A lysine-free REC tag system for proximity-biotinylation applications

a-lysine-free-rec-tag-system-for-proximity-biotinylation-applications
A lysine-free REC tag system for proximity-biotinylation applications

Data availability

All data generated or analysed during this study are included in this published article, its supplementary information, and source data files. Information and data in this article are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to thank the Applied Protein Research Laboratory at Ehime University. This work was supported by a grant from the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research [BINDS]) from AMED under Grant Numbers JP24ama121010 and JP25ama121010 (T.S. and T.O.); AMED grant numbers JP23fk0310521 (T.S.); KAKENHI (21K19230 and 24H00560 to T.S.) from the Japan Society for the Promotion of Science (JSPS); a Grant-in-Aid for JSPS Fellows (24KJ1755 for K.Y.) from JSPS; and Joint Usage and Joint Research Programs of the Institute of Advanced Medical Sciences of Tokushima University. We would like to thank Editage (www.editage.com) for the English language editing.

Author information

Author notes

  1. Soh Tokunaga

    Present address: Forensic Science Laboratory, Ehime Prefectural Police Headquarters, 2-2 Minamihoribatacho, Matsuyama, Ehime, 790-8573, Japan

Authors and Affiliations

  1. Division of Cell-Free Sciences, Proteo-Science Center, PIAS, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan

    Soh Tokunaga, Kohdai Yamada, Hinako Fujisaki, Mana Hamada, Fumiya Soga & Tatsuya Sawasaki

  2. Division of Malaria Research, Proteo-Science Center, PIAS, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan

    Hikaru Nagaoka & Eizo Takashima

  3. Department of Life Sciences and Bioengineering, Laboratory of Molecular and Cellular Biology, Faculty of Engineering, Academic Assembly, University of Toyama, Gofuku, Toyama-shi, Toyama, 3190, 930-8555, Japan

    Tatsuhiko Ozawa

  4. Center for Advanced Antibody Drug Development, University of Toyama, Gofuku, Toyama-shi, Toyama, 3190, 930-8555, Japan

    Tatsuhiko Ozawa & Hiroyuki Kishi

  5. Division of Proteo-Drug-Discovery Sciences, Proteo-Science Center, PIAS, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan

    Hiroyuki Takeda

  6. Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, 930-0194, Japan

    Hiroyuki Kishi

  7. Proteo-Science Center, PIAS, Ehime University, Matsuyama, 790-8577, Japan

    Tatsuya Sawasaki

Authors

  1. Soh Tokunaga
  2. Hikaru Nagaoka
  3. Tatsuhiko Ozawa
  4. Kohdai Yamada
  5. Hiroyuki Takeda
  6. Hinako Fujisaki
  7. Mana Hamada
  8. Fumiya Soga
  9. Hiroyuki Kishi
  10. Eizo Takashima
  11. Tatsuya Sawasaki

Contributions

S.T. performed the cloning of antibody genes, antibody characterisation, antibody synthesis, epitope mapping, cell-based assays, and biotinylation assay; K.Y., F.S., H.F., and M.H. supported the cloning of antibody genes, antibody synthesis, antibody characterisation, and cell-based assays; H.T. performed SPR analysis; H.N. and E.T. performed immunoblotting of the malarial parasite and synthesis of PfRipr5 antigen protein; T.O. and H.K. performed ISAAC; T.S. conceived the research, designed the experiments, and analysed the data; S.T. and T.S. wrote the manuscript; all authors revised the manuscript.

Corresponding author

Correspondence to Tatsuya Sawasaki.

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The authors declare no competing interests.

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Tokunaga, S., Nagaoka, H., Ozawa, T. et al. A lysine-free REC tag system for proximity-biotinylation applications. Sci Rep (2026). https://doi.org/10.1038/s41598-026-35323-w

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  • DOI: https://doi.org/10.1038/s41598-026-35323-w