Quantifying endosomal escape in vivo to guide lipid nanoparticle design

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Quantifying endosomal escape in vivo to guide lipid nanoparticle design
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Nature Biotechnology (2026)Cite this article

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A lysosomal barcoding strategy to quantify endosomal escape of nucleic acids in vivo assesses the performance of branched ionizable lipids for potent liver delivery.

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Fig. 1: Workflow for in vivo quantification of LNPs from endosomal escape.

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Authors and Affiliations

  1. Icahn Genomics Institute, Precision Immunology Institute, Department of Immunology and Immunotherapy, Department of Oncological Sciences, Tisch Cancer Institute, Biomedical Engineering and Imaging Institute, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Zhengwei Liu & Yizhou Dong

Authors

  1. Zhengwei Liu
  2. Yizhou Dong

Corresponding author

Correspondence to Yizhou Dong.

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Competing interests

Y.D. is a co-founder and holds equity in Immunanoengineering Therapeutics.

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Liu, Z., Dong, Y. Quantifying endosomal escape in vivo to guide lipid nanoparticle design. Nat Biotechnol (2026). https://doi.org/10.1038/s41587-026-03047-x

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