Chitosan/pectin bio-composite films enriched with bioactive fraction of Acalypha indica L. as active packaging: a comparative assessment of postharvest fruit quality

Posted on
chitosan/pectin-bio-composite-films-enriched-with-bioactive-fraction-of-acalypha-indica-l.-as-active-packaging:-a-comparative-assessment-of-postharvest-fruit-quality
Chitosan/pectin bio-composite films enriched with bioactive fraction of Acalypha indica L. as active packaging: a comparative assessment of postharvest fruit quality

References

  1. Venkatesan, U. & Muniyan, R. Review on the extension of shelf life for fruits and vegetables using natural preservatives. Food Sci. Biotechnol. 33, 2477–2496 (2024).

    Google Scholar 

  2. Subramani, G. & Manian, R. Bioactive chitosan films: Integrating antibacterial, antioxidant, and antifungal properties in food packaging. Int. J. Biol. Macromol. 278, 134596 (2024).

    Google Scholar 

  3. Ghorbani, M. et al. A halochromic indicator based on polylactic acid and anthocyanins for visual freshness monitoring of minced meat, chicken fillet, shrimp, and fish roe. Innov. Food Sci. Emerg. Technol. 74, 102864 (2021).

    Google Scholar 

  4. Erken, İ, Şahin, S., Karkar, B., Akça, B. & Özakın, C. Chitosan based edible film incorporating different Prunella L. extracts, characterization and their antioxidant properties. J. Food Process. Preserv. 46, e16658 (2022).

    Google Scholar 

  5. Kuswandi, B. & Jumina. 12—Active and intelligent packaging, safety, and quality controls. In Fresh-Cut Fruits and Vegetables (ed. Siddiqui, M. W.) 243–294 (Academic Press, 2020). https://doi.org/10.1016/B978-0-12-816184-5.00012-4.

  6. Medeiros Silva, V. D. et al. Biodegradable edible films of ripe banana peel and starch enriched with extract of Eriobotrya japonica leaves. Food Biosci. 38, 100750 (2020).

    Google Scholar 

  7. Annu, Ali, A. & Ahmed, S. Eco-friendly natural extract loaded antioxidative chitosan/polyvinyl alcohol based active films for food packaging. Heliyon 7, (2021).

  8. Jridi, M. et al. Physicochemical, antioxidant and antibacterial properties of fish gelatin-based edible films enriched with orange peel pectin: Wrapping application. Food Hydrocoll. 103, 105688 (2020).

    Google Scholar 

  9. Barbosa, C. H., Andrade, M. A., Vilarinho, F., Fernando, A. L. & Silva, A. S. Active edible packaging. Encyclopedia 1, 360–370 (2021).

    Google Scholar 

  10. Karkar, B., Patır, İ, Eyüboğlu, S. & Şahin, S. Development of an edible active chitosan film loaded with Nigella sativa L. extract to extend the shelf life of grapes. Biocatal. Agric. Biotechnol. 50, 102708 (2023).

    Google Scholar 

  11. Chegeni, M., Beyranvand, K., Mehri, M. & Dehdashtian, S. Chitosan immobilized by bauxite as an efficient natural catalyst for condensation reaction. J. Inorg. Organomet. Polym. Mater. 34, 735–744 (2024).

    Google Scholar 

  12. Atarés, L. & Chiralt, A. Essential oils as additives in biodegradable films and coatings for active food packaging. Trends Food Sci. Technol. 48, 51–62 (2016).

    Google Scholar 

  13. Lei, Y. et al. Investigation of the structural and physical properties, antioxidant and antimicrobial activity of pectin-konjac glucomannan composite edible films incorporated with tea polyphenol. Food Hydrocoll. 94, 128–135 (2019).

    Google Scholar 

  14. Shivangi, S., Dorairaj, D., Negi, P. S. & Shetty, N. P. Development and characterisation of a pectin-based edible film that contains mulberry leaf extract and its bio-active components. Food Hydrocoll. 121, 107046 (2021).

