An oral Eimeria-vectored vaccine induces protective immunity against chicken infectious anemia

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An oral Eimeria-vectored vaccine induces protective immunity against chicken infectious anemia

References

  1. Blake, D. P. et al. Re-calculating the cost of coccidiosis in chickens. Vet. Res. 51, 115 (2020).

    Google Scholar 

  2. Chapman, H. D. et al. A selective review of advances in coccidiosis research. Adv. Parasitol. 83, 93–171 (2013).

    Google Scholar 

  3. Liu, Q., Liu, X., Zhao, X., Zhu, X. Q. & Suo, X. Live attenuated anticoccidial vaccines for chickens. Trends Parasitol. 39, 1087–1099 (2023).

    Google Scholar 

  4. Blake, D. P. & Tomley, F. M. Securing poultry production from the ever-present Eimeria challenge. Trends Parasitol. 30, 12–19 (2014).

    Google Scholar 

  5. Zhao, D. et al. Innovative prevention and control of coccidiosis: targeting sporogony for new control agent development. Poult. Sci. 103, 104246 (2024).

    Google Scholar 

  6. Kong, W. et al. Mucosal delivery of a self-destructing Salmonella-based vaccine inducing immunity against Eimeria. Avian Dis. 64, 254–268 (2020).

    Google Scholar 

  7. Tsai, C. J. Y., Loh, J. M. S., Fujihashi, K. & Kiyono, H. Mucosal vaccination: onward and upward. Expert Rev. Vaccines 22, 885–899 (2023).

    Google Scholar 

  8. Miquel-Clopés, A., Bentley, E. G., Stewart, J. P. & Carding, S. R. Mucosal vaccines and technology. Clin. Exp. Immunol. 196, 205–214 (2019).

    Google Scholar 

  9. Zhang, Z. et al. Mucosal immunity and vaccination strategies: current insights and future perspectives. Mol. Biomed. 6, 57 (2025).

    Google Scholar 

  10. Ravikumar, R., Chan, J. & Prabakaran, M. Vaccines against major poultry viral diseases: strategies to improve the breadth and protective efficacy. Viruses 14, 1195 (2022).

  11. Tang, X., Liu, X. & Suo, X. Towards innovative design and application of recombinant Eimeria as a vaccine vector. Infect. Immun. 88, e00861-19 (2020).

  12. Li, Y. et al. Genetic manipulation for the non-model protozoan Eimeria: Advancements, challenges, and future perspective. iScience 28, 112060 (2025).

    Google Scholar 

  13. Pastor-Fernández, I. et al. Laboratory growth and genetic manipulation of Eimeria tenella. Curr. Protoc. Microbiol 53, e81 (2019).

    Google Scholar 

  14. Huang, J. et al. Immune protection of microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens. Avian Pathol. 44, 392–400 (2015).

    Google Scholar 

  15. Marugan-Hernandez, V. et al. Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system. Parasit. Vectors 9, 463 (2016).

    Google Scholar 

  16. Davidson, I. et al. Chicken infectious anemia virus infection in Israeli commercial flocks: virus amplification, clinical signs, performance, and antibody status. Avian Dis. 48, 108–118 (2004).

    Google Scholar 

  17. Duan, Y. et al. Current knowledge on the diagnostic methods, epidemiological characteristics and antiviral strategies of chicken anemia virus. Vet. Sci. 12, 1154 (2025).

  18. Wang, H., Dan, Y., Li, L. & Wang, X. Advances in chicken infectious anemia vaccines. Vaccines 13, 277 (2025).

  19. Meehan, B. M., Todd, D., Creelan, J. L., Connor, T. J. & McNulty, M. S. Investigation of the attenuation exhibited by a molecularly cloned chicken anemia virus isolate by utilizing a chimeric virus approach. J. Virol. 71, 8362–8367 (1997).

    Google Scholar 

  20. Zhang, X. et al. Assessing the efficacy of an inactivated chicken anemia virus vaccine. Vaccine 33, 1916–1922 (2015).

    Google Scholar 

  21. Liu, X. et al. Chicken anemia virus VP1 negatively regulates type I interferon via targeting interferon regulatory factor 7 of the DNA-sensing pathway. Poult. Sci. 102, 102291 (2023).

