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Light-induced extracellular vesicle and particle adsorption (LEVA) is a micropatterning technique that uses precisely controlled light exposure to position extracellular vesicles and other particles (EVPs) on surfaces with high resolution. By creating complex microscale patterns and gradients of EVPs, LEVA enables the study of how cells interact with surface-bound EVPs and single-EVP analyses.
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References
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Kolesov, A. et al. Migrasomes, matrix-bound nanovesicles, and more: messengers in the matrix. Preprint at Authorea https://doi.org/10.22541/au.174315962.29311717/v1 (2025). A perspective article that summarizes what is currently known about matrix-bound EVPs.
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Strale, P. O. et al. Multiprotein printing by light-induced molecular adsorption. Adv. Mater. 28, 2024–2029 (2016). A paper that reports the development of LIMAP for micropatterning proteins by light-induced adsorption.
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Welsh, J. et al. Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J. Extracell. Vesicles 13, e12404 (2024). A position paper that provides researchers with a snapshot of best practices for EV production, isolation, characterization, and nomenclature.
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Ma, L. et al. Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res. 25, 24–38 (2015). A paper that reports the discovery of migrasomes and migracytosis.
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Zhang, J. et al. Engineering a tunable micropattern-array assay to sort single extracellular vesicles and particles to detect RNA and protein in situ. J. Extracell. Vesicles 12, e12369 (2023). A paper that demonstrates UV micropatterning of EVPs combined with immunoaffinity surface immobilization.
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This is a summary of: Hisey, C. L. et al. Light-induced extracellular vesicle and particle adsorption. Nat. Methods https://doi.org/10.1038/s41592-025-02914-w (2025).
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A light-induced micropatterning technique for extracellular vesicles and particles. Nat Methods (2025). https://doi.org/10.1038/s41592-025-02915-9
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DOI: https://doi.org/10.1038/s41592-025-02915-9