    Google Scholar 

  15. Zahidin, N. S. et al. A review of Acalypha indica L. (Euphorbiaceae) as traditional medicinal plant and its therapeutic potential. J. Ethnopharmacol. 207, 146–173 (2017).

    Google Scholar 

  16. Refilda, Oktafia, N., Winardi, P. R., Salim, E., & Yefrida. Utilization of Aloe vera gel and Acalypha indica L. leaf extract as edible coating to increase the shelf life of guava (Psidium guajava L.) fruit. IOP Conf. Ser. Earth Environ. Sci. 1059, 012048 (2022).

  17. Menon, S., Agarwal, H., Kumar, S. R. & Kumar, S. V. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Int. J. Appl. Pharm. 9, 42 (2017).

    Google Scholar 

  18. Venkatesan, U. & Muniyan, R. Therapeutic potential of Acalypha indica L. leaf fractions against foodborne pathogens: An in vitro and in silico study. Sci. Rep. 16, 2423 (2025).

    Google Scholar 

  19. Gini, T. G. & Jeya Jothi, G. Column chromatography and HPLC analysis of phenolic compounds in the fractions of Salvinia molesta Mitchell. Egypt. J. Basic Appl. Sci. 5, 197–203 (2018).

    Google Scholar 

  20. Rajkumar, V., Guha, G. & Kumar, R. A. Isolation and bioactivity evaluation of two metabolites from the methanolic extract of Oroxylum indicum stem bark. Asian Pac. J. Trop. Biomed. 2, S7–S11 (2012).

    Google Scholar 

  21. Nguyen, T. T. T. et al. Characterizations and antibacterial activities of passion fruit peel pectin/chitosan composite films incorporated Piper betle L. leaf extract for preservation of purple eggplants. Heliyon https://doi.org/10.1016/j.heliyon.2022.e10096 (2022).

    Google Scholar 

  22. Sun, L. et al. Preparation and characterization of chitosan film incorporated with thinned young apple polyphenols as an active packaging material. Carbohydr. Polym. 163, 81–91 (2017).

    Google Scholar 

  23. Adımcılar, V., Torlak, E. & Erim, F. B. Intelligent pectin and alginate-based biopolymeric film enriched with purple basil anthocyanins for pH-sensitive detection of chicken meat freshness. ACS Omega https://doi.org/10.1021/acsomega.5c01438 (2025).

    Google Scholar 

  24. Wang, X. et al. Development and characterization of zein/gum Arabic nanocomposites incorporated edible films for improving strawberry preservation. Adv. Compos. Hybrid Mater. 7, 249 (2024).

    Google Scholar 

  25. El Habbasha, E. S. et al. Developing a novel, low-cost, antimicrobial, and biodegradable pectin/HEC/ZnO biofilm for edible food packaging applications. Biomass Convers. Biorefinery 15, 6377–6388 (2025).

    Google Scholar 

  26. Hartwig, V. G., Brumovsky, L. A., Fretes, R. M. & Boado, L. S. A novel procedure to measure the antioxidant capacity of Yerba maté extracts. Food Sci. Technol. 32, 126–133 (2012).

    Google Scholar 

  27. Jatav, J., Chinchkar, A. V. & Bhattacharya, B. Chitosan film with pineapple peel extract in the extension of shelf life of Indian cottage cheese: Release kinetics and bio-accessibility studies. Food Res. Int. 174, 113580 (2023).

    Google Scholar 

  28. Bharathi, S. K. V., Leena, M. M., Moses, J. A. & Anandharamakrishnan, C. Nanofibre-based bilayer biopolymer films: Enhancement of antioxidant activity and potential for food packaging application. Int. J. Food Sci. Technol. 55, 1477–1484 (2020).