    Google Scholar 

  22. Chen, J. et al. Chicken infectious anemia virus (CIAV) VP1 antagonizes type I interferon (IFN-I) production by inhibiting TBK1 phosphorylation. Virus Res. 327, 199077 (2023).

    Google Scholar 

  23. Rosario, K. et al. Revisiting the taxonomy of the family Circoviridae: establishment of the genus Cyclovirus and removal of the genus Gyrovirus. Arch. Virol. 162, 1447–1463 (2017).

    Google Scholar 

  24. Dai, M. et al. Genomic sequence and pathogenicity of the chicken anemia virus isolated from chicken in Yunnan Province, China. Front. Vet. Sci. 9, 860134 (2022).

    Google Scholar 

  25. Shirley, M. W., Smith, A. L. & Tomley, F. M. The biology of avian Eimeria with an emphasis on their control by vaccination. Adv. Parasitol. 60, 285–330 (2005).

    Google Scholar 

  26. Pallesen, J. et al. Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen. Proc. Natl. Acad. Sci. USA 114, E7348–e7357 (2017).

    Google Scholar 

  27. Chapman, H. D. Milestones in avian coccidiosis research: a review. Poult. Sci. 93, 501–511 (2014).

    Google Scholar 

  28. Chapman, H. D. et al. Sustainable coccidiosis control in poultry production: the role of live vaccines. Int. J. Parasitol. 32, 617–629 (2002).

    Google Scholar 

  29. Johnson, J. & Reid, W. M. Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp. Parasitol. 28, 30–36 (1970).

    Google Scholar 

  30. Zhang, S. et al. Establishment of recombinant eimeria acervulina expressing multi-copies M2e derived from avian influenza virus H9N2. Vaccines 9, 791 (2021).

  31. Yu, Y. et al. Microneme-located VP2 in Eimeria acervulina elicits effective protective immunity against infectious bursal disease virus. Infect. Immun. 92, e0045623 (2024).

    Google Scholar 

  32. Chen, W. et al. Precocious Eimeria magna transgenically expressing RHDV P2 subdomain induces immune responses in rabbits. npj Vaccines 10, 167 (2025).

    Google Scholar 

  33. Clark, J. D. et al. Eimeria species parasites as novel vaccine delivery vectors: anti-Campylobacter jejuni protective immunity induced by Eimeria tenella-delivered CjaA. Vaccine 30, 2683–2688 (2012).

    Google Scholar 

  34. Tang, X. et al. Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice. Sci. Rep. 6, 29379 (2016).

    Google Scholar 

  35. Tang, X. et al. A novel vaccine delivery model of the apicomplexan eimeria tenella expressing eimeria maxima antigen protects chickens against infection of the two parasites. Front. Immunol. 8, 1982 (2017).

    Google Scholar 

  36. Pastor-Fernández, I. et al. Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis. Parasit. Vectors 13, 343 (2020).

    Google Scholar 

  37. Yan, W. et al. Stable transfection of Eimeria tenella: constitutive expression of the YFP-YFP molecule throughout the life cycle. Int. J. Parasitol. 39, 109–117 (2009).

    Google Scholar 

  38. Yin, G., Liu, X., Zou, J., Huang, X. & Suo, X. Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy. Int. J. Parasitol. 41, 813–816 (2011).

    Google Scholar 

  39. Liu, J. et al. Evidence of high-efficiency cross fertilization in Eimeria acervulina revealed using two lines of transgenic parasites. Int. J. Parasitol. 53, 81–89 (2023).

    Google Scholar 

  40. Liu, L. et al. DNA prime and recombinant protein boost vaccination confers chickens with enhanced protection against chicken infectious anemia virus. Viruses 14, 2115 (2022).

  41. Shen, S. Y. et al. Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ. Vet. Immunol. Immunopathol. 167, 200–204 (2015).

    Google Scholar 

  42. Ge, C. et al. Recombinant Marek’s disease virus expressing VP1 and VP2 proteins provides robust immune protection against chicken infectious anemia virus. Front. Microbiol. 15, 1515415 (2024).

