Mitchell, J. F. & Leopold, D. A. The marmoset monkey as a model for visual neuroscience. Neurosci. Res. 93, 20–46 (2015). Article PubMed PubMed Central […]
Category: Efficient
Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR)
References Pickar-Oliver, A. & Gersbach, C. A. The next generation of CRISPR-Cas technologies and applications. Nat. Rev. Mol. Cell Biol. 20, 490–507 (2019). Google Scholar […]
Efficient cellular transformation via protein delivery through the protrusion-derived extracellular vesicles
Introduction Extracellular vesicles (EVs) are lipid membrane-bound vesicles secreted by cells, facilitating the transfer of non-secreting components, including proteins, RNAs, lipids, and other cellular materials, […]
An efficient and reproducible cryopreservation protocol for sustainable conservation of Jojoba (Simmondsia chinensis)
References Dyer, J. M., Stymne, S., Green, A. G. & Carlsson, A. S. High value oils from plants. Plant. J. 54, 640–655 (2008). Google Scholar […]
Efficient synthesis of aqueous soluble Silymarin nanocrystals from Silybum marianum and assessment of their antibacterial and cytotoxicity insights
Introduction Silybum marianum, generally named Milk Thistle, is a botanical species belonging to the genus Leucanthemum1. Silybum marianum, as an herb, facilitates the process of […]
Efficient denitrification and N2O mitigation in low-C/N wastewater treatment by promoting TCA cycle anaplerosis via glyoxylate shunt regulation
Steffen, W. et al. Planetary boundaries: guiding human development on a changing planet. Science 347, 1259855 (2015). Article PubMed Google Scholar Liu, T. et al. […]
Efficient extracellular expression and rapid screening of human-like recombinant gelatin in Komagataella phaffii
Introduction Natural gelatin (Gel) is a collagen degradation product extracted from animal skin, bones, and connective tissues. Therefore, it shares a common origin with collagen […]
Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes
Introduction A dream of synthetic biologists working on complex multi-cellular organisms is to be able to reach beyond the manipulation of one or a few […]