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Category: Engineering
Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos
Introduction The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) systems as prokaryotes’ adaptive immune systems against invading phages or plasmids have […]
Engineering a single-chain immunoglobulin scaffold loaded with a latent-releasable cytotoxic pore-forming peptide
Introduction Pore-forming peptides (PFPs)1, a.k.a. anti-microbial peptides (AMPs)2,3 or membrane-permeabilizing peptides (MPPs)4, can be defined as ubiquitous small polypeptides (~10–40 residues) that interact with and […]
Engineering of an Fc-specific monovalent protein G for the light-controlled affinity purification of antibodies
Introduction Bacterial immunoglobulin (Ig)-binding proteins, in particular protein A from Staphylococcus aureus1 and protein G from group G Streptococci2, are widely used for the affinity […]
Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-making
Introduction Synthetic genetic circuits facilitate the reprogramming of cells to perform myriad functions, which has enabled developments and applications that span biotechnology, chemical biology, and […]
Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery
Introduction Transient delivery vehicles have garnered substantial attention in biomedicine, showcasing their efficacy in applications ranging from vaccines to gene therapy1,2,3. These vehicles offer a […]
Engineering of a bespoke CRISPR base editor to treat a severe vascular disease
Research Briefing Published: 15 September 2025 Nature Biomedical Engineering (2025)Cite this article Subjects We developed a customized base editing strategy to efficiently, precisely and safely […]
Engineering kidney developmental trajectory using culture boundary conditions
Introduction Branching morphogenesis is a common developmental program for epithelial organs to acquire a tree-like structure. This massively parallelizes their function because branch tips become […]
Engineering B cells to treat and study human disease
References Ueda, N. et al. Immunotherapy perspectives in the new era of B-cell editing. Blood Adv. 5, 1770–1779 (2021). CAS Google Scholar Edelstein, J., Fritz, […]
In vivo engineering broadens CAR-T cell therapy use
RESEARCH HIGHLIGHT 05 August 2025 Chimeric antigen receptor (CAR)-T cell therapies have transformed the treatment of B cell malignancies, offering durable responses. However, their broader […]