A live, programmable microbiota therapeutic disables virulence in gut pathogens

a-live,-programmable-microbiota-therapeutic-disables-virulence-in-gut-pathogens
A live, programmable microbiota therapeutic disables virulence in gut pathogens
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Nature Biomedical Engineering (2025)Cite this article

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Treatment of severe gastrointestinal illness caused by Shiga-toxin-producing enteropathogenic bacteria is a growing challenge owing to increasing antimicrobial resistance. We engineered a conjugative CRISPR-associated transposase to insert a genetic payload that inactivates the Shiga toxin gene of Enterobacteriaceae pathogens in the gut, establishing a foundation for microbial gene therapy.

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Fig. 1: In situ pathogen inactivation in the gut using BACTRINS.

References

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This is a summary of: Ronda, C. et al. Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-025-01453-1 (2025).

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A live, programmable microbiota therapeutic disables virulence in gut pathogens. Nat. Biomed. Eng (2025). https://doi.org/10.1038/s41551-025-01452-2

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  • DOI: https://doi.org/10.1038/s41551-025-01452-2