References
-
Rudnicka, E. et al. The World Health Organization (WHO) approach to healthy ageing. Maturitas 139, 6–11 (2020).
-
Izquierdo, M. et al. Global consensus on optimal exercise recommendations for enhancing healthy longevity in older adults (ICFSR). J. Nutr. Health Aging 29, 100401 (2025).
-
Bellavia, D. et al. Vitamin D Level Between Calcium-Phosphorus Homeostasis and Immune System: New Perspective in Osteoporosis. Curr. Osteoporos. Rep. 22, 599–610 (2024).
-
Xu, J., Yu, L., Liu, F., Wan, L. & Deng, Z. The effect of cytokines on osteoblasts and osteoclasts in bone remodeling in osteoporosis: a review. Front. Immunol. 14, 1222129 (2023).
-
Wu, J. et al. Sarcopenia: Molecular regulatory network for loss of muscle mass and function. Front. Nutr. 10, 1037200 (2023).
-
Lee, J.-Y. et al. The animal protein hydrolysate attenuates sarcopenia via the muscle-gut axis in aged mice. Biomed. Pharmacother. 167, 115604 (2023).
-
Meng, S. et al. The prevalence of sarcopenia and risk factors in the older adult in China: a systematic review and meta-analysis. Front. Public Health 12, 1415398 (2024).
-
Defeudis, G., Cardinali, L., Eftekhariranjbar, S., Massari, M. C. & Migliaccio, S. Male osteoporosis: the impact of lifestyle, from nutrition to physical activity. J. Endocrinol. Investig. 48, 1075–1083 (2025).
-
Muniyasamy, R. & Manjubala, I. Insights into the Mechanism of Osteoporosis and the Available Treatment Options. Curr. Pharm. Biotechnol. 25, 1538–1551 (2024).
-
Brito, M. D. et al. Interventions with milk proteins supplementation combined with exercise on musculoskeletal function in older adults with sarcopenia, osteoporosis and osteosarcopenia: a systematic review protocol of randomised controlled trials. BMJ Open 15, e094863 (2025).
-
Marie, B. D. et al. Vitamin D for the prevention of disease: an endocrine society clinical practice guideline. J. Clin. Endocrinol. Metab. 109, 1907–1947 (2024).
-
Toyin, L. et al. The effect of protein intake on bone disease, kidney disease, and sarcopenia: a systematic review. Curr. Dev. Nutr. 9, 104546–104546 (2025).
-
Zakir, S. K. et al. The role of peptides in nutrition: insights into metabolic, musculoskeletal, and behavioral health: a systematic review. Int. J. Mol. Sci. 26, 6043 (2025).
-
Marion, G., Carina, V. & Rosan, M. Clinical presentation and nutrition management of non-IgE-mediated food allergy in children. Clin. Exp. Allergy 55, 213–225 (2025).
-
Hicks, A., Fleischer, D. & Venter, C. The future of cow’s milk allergy—milk ladders in IgE-mediated food allergy. Front. Nutr. 11, 1371772 (2024).
-
Lo et al. The multiple facets of cow’s milk allergy. J. Allergy Clin. Immunol. Pract. 13, 754–760 (2025).
-
Abby, R. et al. Enzymatic hydrolysis altered the physicochemical and immunogenic profile of protein-based ingredients derived from industrial hempseed (Cannabis sativa L.). Food Biosci. 69, 106965–106965 (2025).
-
Kumar Prodhan, U. et al. Circulatory amino acid responses to milk consumption in dairy and lactose intolerant individuals. Eur. J. Clin. Nutr. 76, 1415–1422 (2022).
-
Ziyi, X. et al. Development of hypoallergenic cow’s milk protein peptides via enzymatic hydrolysis coupled with ultrafiltration and analysis of their allergenicity. J. Agric. Food Chem. 73, 30511–30521 (2025).
-
Tang, C., Xi, T., Zheng, J. & Cui, X. Chemical properties of whey protein in protein powders and its impact on muscle growth in athletes: a review. Nat. Prod. Commun. 20 (2025).
-
Czelej, M. et al. Whey protein enzymatic breakdown: synthesis, analysis, and discovery of new biologically active peptides in papain-derived hydrolysates. Molecules 30, 1451 (2025).
-
Zhao, L., Huang, S., Cai, X., Hong, J. & Wang, S. A specific peptide with calcium chelating capacity isolated from whey protein hydrolysate. J. Funct. Foods 10, 46–53 (2014).
-
Chen, H., Yang, L. & Song, T. Amino acid composition analysis and nutritional evaluation of vegetables from Fujian Province. Wei sheng yan jiu = J. Hyg. Res. 49, 978–983 (2020).
-
Toldra, F., Reig, M., Aristoy, M. C. & Mora, L. Generation of bioactive peptides during food processing. Food Chem. 267, 395–404 (2018).
-
Elisa Cruz-Casas, D. et al. Enzymatic hydrolysis and microbial fermentation: the most favorable biotechnological methods for the release of bioactive peptides. Food Chem. Mol. Sci. 3, 100047 (2021).
-
Golkar, A., Milani, J. M. & Vasiljevic, T. Altering allergenicity of cow’s milk by food processing for applications in infant formula. Crit. Rev. Food Sci. Nutr. 59, 159–172 (2019).
-
Ito, K., Okafuji, I., Kawano, M., Ohshima, Y. & Watanabe, Y. Diagnostic usefulness of detecting ige content and profile specific to 14 epitopes defined in cow’s milk allergen. Clin. Exp. Allergy 55, 1130–1133 (2025).