    Google Scholar 

  29. Tomadoni, B., Cassani, L., Ponce, A., Moreira, M. R. & Agüero, M. V. Optimization of ultrasound, vanillin and pomegranate extract treatment for shelf-stable unpasteurized strawberry juice. LWT – Food Sci. Technol. 72, 475–484 (2016).

    Google Scholar 

  30. Jancikova, S., Dordevic, D., Tesikova, K., Antonic, B. & Tremlova, B. Active edible films fortified with natural extracts: Case study with fresh-cut apple pieces. Membranes 11, 684 (2021).

    Google Scholar 

  31. Özkara, A., Akyıl, D., Eren, Y. & Erdoğmuş, S. F. Potential cytotoxic effect of Anilofos by using Allium cepa assay. Cytotechnology 67, 783–791 (2015).

    Google Scholar 

  32. Alabi, O. A., Atanda, H. C. & Olumurewa, J. A. V. Cytogenotoxicity of the aqueous extract of Parquetina nigrescens leaf using Allium cepa assay. Protoplasma 259, 1417–1425 (2022).

    Google Scholar 

  33. Ihegboro, G. O., Alhassan, A. J., Ononamadu, C. J., Owolarafe, T. A. & Sule, M. S. Evaluation of the biosafety potentials of methanol extracts/fractions of Tapinanthus bangwensis and Moringa oleifera leaves using Allium cepa model. Toxicol. Rep. 7, 671–679 (2020).

    Google Scholar 

  34. S., M. E., El-Sherbiny, G. M., Sharaf, M. H., Kalaba, M. H. & Shaban, A. S. Phytochemical analysis, antimicrobial, antioxidant, and cytotoxicity activities of Schinus molle (L.) extracts. Biomass Convers. Biorefinery 15, 3753–3770 (2025).

    Google Scholar 

  35. Kaya, M. et al. Antioxidative and antimicrobial edible chitosan films blended with stem, leaf and seed extracts of Pistacia terebinthus for active food packaging. RSC Adv. 8, 3941–3950 (2018).

    Google Scholar 

  36. Ribeiro, A. M., Estevinho, B. N. & Rocha, F. Preparation and incorporation of functional ingredients in edible films and coatings. Food Bioprocess Technol. 14, 209–231 (2021).

    Google Scholar 

  37. Martins, J. T., Cerqueira, M. A. & Vicente, A. A. Influence of α-tocopherol on physicochemical properties of chitosan-based films. Food Hydrocoll. 27, 220–227 (2012).

    Google Scholar 

  38. Nisar, T. et al. Functionality of different probiotic strains embedded in citrus pectin based edible films. Int. J. Food Sci. Technol. 57, 1005–1015 (2022).

    Google Scholar 

  39. Chen, J. et al. Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids. Sci. Rep. 10, 2611 (2020).

    Google Scholar 

  40. Ekrami, M., Emam-Djomeh, Z., Ghoreishy, S. A., Najari, Z. & Shakoury, N. Characterization of a high-performance edible film based on Salep mucilage functionalized with pennyroyal (Mentha pulegium). Int. J. Biol. Macromol. 133, 529–537 (2019).

    Google Scholar 

  41. Zhang, F. et al. Antimicrobial, anti-biofilm properties of three naturally occurring antimicrobial peptides against spoilage bacteria, and their synergistic effect with chemical preservatives in food storage. Food Control 123, 107729 (2021).

    Google Scholar 

  42. Cui, L. et al. Preparation and characterization of chitosan membranes. RSC Adv. 8, 28433–28439 (2018).

    Google Scholar 

  43. Qin, C., Yang, G., Zhu, C. & Wei, M. Characterization of edible film fabricated with HG-type hawthorn pectin gained using different extraction methods. Carbohydr. Polym. 285, 119270 (2022).

    Google Scholar 

  44. Sembiring, E., Frida, E., Sitorus, Z. & Sembiring, T. Fabrication and characterization of pectin-chitosan edible coatings with a Cosmos caudatus leaf extract for tomato preservation. ACS Omega 10, 7204–7210 (2025).

