Preharvest application of plant growth regulators extends harvest window and improves yield and quality of mandarin orange cv. Khoku Local

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Preharvest application of plant growth regulators extends harvest window and improves yield and quality of mandarin orange cv. Khoku Local

References

  1. Mansoor, S. & Kim, I. J. Classical to modern biotechnology approaches, applications and future prospects in citrus breeding. Plant. Biotechnol. Rep. 2024, 1–15. https://doi.org/10.1007/s11816-024-00949-7 (2024).

  2. Lado, J., Cuellar, F., Rodrigo, M. J. & Zacarías, L. Nutritional composition of mandarins. In Nutritional Composition of Fruit Cultivars 419–443 (Academic Press, 2016). https://doi.org/10.1016/B978-0-12-408117-8.00018-0.

  3. Gill, K. et al. Physiological perspective of plant growth regulators in flowering, fruit setting and ripening process in citrus. Sci. Hort. 309, 111628. https://doi.org/10.1016/j.scienta.2022.111628 (2023).

    Google Scholar 

  4. Bons, H. K. & Kaur, M. Role of plant growth regulators in improving fruit set, quality and yield of fruit crops: a review. J. Hortic. Sci. Biotechnol. 95 (2), 137–146. https://doi.org/10.1080/14620316.2019.1660591 (2020).

    Google Scholar 

  5. Bermejo, A. et al. Auxin and gibberellin interact in citrus fruit set. J. Plant Growth Regul. 37 (2), 491–501. https://doi.org/10.1007/s00344-017-9748-9 (2018).

    Google Scholar 

  6. Choudhary, H. D., Jain, M. C., Sharma, M. K. & Bhatnagar, P. Effect of plant growth regulators on growth and yield of Nagpur mandarin (Citrus reticulata Blanco.). Food Chem.: X 8(2), 746–750 (2013).

  7. Nawaz, M. A. et al. Exogenous application of 2, 4-D, GA3 and NAA at flowering improves yield and quality of Kinnow Mandarin (Citrus reticulata Blanco). Asian Australasian J. Plant. Sci. Biotechnol. 5 (1), 17–21 (2011).

    Google Scholar 

  8. Aziz, L. et al. Practical implications of PGRs in improving fruit juice quality of Citrus reticulate. Russ. J. Plant Physiol. 72 (4), 110. https://doi.org/10.1134/S1021443725601557 (2025).

    Google Scholar 

  9. Bons, H. K., Kaur, N. & Rattanpal, H. S. Quality and quantity improvement of citrus: role of plant growth regulators. Int. J. Agric. Environ. Biotechnol. 8 (2), 433–447 (2015).

    Google Scholar 

  10. El-Otmani, M., Coggins, C. W. Jr, Agusti, M. & Lovatt, C. J. Plant growth regulators in citriculture: world current uses. CRC. Crit. Rev. Plant Sci. 19 (5), 395–447. https://doi.org/10.1080/07352689.2000.10131824 (2000).

    Google Scholar 

  11. Lin, H. et al. Effects of auxin applications on grape berries delays maturation by inhibiting cell wall degradation. Sci. Hort. 343, 114085. https://doi.org/10.1016/j.scienta.2025.114085 (2025).

    Google Scholar 

  12. Trejo, E. O. et al. The impact of PGRs applied in the field on the postharvest behavior of fruit crops. Sci. Hort. 318, 112103. https://doi.org/10.1016/j.scienta.2023.112103 (2023).

    Google Scholar 

  13. Nawaz, M. A., Ahmad, W., Ahmad, S. & Khan, M. M. Role of growth regulators on preharvest fruit drop, yield and quality in Kinnow mandarin. Pak J. Bot. 40 (5), 1971–1981 (2008).

    Google Scholar 

  14. Almeida, I. M. L. D., Rodrigues, J. D. & Ono, E. O. Application of plant growth regulators at pre-harvest for fruit development of’PÊRA’oranges. Braz. Arch. Biol. Technol. 47, 511–520 (2004).

    Google Scholar 

  15. Khokhar, Y. Exploring ecological safety and a potential approach for regulating the preharvest ‘Kinnow’Fruit drop under lower Himalayas. Appl. Fruit Sci. 66 (6), 2329–2336. https://doi.org/10.1007/s10341-024-01221-6 (2024).

