A next-generation dual guide CRISPR system for genetic interaction library screening

The code used to generate analysis has been deposited in the Zenodo database (https://doi.org/10.5281/zenodo.17191951)^44,45 under a Creative Commons Attribution 4.0 International license (CC BY 4.0). Additional information for the following analyses can be found on the links below. Pilot library design: https://github.com/EmanuelGoncalves/crispy/blob/master/notebooks/dualguide/Library2Composition.py Genetic interaction library design: https://github.com/ibarrioh/DualGuide_COLO1/ Screen analysis: https://github.com/ibarrioh/DualGuide_COLO1/.

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Authors and Affiliations

1. Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK

   Thomas Burgold, Emre Karakoc, Emanuel Gonçalves, Inigo Barrio-Hernandez, Lisa Dwane, Romina Silva, Emily Souster, Mamta Sharma, Alexandra Beck, Gene Ching Chiek Koh, Mathew J. Garnett & Andrew R. Bassett

2. OpenTargets, Wellcome Genome Campus, Hinxton, Cambridge, UK

   Emre Karakoc, Inigo Barrio-Hernandez, Romina Silva, Emily Souster, Alexandra Beck, Lykourgos-Panagiotis Zalmas, Mathew J. Garnett & Andrew R. Bassett

3. Instituto Superior Técnico (IST), Universidade de Lisboa, Lisboa, Portugal

   Emanuel Gonçalves

4. INESC-ID, Lisboa, Portugal

   Emanuel Gonçalves

5. Instituto de Agrobiotecnología, IdAB, CSIC-Gobierno de Navarra, Mutilva, Spain

   Inigo Barrio-Hernandez

6. Sir Jeffrey Cheah Sunway Medical School, Faculty of Medical and Life Sciences, 24 Sunway University, Sunway City, Malaysia

   Gene Ching Chiek Koh

7. Department of Genomic Medicine, University of Cambridge, Cambridge, UK

   Gene Ching Chiek Koh

Contributions

A.R.B., M.J.G., T.B. and E.G. conceived and designed the study; A.R.B., M.J.G. and L-P.Z. supervised the work; T.B., L.D., R.S., E.S., M.S., A.B., G.C.C.K. performed and interpreted experiments; E.K., E.G., I.B-H. performed and interpreted computational analysis. T.B., I.B-H., E.K., E.G., A.R.B. drafted paper and all authors contributed to editing.

Corresponding author

Correspondence to Andrew R. Bassett.

Competing interests

A.B. is a founder of and consultant for Ensocell therapeutics. M.G. is a founder of and consultant for Mosaic Therapeutics, receives research funding from GSK and Astex Pharmaceuticals and is a consultant for Bristol-Myers Squibb. The remaining authors declare no competing interests.

Peer review information

Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work. A peer review file is available.

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