    Google Scholar 

  45. Mobasserfar, R., Shiri, A., Mofid, V., Noghabi, M. S. & Gharibzahedi, S. M. T. Optimizing, characterizing, and unveiling of functional properties and structure of grape pomace pectin obtained by a sequential ultrasound-microwave extraction. Biomass Convers. Biorefinery 15, 8621–8634 (2025).

    Google Scholar 

  46. Valáriková, J., Čížová, A., Račková, L. & Bystrický, S. Anti-staphylococcal activity of quaternized mannan from the yeast Candida albicans. Carbohydr. Polym. 240, 116288 (2020).

    Google Scholar 

  47. Guo, Q. et al. Non-starch polysaccharides from American ginseng: Physicochemical investigation and structural characterization. Food Hydrocoll. 44, 320–327 (2015).

    Google Scholar 

  48. Jaiwal, B. V., Shaikh, F. K., Bradosty, S. W., Patil, A. B. & Hallur, R. L. S. Identification of matrix metalloproteinases inhibitors from methanol extract of Peltophorum Pterocarpum leaves. J. Appl. Biol. Biotechnol. 11, 92–98 (2023).

    Google Scholar 

  49. Mohammadian, M., Moghaddam, A. D., Sharifan, A., Dabaghi, P. & Hadi, S. Structural, physico-mechanical, and bio-functional properties of whey protein isolate-based edible films as affected by enriching with nettle (Urtica dioica L.) leaf extract. J. Food Meas. Charact. 15, 4051–4060 (2021).

    Google Scholar 

  50. Kumar, R., Ghoshal, G. & Goyal, M. Effect of basil leaves extract on modified moth bean starch active film for eggplant surface coating. LWT 145, 111380 (2021).

    Google Scholar 

  51. Priyadarshi, R. et al. Chitosan film incorporated with citric acid and glycerol as an active packaging material for extension of green chilli shelf life. Carbohydr. Polym. 195, 329–338 (2018).

    Google Scholar 

  52. Ekrami, M., Emam-Djomeh, Z., Ghoreishy, S. A., Najari, Z. & Shakoury, N. Characterization of a high-performance edible film based on Salep mucilage functionalized with pennyroyal (*Mentha pulegium*). Int. J. Biol. Macromol. 133, 529–537 (2019).

    Google Scholar 

  53. Enayat, S. & Banerjee, S. Comparative antioxidant activity of extracts from leaves, bark and catkins of *Salix aegyptiaca* sp. Food Chem. 116, 23–28 (2009).

    Google Scholar 

  54. Wang, W. et al. Characterization of pectin from grapefruit peel: A comparison of ultrasound-assisted and conventional heating extractions. Food Hydrocoll. 61, 730–739 (2016).

    Google Scholar 

  55. Pu, Y. et al. Techno-functional properties of active film based on guar gum-propolis and its application for “Nanguo” pears preservation. Int. J. Biol. Macromol. 261, 129578 (2024).

    Google Scholar 

  56. Yong, H. & Liu, J. Active packaging films and edible coatings based on polyphenol-rich propolis extract: A review. Compr. Rev. Food Sci. Food Saf. 20, 2106–2145 (2021).

    Google Scholar 

  57. Vimala Bharathi, S. K., Maria Leena, M., Moses, J. A. & Anandharamakrishnan, C. Zein-based anti-browning cling wraps for fresh-cut apple slices. Int. J. Food Sci. Technol. 55, 1238–1245 (2020).

    Google Scholar 

  58. El Mouzahim, M. et al. Food packaging composite film based on chitosan, natural kaolinite clay, and Ficus. carica leaves extract for fresh-cut apple slices preservation. Int. J. Biol. Macromol. 233, 123430 (2023).

    Google Scholar 

Download references

Related Posts