    Google Scholar 

  43. Koch, G., van Roozelaar, D. J., Verschueren, C. A., van der Eb, A. J. & Noteborn, M. H. Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13, 763–770 (1995).

    Google Scholar 

  44. Schat, K. A., Martins, N. R., O’Connell, P. H. & Piepenbrink, M. S. Immune complex vaccines for chicken infectious anemia virus. Avian Dis. 55, 90–96 (2011).

    Google Scholar 

  45. Morgan, B. P., Marchbank, K. J., Longhi, M. P., Harris, C. L. & Gallimore, A. M. Complement: central to innate immunity and bridging to adaptive responses. Immunol. Lett. 97, 171–179 (2005).

    Google Scholar 

  46. Nielsen, C. H. & Leslie, R. G. Complement’s participation in acquired immunity. J. Leukoc. Biol. 72, 249–261 (2002).

    Google Scholar 

  47. Rickert, R. C. Regulation of B lymphocyte activation by complement C3 and the B cell coreceptor complex. Curr. Opin. Immunol. 17, 237–243 (2005).

    Google Scholar 

  48. Dempsey, P. W., Allison, M. E., Akkaraju, S., Goodnow, C. C. & Fearon, D. T. C3d of complement as a molecular adjuvant: bridging innate and acquired immunity. Science 271, 348–350 (1996).

    Google Scholar 

  49. Vassalakis, J. A. et al. Murine C3 of the complement system affects infection by Leptospira interrogans. Microbes Infect. 27, 105413 (2025).

    Google Scholar 

  50. Qi, X. et al. The C3d-fused Porcine circovirus type 2d virus-like particle induced early and enhanced immune response and protected pigs against challenge. Vet. Microbiol. 299, 110305 (2024).

    Google Scholar 

  51. Galvez-Romero, G., Salas-Rojas, M., Pompa-Mera, E. N., Chávez-Rueda, K. & Aguilar-Setién, Á Addition of C3d-P28 adjuvant to a rabies DNA vaccine encoding the G5 linear epitope enhances the humoral immune response and confers protection. Vaccine 36, 292–298 (2018).

    Google Scholar 

  52. Li, Z. et al. Transgenic Eimeria mitis expressing chicken interleukin 2 stimulated higher cellular immune response in chickens compared with the wild-type parasites. Front. Microbiol. 6, 533 (2015).

    Google Scholar 

  53. Bussière, F. I. et al. Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development. Parasit. Vectors 11, 44 (2018).

    Google Scholar 

  54. Takano, A. et al. Detection of Eimeria oocysts in chicken feces using flotation recovery with sucrose or saturated saline solution. Acta Parasitol. 70, 17 (2025).

    Google Scholar 

  55. Long, P. L., Millard, B. J., Joyner, L. P. & Norton, C. C. A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Vet. Lat. 6, 201–217 (1976).

    Google Scholar 

  56. Schmatz, D. M., Crane, M. S. & Murray, P. K. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J. Protozool. 31, 181–183 (1984).

    Google Scholar 

  57. Duan, C. et al. Nucleofection and in vivo propagation of chicken eimeria parasites. J Vis Exp 156, e60552 (2020).

  58. Wang, L. et al. Comparative analysis of model antigen expression level driven by the microneme protein promoters in Eimeria tenella: High-Expression Level Microneme Protein Promoter. Poult. Sci. 104, 105600 (2025).

    Google Scholar 

  59. Liu, X. et al. Restriction enzyme-mediated transfection improved transfection efficiency in vitro in Apicomplexan parasite Eimeria tenella. Mol. Biochem. Parasitol. 161, 72–75 (2008).

    Google Scholar 

  60. Sun, P. et al. EtcPRS (Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii. iScience 26, 106334 (2023).

    Google Scholar 

  61. Clark, J. D. et al. A toolbox facilitating stable transfection of Eimeria species. Mol. Biochem. Parasitol. 162, 77–86 (2008).

    Google Scholar 

  62. Jeffers, T. K. Attenuation of Eimeria tenella through selection for precociousness. J. Parasitol. 61, 1083–1090 (1975).

    Google Scholar 

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