Artificial intelligence-assisted optimization of Lentinula edodes extracts for enhanced bioactive profile and therapeutic potential

Posted on
artificial-intelligence-assisted-optimization-of-lentinula-edodes-extracts-for-enhanced-bioactive-profile-and-therapeutic-potential
Artificial intelligence-assisted optimization of Lentinula edodes extracts for enhanced bioactive profile and therapeutic potential

References

  1. Das, A. et al. Medicinal components in edible mushrooms on diabetes mellitus treatment. Pharmaceutics 14, 436 (2022).

    Google Scholar 

  2. Sevindik, M., Bal, C., Eraslan, E. C., Uysal, I. & Mohammed, F. S. Medicinal mushrooms: a comprehensive study on their antiviral potential. Prospects Pharm. Sci. 21, 42–56 (2023).

    Google Scholar 

  3. Gupta, K., Tejaswini, M. & Umekar, M. J. Medicinal Value of Mushroom: A Deeper Insight. Curr. Indian Sci. 2, e2210299X2285895 (2024).

    Google Scholar 

  4. Maeng, J. H., Shahbaz, M., Ameer, H., Jo, K., Kwon, J. H. & Y. & Optimization of microwave-assisted extraction of bioactive compounds from Coriolus versicolor mushroom using response surface methodology. J. Food Process Eng. 40, e12421 (2017).

    Google Scholar 

  5. Taofiq, O. et al. Optimization of ergosterol extraction from Pleurotus mushrooms using response surface methodology. Food Funct. 11, 5887–5897 (2020).

    Google Scholar 

  6. Gürgen, A. & Sevindik, M. Application of artificial neural network coupling multiobjective particle swarm optimization algorithm to optimize Pleurotus ostreatus extraction parameters. J. Food Process. Preserv. 46, e16949 (2022).

    Google Scholar 

  7. Sasaki, S. H., Linhares, R. E., Nozawa, C. M., Montalván, R. & Paccola-Meirelles, L. D. Strains of Lentinula edodes suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of vesicular stomatitis virus. Brazilian J. Microbiol. 32, 52–55 (2001).

    Google Scholar 

  8. Nikitina, V. E., Tsivileva, O. M., Pankratov, A. N. & Bychkov, N. A. Lentinula edodes biotechnology–from lentinan to lectins. Food Technol. Biotechnol. 45, 230–237 (2007).

    Google Scholar 

  9. Finimundy, T. C., Dillon, A. J. P., Henriques, J. A. P. & Ely, M. R. A review on general nutritional compounds and pharmacological properties of the Lentinula edodes mushroom. Food Nutr. Sciences 2014 (2014).

  10. Ponnusamy, C. et al. Lentinula edodes (edible mushroom) as a nutraceutical: a review. Biosci. Biotechnol. Res. Asia. 19, 1–11 (2022).

    Google Scholar 

  11. Xiang, Q. et al. in Advances in Plant Breeding Strategies: Vegetable Crops: Volume 10: Leaves, Flowerheads, Green Pods, Mushrooms and Truffles 443–476 (Springer, 2021).

  12. Song, X. et al. Cultivation methods and biology of Lentinula edodes. Appl. Microbiol. Biotechnol. 109, 1–15 (2025).

    Google Scholar 

  13. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37, 277–285 (2004).

    Google Scholar 

  14. Erel, O. A new automated colorimetric method for measuring total oxidant status. Clin. Biochem. 38, 1103–1111 (2005).

    Google Scholar 

  15. Sevindik, M. Anticancer, antimicrobial, antioxidant and DNA protective potential of mushroom Leucopaxillus gentianeus (Quél.) Kotl. Indian J. Experimental Biology (IJEB). 59, 310–315 (2021).

    Google Scholar 

  16. Ünal, O. et al. Optimization of Phellinus hartigii extracts: Biological activities, and phenolic content analysis. BMC Complement. Med. Ther. 25, 113 (2025).

    Google Scholar 

  17. Ellman, G. L., Courtney, K. D., Andres Jr, V. & Featherstone, R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95 (1961).

    Google Scholar 

  18. Sultana, S. et al. Assessment of proximate composition, mineral element profile and antioxidant properties of the edible oyster mushroom grown in Bangladesh. Discover Food. 4, 126 (2024).

    Google Scholar 

  19. Kitzberger, C. S. G., Smânia Jr, A., Pedrosa, R. C. & Ferreira, S. R. S. Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J. Food Eng. 80, 631–638 (2007).

    Google Scholar 

  20. Sasidharan, S. et al. In vitro antioxidant activity and hepatoprotective effects of Lentinula edodes against paracetamol-induced hepatotoxicity. Molecules 15, 4478–4489 (2010).

    Google Scholar 

  21. Ren, Z. et al. Antioxidant and anti-inflammation of enzymatic-hydrolysis residue polysaccharides by Lentinula edodes. Int. J. Biol. Macromol. 120, 811–822 (2018).

    Google Scholar 

  22. Xiang, Q. et al. Enhanced effects of iron on mycelial growth, metabolism and in vitro antioxidant activity of polysaccharides from Lentinula edodes. Bioengineering 9, 581 (2022).

    Google Scholar 

  23. Sevindik, M., Gürgen, A., Korkmaz, A. F. & Akata, I. Optimizing Ultrasonic-Assisted Extraction Process of Paralepista flaccida: A Comparative Study of Antioxidant, Anticholinesterase, and Antiproliferative Activities via Response Surface Methodology and Artificial Neural Network Modeling. Molecules 30, 3317 (2025).