-
Cheison, S. C., Wang, Z. & Xu, S.-Y. Multivariate strategy in screening of enzymes to be used for whey protein hydrolysis in an enzymatic membrane reactor. Int. Dairy J. 17, 393–402 (2007).
-
Ding, M., Duan, M., Wu, S. & Duan, N. Unraveling the mysteries of food allergens: aptamer-driven detection and suppression strategies. Food Res. Int. 219, 117021 (2025).
-
Lao, L. et al. Casein calcium-binding peptides: Preparation, characterization, and promotion of calcium uptake in Caco-2 cell monolayers. Process Biochem. 130, 78–86 (2023).
-
Hu, W. et al. Lactobacillus helveticus-derived whey-calcium chelate promotes calcium absorption and bone health of rats fed a low-calcium diet. Nutrients 16, 1127 (2024).
-
Lei, C. et al. Mechanism analysis of promoting calcium absorption and bone formation of peptides from different casein fractions. Food Biosci. 58 (2024).
-
Zhong, W. et al. Effect of the phosphorylation structure in casein phosphopeptides on the proliferation, differentiation, and mineralization of osteoblasts and its mechanism. Food Funct. 14, 10107–10118 (2023).
-
Fajardo-Espinoza, F. S., Romero-Rojas, A. & Hernandez-Sanchez, H. Production of bioactive peptides from bovine colostrum whey using enzymatic hydrolysis. Rev. Mexicana Ingenieria Quimica 19, 1–9 (2020).
-
Rui, X. U. Calcium binding of peptides derived from enzymatic hydrolysates of whey protein concentrate. Int. J. Dairy Technol. 62, 170–173 (2009).
-
Wang, J. et al. Purification, identification, chelation mechanism, and calcium absorption activity of a novel calcium-binding peptide from peanut (Arachis hypogaea) protein hydrolysate. J. Agric. Food Chem. 71, 11970–11981 (2023).
-
Cai, X., Lin, J. & Wang, S. Novel peptide with specific calcium-binding capacity from Schizochytrium Sp protein hydrolysates and calcium bioavailability in Caco-2 Cells. Mar. Drugs 15, 3 (2017).
-
Liu, G. et al. Promoting the calcium-uptake bioactivity of casein phosphopeptides in vitro and in vivo. Front. Nutr. 8, 743791 (2021).
-
Malison, A., Arpanutud, P. & Keeratipibul, S. Chicken foot broth byproduct: a new source for highly effective peptide-calcium chelate. Food Chem. 345, 128713 (2021).
-
Sun, N. et al. Characterization of sea cucumber (Stichopus japonicus) ovum hydrolysates: calcium chelation, solubility and absorption into intestinal epithelial cells. J. Sci. Food Agric. 97, 4604–4611 (2017).
-
Harizi, N. et al. Amino acids and protein profiles of defatted camel and cow milk fractions: correlation with their in vitro antioxidant and antidiabetic activities. Front. Nutr. 10, 1295878 (2024).
-
Liu, J., Tang, N. & Cheng, Y. Understanding the factors influencing the ability of calcium-binding peptides to promote calcium absorption. ACS Food Sci. Technol. 3, 499–513 (2023).
-
Boye, J., Wijesinha-Bettoni, R. & Burlingame, B. Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method. Br. J. Nutr. 108, S183–S211 (2012).
-
Tomë, D., Bos, C., Mariotti, F. & Gaudichon, C. Protein quality and FAO/WHO recommendations. Sci. Des. Aliments 22, 393–405 (2002).
-
Nations, F.A.O.O., Organization, W.H. & University, U.N. Protein and amino acid requirements in human nutrition : report of a Joint FAO/WHO/UNU Expert Consultation. (Protein and amino acid requirements in human nutrition : report of a Joint FAO/WHO/UNU Expert Consultation, 2007).
-
Adler-Nissen, J. J. E. Enzymic hydrolysis of food proteins (1986).
-
Spellman, D., McEvoy, E., O’Cuinn, G. & FitzGerald, R. J. Proteinase and exopeptidase hydrolysis of whey protein: Comparison of the TNBS, OPA and pH stat methods for quantification of degree of hydrolysis. Int. Dairy J. 13, 447–453 (2003).
-
Fernandez, A. & Kelly, P. pH-stat vs. free-fall pH techniques in the enzymatic hydrolysis of whey proteins. Food Chem. 199, 409–415 (2016).
-
Zhao, L. et al. Measurement of degree of hydrolysis and molecular weight distribution of protein hydrolysates by liquid chromatography-mass spectrometry. Talanta 268, 125347 (2024).
-
Qu, W. et al. Calcium-chelating improved zein peptide stability, cellular uptake, and bioactivity by influencing the structural characterization. Food Res. Int. 162, 112033 (2022).
-
Ding, L. et al. Transport of egg white ACE-inhibitory peptide, Gln-Ile-Gly-Leu-Phe, in human intestinal Caco-2 cell monolayers with cytoprotective effect. J. Agric. Food Chem. 62, 3177–3182 (2014).
-
Chew, L. Y., Toh, G. T., Ismail, A., Shafie, N. H. & Daud, Z. A. M. Casein hydrolysates produced via sequential enzymatic hydrolysis: characterisation and their effect on calcium uptake by Caco-2 cell line. Int. J. Food Sci. Technol. 59, 7952–7961 (2024).