    Google Scholar 

  16. Singh, S. et al. Management of pre-harvest fruit drop and ultrastructural studies of ‘Kinnow’Mandarin under subtropical conditions. Appl. Fruit Sci. 67 (2), 1–9. https://doi.org/10.1007/s10341-025-01286-x (2025).

    Google Scholar 

  17. Starkus, A. et al. The biological and genetic mechanisms of fruit drop in apple tree (Malus× domestica Borkh). Horticulturae 10 (9), 987. https://doi.org/10.3390/horticulturae10090987 (2024).

    Google Scholar 

  18. Cai, N., Chen, C., Wan, C. & Chen, J. Effects of pre-harvest gibberellic acid spray on endogenous hormones and fruit quality of kumquat (Citrus japonica) fruits. New. Z. J. Crop Hortic. Sci. 49 (2–3), 211–224. https://doi.org/10.1080/01140671.2020.1806084 (2021).

    Google Scholar 

  19. Khan, E. U. & Ali, L. Effect of foliar application of gibberellic acid (GA3) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) on yield and fruit quality of low seeded kinnow mandarin (Citrus reticulata Blanco). In Proceedings of Pakistan Society for Horticultural Science, 2nd International Conference on Horticultural Sciences; Punjab, Pakistan 18–20 (2016).

  20. Gene Albrigo, L. & Galán Saúco, V. Flower bud induction, flowering and fruit-set of some tropical and subtropical fruit tree crops with special reference to citrus. Acta Hortic. 632, 81–90. https://doi.org/10.17660/ActaHortic.2004.632.10 (2004).

    Google Scholar 

  21. Fahad, S. & Rab, A. Association of gibberellic acid (GA3) with fruit set and fruit drop of sweet orange. J. Biol. Agric. Healthc. 4 (2), 54–59 (2014).

    Google Scholar 

  22. Gambetta, G. et al. Gibberellic acid and norflurazon affecting the time-course of flavedo pigment and abscisic acid content in ‘Valencia’sweet orange. Sci. Hort. 180, 94–101. https://doi.org/10.1016/j.scienta.2014.10.021 (2014).

    Google Scholar 

  23. Garmendia, A. et al. Gibberellic acid in Citrus spp. flowering and fruiting: a systematic review. PloS one. 14 (9), e0223149. https://doi.org/10.1371/journal.pone.0223147 (2019).

    Google Scholar 

  24. Hiteshbhai, R., Johar, V. & Singh, V. Effect of plant growth regulators on fruit set and quality of kinnow mandarin (Citrus reticulata Blanco). Environ. Ecol. 41, 7–12 (2023). ISSN: 0970 – 0420.

    Google Scholar 

  25. Adhikary, T., Chattopadhyay, P. & Gill, S. P. Plant growth regulators in citrus. In Plant Growth Regulators in Tropical and Sub-tropical Fruit Crops 113–142 (CRC, 2022).

  26. Saleem, B. A., Malik, A. U., Pervez, M. A. & Khan, A. S. Growth regulators application affects the vegetative and reproductive behaviour of ‘Blood Red’sweet orange. Pak J. Bot. 40 (5), 2115–2125 (2008).

    Google Scholar 

  27. Nartvaranant, P. The influence of exogenously applied 2, 4-D and NAA on fruit drop reduction in pummelo cv. Thong Dee. Int. J. Fruit Sci. 18 (2), 215–235. https://doi.org/10.1080/15538362.2017.1422449 (2018).

    Google Scholar 

  28. Ashraf, M. Y., Ashraf, M., Akhtar, M., Mahmood, K. & Saleem, M. U. H. A. M. M. A. D. Improvement in yield, quality and reduction in fruit drop in kinnow (Citrus reticulata Blanco) by exogenous application of plant growth regulators, potassium and zinc. Pak. J. Bot. 45 (S1), 433–440 (2013).

    Google Scholar 

  29. Stover, E. W. & Greene, D. W. Environmental effects on the performance of foliar applied plant growth regulators: A review focusing on tree fruits. HortTechnology 15 (2), 214 (2005).

    Google Scholar 

  30. DHM. Meteorological Data. Department of Hydrology and Meteorology, Government of Nepal (2020).

  31. Davies, F. S. & Albrigo, L. G. Fruit quality, harvesting and postharvest technology. In Citrus London: CAB International: HK 202–224 (1994).

  32. Lado, J., Rodrigo, M. J. & Zacarías, L. Maturity indicators and citrus fruit quality. Stewart Postharvest Rev. 10 (2), 1–6 (2014).

