. 38, 3022–3027. https://doi.org/10.1093/molbev/msab120 (2021).
-
Pushpavathi, B. et al. Emergence of basil downy mildew in India: Need more domestic vigilance to combat spread of Peronospora belbahrii. Vegetos 38, 408–411. https://doi.org/10.1007/s42535-024-01031-x (2025).
-
Topolovec-Pintarić, S. & Martinko, K. Downy mildew of basil: A new plant disease-current threats and management trends. In Plant Diseases: Current Threats and Management Trends 3–19 (IntechOpen, 2020). https://doi.org/10.5772/intechopen.80762.
-
Chen, D. Z. et al. Development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Pseudomonas syringae pv. tomato in plant. Eur. J. Plant. Pathol. 156, 739–750. https://doi.org/10.1007/s10658-019-01923-8 (2020).
-
Keizerweerd, A. T., Chandra, A. & Grisham, M. P. Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane. J. Virol. Methods. 212, 23–29. https://doi.org/10.1016/j.jviromet.2014.10.013 (2015).
-
Muttappagol, M. et al. Multilocus sequence analysis of Candidatus Phytoplasma asteris associated with phyllody of cucumber in India and development of loop-mediated isothermal amplification (LAMP) assay for its detection. Physiol. Mol. Plant. Pathol. 133, 102350. https://doi.org/10.1016/j.pmpp.2024.102350 (2024).
-
Cassedy, A., Mullins, E. & O’Kennedy, R. Sowing seeds for the future: The need for on-site plant diagnostics. Biotechnol. Adv. 39, 107358. https://doi.org/10.1016/j.biotechadv.2019.02.014 (2020).
-
Méndez Tibambre, M. E. et al. Método LAMP como alternativa diagnostica para la detección del virus SARS-CoV-2. Rev. Colomb Cienc. Quím Farm. 50, 633–649. https://doi.org/10.15446/rcciquifa.v50n3.10020 (2021).
-
García-Chávez, M. D. L. et al. Á. Detection of Impatiens necrotic spot virus (INSV) in thrips and ornamental plants by RT-LAMP. Biotecnia 26. https://doi.org/10.18633/biotecnia.v26.2256 (2024).
-
Sui, X., Zhang, S., Wu, Z. & Ling, K. S. Reverse transcription loop-mediated isothermal amplification for species-specific detection of tomato chlorotic spot orthotospovirus. J. Virol. Methods. 253, 56–60. https://doi.org/10.1016/j.jviromet.2018.01.002 (2018).
-
Kong, X. et al. Development and application of loop-mediated isothermal amplification (LAMP) for detection of Plasmopara viticola. Sci. Rep. 6, 28935. https://doi.org/10.1038/srep28935 (2016).
-
Lu, C., Song, B., Zhang, H., Wang, Y. & Zheng, X. Rapid diagnosis of soybean seedling blight caused by Rhizoctonia solani and soybean charcoal rot caused by Macrophomina phaseolina using LAMP assays. Phytopathology 105, 1612–1617. https://doi.org/10.1094/PHYTO-01-15-0023-R (2015).
-
Koike, S. T., Stanghellini, H. & Burkhardt, A. First report of macrophomina crown rot caused by Macrophomina phaseolina on basil in the United States. Plant. Dis. 105, 1218. https://doi.org/10.1094/PDIS-10-20-2300-PDN (2021).
-
Gonda, I. et al. Genome-based high-resolution mapping of fusarium wilt resistance in sweet basil. Plant. Sci. 321, 111316. https://doi.org/10.1016/j.plantsci.2022.111316 (2022).
-
Karthika, M. & Rasmi, A. R. Pharmacological potential of fungal endophytes associated with the genus Ocimum L. Int. J. Second Metab. 10, 1–10. https://doi.org/10.21448/ijsm.1055749 (2023).
-
Wharton, P. S., Dangi, S. & Woodhall, J. W. Development of an innovative loop-mediated isothermal amplification (LAMP) assay for the rapid on-site detection of Phytophthora infestans. Am. J. Potato Res. 102, 191–204. https://doi.org/10.1007/s12230-025-09986-6 (2025).
-
Thines, M. et al. The genome of Peronospora belbahrii reveals high heterozygosity, a low number of canonical effectors, and TC-rich promoters. Mol. Plant. Microbe Interact. 33, 742–753. https://doi.org/10.1094/MPMI-07-19-0211-R (2020).
-
Bello, J. C., Hausbeck, M. K. & Sakalidis, M. L. Application of target enrichment sequencing for population genetic analyses of the obligate plant pathogens Pseudoperonospora cubensis and P. humuli in Michigan. Mol. Plant. Microbe Interact. 34, 1103–1118. https://doi.org/10.1094/MPMI-11-20-0329-TA (2021).
-
Govers, F. Diseases caused by Oomycetes. In Agrios’ Plant Pathology 435–463 (Academic Press, 2024). https://doi.org/10.1016/B978-0-12-822429-8.00015-7.
-
Stewart, C. N. Jr & Via, L. E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques 14, 748–750 (1993).
-
Standish, J. R. et al. Development, validation, and utility of species-specific diagnostic markers for detection of Peronospora belbahrii. Phytopathology 112, 1667–1675. https://doi.org/10.1094/PHYTO-09-21-0393-R (2022).