    Google Scholar 

  24. Gürgen, A. & Sevindik, M. Single and Multi-Objective Optimization of the Red Pine Mushroom Lactarius deliciosus (Agaricomycetes) Extraction Conditions Using Artificial Intelligence Methods and Biological Activities of Optimized Extracts. Int. J. Med. Mushrooms. 27, 59–73 (2025).

    Google Scholar 

  25. Sevindik, M., Gürgen, A., Khassanov, V. T. & Bal, C. Biological activities of ethanol extracts of Hericium erinaceus obtained as a result of optimization analysis. Foods 13, 1560 (2024).

    Google Scholar 

  26. Gürgen, A., Unal, O. & Sevindik, M. Biological activities of the golden chantarelle mushroom Cantharellus cibarius (Agaricomycetes) extracts obtained as a result of single and multi-objective optimization studies. Int. J. Med. Mushrooms. 26, 63–74 (2024).

    Google Scholar 

  27. Trobec, T. et al. Wild Mushrooms as a Source of Cholinesterase and Glutathione S-Transferase Inhibitors. International J. Med. Mushrooms 27, 23-34 (2025).

  28. Lee, M. R. et al. Comparison of constituents, antioxidant potency, and acetylcholinesterase inhibition in Lentinus edodes, Sparassis crispa, and Mycoleptodonoides aitchisonii. Food Sci. Biotechnol. 22, 1747–1751 (2013).

    Google Scholar 

  29. Wasser, S. P. Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: a review. International J. Med. mushrooms 19, 279-317 (2017).

  30. Sevindik, M. et al. The Role of Medicinal Mushrooms in Cancer Treatment: Bioactive Compounds and Therapeutic Potential. Int. J. Med. Mushrooms. 27, 1–24 (2025).

    Google Scholar 

  31. Israilides, C. et al. In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes. Phytomedicine 15, 512–519 (2008).

    Google Scholar 

  32. Arsianti, A. & Phytochemical Analysis Antioxidant Activity, and Cytotoxic Activity of Lentinula Edodes (Shiitake Mushroom) Extracts towards Breast Cancer Cell Line T47D. International J. Sci. Res. (IJSR) 12, 2044-2049 (2023).

  33. Qaicy, A. G. S., Samarrai, F. A., Haddad, M. F., Al-Tamer, Y. Y. & Anticancer Apoptotic, and Mitotic Effectiveness of Lentinula edodes Microwave-Assisted Extract against Certain 2D Monolayer Culture Cancer Cell Lines. Med. J. Babylon. 22, 195–200 (2025).

    Google Scholar 

  34. Zhou, Y. et al. A comprehensive review on phytochemical profiling in mushrooms: occurrence, biological activities, applications and future prospective. Food Reviews Int. 40, 924–951 (2024).

    Google Scholar 

  35. Nam, M., Choi, J. Y. & Kim, M. S. Metabolic profiles, bioactive compounds, and antioxidant capacity in Lentinula edodes cultivated on log versus sawdust substrates. Biomolecules 11, 1654 (2021).

    Google Scholar 

  36. Erdoğan Eliuz, E. A. Antibacterial activity and antibacterial mechanism of ethanol extracts of Lentinula edodes (Shiitake) and Agaricus bisporus (button mushroom). Int. J. Environ. Health Res. 32, 1828–1841 (2022).

    Google Scholar 

  37. Cui, L. et al. Identification of phytochemicals from Lentinus edodes and Auricularia auricula with UPLC-Q-Exactive Orbitrap MS. J. Future Foods. 2, 253–260 (2022).

    Google Scholar 

  38. Gaitan-Hernandez, R., Aquino-Bolaños, E. N., Herrera, M. & Salmones, D. Yield, and phenolic content of shiitake mushrooms cultivated on alternative substrates. Emirates J. Food Agric. 32, 188–197 (2020).

    Google Scholar 

  39. Ayar-Sümer, E. N., Verheust, Y., Özçelik, B. & Raes, K. Impact of lactic acid bacteria fermentation based on biotransformation of phenolic compounds and antioxidant capacity of mushrooms. Foods 13, 1616 (2024).

    Google Scholar 

  40. Baptista, F. et al. Nutraceutical potential of Lentinula edodes’ spent mushroom substrate: A comprehensive study on phenolic composition, antioxidant activity, and antibacterial effects. J. Fungi. 9, 1200 (2023).

    Google Scholar 

  41. Akter, A. et al. Comparative extraction of bioactive compounds from spent mushroom substrates of Lentinula edodes and Pleurotus ostreatus using subcritical water and pressurized ethanol. Ind. Crops Prod. 235, 121750 (2025).

    Google Scholar 

  42. Jiménez-Moreno, N., Volpe, F., Moler, J. A., Esparza, I. & Ancín-Azpilicueta, C. Impact of extraction conditions on the phenolic composition and antioxidant capacity of grape stem extracts. Antioxidants 8, 597 (2019).

    Google Scholar 

  43. Terpinc, P. et al. Maximizing the recovery of phenolic antioxidants from wild strawberry (Fragaria vesca) leaves using microwave-assisted extraction and accelerated solvent extraction. Processes 11, 3378 (2023).

    Google Scholar 

Download references

Related Posts