    Google Scholar 

  33. AOAC. Official Methods of Analysis (Association of Official Analytical Chemists, 2005).

  34. Sadasivam, S. & Balasubramanian, T. Practical manual in biochemistry. In Tamil Nadu Agricultural University, Coimbatore, India 14 (1987).

  35. Devi, L. S. et al. Natural wax-based edible coatings for preserving postharvest quality of mandarin orange. Food Chem.: X. 26, 102302 (2025).

    Google Scholar 

  36. Erogul, D. & Sen, F. The effect of preharvest gibberellic acid applications on fruit quality of ʽAngelinoʼ plums during storage. Sci. Hort. 202, 111–116. https://doi.org/10.1016/j.scienta.2016.02.027 (2016).

    Google Scholar 

  37. Li, H. et al. Gibberellins play a role in regulating tomato fruit ripening. Plant Cell Physiol. 60 (7), 1619–1630. https://doi.org/10.1093/pcp/pcz069 (2019).

    Google Scholar 

  38. Ozkan, Y., Ucar, M., Yildiz, K. & Ozturk, B. Pre-harvest gibberellic acid (GA3) treatments play an important role on bioactive compounds and fruit quality of sweet cherry cultivars. Sci. Hort. 211, 358–362. https://doi.org/10.1016/j.scienta.2016.09.019 (2016).

    Google Scholar 

  39. Wu, M. et al. Gibberellins are involved in fruit ripening and softening by mediating multiple hormonal signals in tomato. Hortic. Res. 11 (2), uhad275. https://doi.org/10.1093/hr/uhad275 (2024).

    Google Scholar 

  40. Li, Q. et al. PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance. J. Exp. Bot. 66 (13), 3765–3774. https://doi.org/10.1093/jxb/erv169 (2015).

    Google Scholar 

  41. Liao, X. et al. Interlinked regulatory loops of ABA catabolism and biosynthesis coordinate fruit growth and ripening in woodland strawberry. Proc. Natl. Acad. Sci. 115 (49), E11542–E11550. https://doi.org/10.1073/pnas.1812575115 (2018).

    Google Scholar 

  42. Park, M. H. & Malka, S. K. Gibberellin delays metabolic shift during tomato ripening by inducing auxin signaling. Front. Plant Sci. 13, 1045761. https://doi.org/10.3389/fpls.2022.1045761 (2022).

    Google Scholar 

  43. Shi, Y. et al. Molecular regulatory events of flower and fruit abscission in horticultural plants. Hortic. Plant. J. 9 (5), 867–883. https://doi.org/10.1016/j.hpj.2023.03.008 (2023).

    Google Scholar 

  44. Zhao, M. & Li, J. Molecular events involved in fruitlet abscission in litchi. Plants 9 (2), 151. https://doi.org/10.3390/plants9020151 (2020).

    Google Scholar 

  45. Adouli, B., Zamani, Z., Fattahi-Mohgadam, M. R., Golein, B. & Rezaei, K. Effects of alternate bearing and 2, 4-D application on fruit growth pattern, abscission enzymes activity, ACC content of calyx and carbohydrates partitioning of fruits in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa. Sci. Hort. 238, 58–65. https://doi.org/10.1016/j.scienta.2018.04.042 (2018).

    Google Scholar 

  46. Gurjar, P. S. & Rana, G. S. Influence of foliar application of nutrients and growth regulator on fruit drop, yield and fruit size and quality in Kinnow mandarin. Indian J. Hortic. 71 (1), 109–111 (2014).

    Google Scholar 

  47. Ren, B. et al. Response of abscission zone of blue honeysuckle (Lonicera caerulea L.) fruit to GA3 and 2, 4-D spray application. Agronomy 13 (12), 2937. https://doi.org/10.3390/agronomy13122937 (2023).

    Google Scholar 

  48. Wang, H. et al. PbGA20ox2 regulates fruit set and induces parthenocarpy by enhancing GA4 content. Front. Plant Sci. 11, 113. https://doi.org/10.3389/fpls.2020.00113 (2020).

    Google Scholar 

  49. Iqbal, N., Nazar, R., Khan, M. I. R., Masood, A. & Khan, N. A. Role of gibberellins in regulation of source-sink relations under optimal and limiting environmental conditions. Curr. Sci. 2011, 998–1007. http://www.jstor.org/stable/24076517 (2011).

