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The Synthetic Yeast Genome Project (Sc2.0) set out to redesign and chemically synthesize an entire eukaryotic genome. This Comment summarizes the design- and construction-related defects revealed during the construction of 16 synthetic chromosomes, and the solutions applied, drawing out the key biological and technical insights that will inform future genome-scale engineering.
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Acknowledgements
Our Synthetic Yeast Genome Project (Sc2.0) work was supported by the Australian Research Council Centre of Excellence in Synthetic Biology and by external grants from Bioplatforms Australia, the New South Wales (NSW) Chief Scientist and Engineer, and the NSW Government’s Department of Primary Industries. Figures and tables were edited and refined by Bronte Turner of Serpentine Studio.
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Erpf, P.E., Meier, F., Walker, R.S.K. et al. Building synthetic chromosomes one yeast at a time: insights from Sc2.0. Nat Biotechnol (2025). https://doi.org/10.1038/s41587-025-02913-4
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DOI: https://doi.org/10.1038/s41587-025-02913-4
