Main The precise and targeted integration of transgenes using CRISPR–Cas technology holds great promise for applications in biotechnology and gene therapy1. However, it is paramount […]
Category: Precise
Precise mapping of single-stranded DNA breaks by sequence-templated erroneous DNA polymerase end-labelling
Introduction Cellular DNA is constantly subjected to threats, both from the environment via exposure to radiation or chemicals and as a consequence of natural cellular […]
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens
Mayorga-Ramos, A., Zúñiga-Miranda, J., Carrera-Pacheco, S. E., Barba-Ostria, C. & Guamán, L. P. CRISPR-Cas-based antimicrobials: design, challenges, and bacterial mechanisms of resistance. ACS Infect. Dis. […]