  50. Li, W. F. et al. Regulatory mechanism of GA3 application on grape (Vitis vinifera L.) berry size. Plant Physiol. Biochem. 210, 108543. https://doi.org/10.1016/j.plaphy.2024.108543 (2024).

    Google Scholar 

  51. Talat, H. et al. Effect of gibberellic acid on fruit quality of Kinnow mandarin. J. Glob Innov. Agric. Soc. Sci. 8, 59–63. https://doi.org/10.22194/JGIASS/8.901 (2020).

    Google Scholar 

  52. Lal, D. et al. Pre-harvest spray of gibberellic acid, NAA, and calcium nitrate on fruit retention, yield and quality of kinnow mandarin. Environ. Ecol. 34 (4 C), 2288–2292 (2016).

    Google Scholar 

  53. Dong, Y., Zhi, H. & Wang, Y. Cooperative effects of pre-harvest calcium and gibberellic acid on tissue calcium content, quality attributes, and in relation to postharvest disorders of late-maturing sweet cherry. Sci. Hort. 246, 123–128. https://doi.org/10.1016/j.scienta.2018.10.067 (2019).

    Google Scholar 

  54. Rokaya, P. R., Baral, D. R., Gautam, D. M., Shrestha, A. K. & Paudyal, K. P. Effect of pre-harvest application of gibberellic acid on fruit quality and shelf life of mandarin (Citrus reticulata Blanco). Am. J. Plant. Sci. 7 (07), 1033. https://doi.org/10.4236/ajps.2016.77098 (2016).

    Google Scholar 

  55. El-Shereif, A. R., Zaghloul, A. E. S. & Abu Elyazid, D. M. Effect of streptomycin and GA3 application on seedlessness, yield and fruit quality of ’Balady’ Mandarin. Egypt. J. Hortic. 44 (1), 99–104. https://doi.org/10.21608/ejoh.2017.1178.1012 (2017).

    Google Scholar 

  56. Ma, Q. et al. Comprehensive insights on how 2, 4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches. J. Exp. Bot. 65 (1), 61–74. https://doi.org/10.1093/jxb/ert344 (2014).

    Google Scholar 

  57. Chen, S., Wang, X. J., Tan, G. F., Zhou, W. Q. & Wang, G. L. Gibberellin and the plant growth retardant Paclobutrazol altered fruit shape and ripening in tomato. Protoplasma 257 (3), 853–861. https://doi.org/10.1007/s00709-019-01471-2 (2020).

    Google Scholar 

  58. Feng, T. et al. Gibberellins and their interplay with other hormones in the regulation of fruit ripening. Hortic. Plant. J. https://doi.org/10.1016/j.hpj.2025.04.006 (2025).

    Google Scholar 

  59. Li, M. et al. VvARF19 represses VvLBD13-mediated cell wall degradation to delay softening of grape berries. Hortic. Res. 12 (2), uhae322. https://doi.org/10.1093/hr/uhae322 (2025).

    Google Scholar 

  60. Knoche, M. & Peschel, S. Gibberellins increase cuticle deposition in developing tomato fruit. Plant. Growth Regul. 51 (1), 1–10. https://doi.org/10.1007/s10725-006-9107-5 (2007).

    Google Scholar 

  61. Li, Z. X. et al. Widely targeted metabolomics analysis reveals the effect of exogenous auxin on postharvest resistance to Botrytis cinerea in kiwifruit (Actinidia chinensis L.). Postharvest Biol. Technol. 195, 112129. https://doi.org/10.1016/j.postharvbio.2022.112129 (2023).

    Google Scholar 

  62. Manzoor, M. et al. Effects of harvest time on the fruit quality of Kinnow and Feutrell’s early mandarins (Citrus reticulata Blanco). Agronomy 13 (3), 802. https://doi.org/10.3390/agronomy13030802 (2023).

    Google Scholar 

  63. Rezk, A. et al. Preharvest Mandarin rind disorder: insights into varietal differences and preharvest treatments effects on postharvest quality. Plants 13 (8), 1040. https://doi.org/10.3390/plants13081040 (2024).

    Google Scholar 

  64. Khan, A. S. & Ali, S. Preharvest sprays affecting shelf life and storage potential of fruits. In Preharvest Modulation of Postharvest Fruit and Vegetable Quality 209–255 (Academic Press, 2018). https://doi.org/10.1016/B978-0-12-809807-3.00009-3.

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