Differential effects of biologically and chemically synthesized copper oxide nanoparticles on artemisinin biosynthesis gene expression in Artemisia absinthium

differential-effects-of-biologically-and-chemically-synthesized-copper-oxide-nanoparticles-on-artemisinin-biosynthesis-gene-expression-in-artemisia-absinthium
Differential effects of biologically and chemically synthesized copper oxide nanoparticles on artemisinin biosynthesis gene expression in Artemisia absinthium

References

  1. Batiha, G. E. S. et al. Bioactive compounds, Pharmacological actions, and pharmacokinetics of Wormwood (Artemisia absinthium). Antibiotics 9, 353 (2020).

    Google Scholar 

  2. Bhat, R. R. et al. Springer, Cham,. Chemical Composition and Biological Uses of Artemisia absinthium (Wormwood). in Plant and Human Health Vol. 3 (ed M. Ozturk, Hakeem, K.) 37–63 (2019).

  3. Negri, S. et al. Bioprospecting of Artemisia genus: from Artemisinin to other potentially bioactive compounds. Sci. Rep. 14, 4791 (2024).

    Google Scholar 

  4. Li, Y. et al. Advanced metabolic engineering strategies for increasing Artemisinin yield in Artemisia annua L. Hortic. Res. 11, uhad292 (2024).

    Google Scholar 

  5. Qamar, F. et al. Increased Artemisinin production in Artemisia annua L. by co-overexpression of six key biosynthetic enzymes. Int. J. Biol. Macromol. 281, 136291 (2024).

    Google Scholar 

  6. Gunasena, M. et al. Transforming plant tissue culture with nanoparticles: A review of current applications. Plant Nano Biol. 100102 (2024).

  7. Murthy, H. N., Joseph, K. S., Paek, K. Y. & Park, S. Y. Nanomaterials as novel elicitors of Pharmacologically active plant specialized metabolites in cell and organ cultures: current status and future outlooks. Plant. Growth Regul. 104, 5–30 (2024).

    Google Scholar 

  8. Espinosa-Leal, C. A., Puente-Garza, C. A. & García-Lara Vitro plant tissue culture: means for production of biological active compounds. Planta 248, 1–18 (2018).

    Google Scholar 

  9. Muangphrom, P., Seki, H., Fukushima, E. O. & Muranaka, T. Artemisinin-based antimalarial research: application of biotechnology to the production of artemisinin, its mode of action, and the mechanism of resistance of Plasmodium parasites. J. Nat. Med. 70, 318–334 (2016).

    Google Scholar 

  10. Zhao, L. et al. From plant to Yeast—Advances in biosynthesis of Artemisinin. Molecules 27, 6888 (2022).

    Google Scholar 

  11. Ranjbar, M., Naghavi, M. R., Alizadeh, H. & Soltanloo, H. Expression of Artemisinin biosynthesis genes in eight Artemisia species at three developmental stages. Ind. Crops Prod. 76, 836–843 (2015).

    Google Scholar 

  12. Yuan, M. et al. AaMYC3 bridges the regulation of glandular trichome density and Artemisinin biosynthesis in Artemisia annua. Plant. Biotechnol. J. 23, 315–332 (2025).

    Google Scholar 

  13. Jan, S., Jan, N., Singh, S. & Shah, M. A. & Bhat, I. A. Nanotechnology in plant tissue culture: A review. Hortic. Plant. J. (2025).

  14. Molahalli, V. et al. American Chemical Society,. Properties, synthesis, and characterization of cu-based nanomaterials. in Copper-Based Nanomaterials in Organic Transformations Vol. 1466 (ed A. Srivastava, Srivastava, A.) Ch. Chapter 1, 1–33 (2024).

  15. Mawale, K. S., Nandini, B. & Giridhar, P. Copper and silver nanoparticle seed priming and foliar spray modulate plant growth and thrips infestation in Capsicum spp. ACS Omega. 9, 3430–3444 (2024).

    Google Scholar 

  16. Perfilieva, A., Sukhov, B., Kon’kova, T., Strekalovskaya, E. & Krutovsky, K. Diversity of copper-containing nanoparticles and their influence on plant growth and development. Plant Physiol. Biochem. 109575 (2025).

  17. Singh, D., Sharma, A., Verma, S. K., Pandey, H. & Pandey, M. Impact of nanoparticles on plant physiology, nutrition, and toxicity: A short review. Next Nanatechnol. 6, 100081 (2024).

    Google Scholar 

  18. Kolahalam, L. A., Prasad, K., Krishna, P. M., Supraja, N. & Shanmugan, S. The exploration of bio-inspired copper oxide nanoparticles: synthesis, characterization and in-vitro biological investigations. Heliyon 8 (2022).

  19. Havryliuk, O. et al. Unveiling the potential of CuO and Cu2O nanoparticles against novel copper-resistant Pseudomonas strains: an in-depth comparison. Nanomaterials 14, 1644 (2024).

    Google Scholar 

  20. Čarapar, I. et al. The metal oxidation state in Cu, CuO, and Cu2O nanoparticles plays a key role in toxicity to sea urchin arbacia lixula, paracentrotus lividus, and Sphaerechinus granularis embryos. Toxics 13, 469 (2025).

    Google Scholar 

  21. Mahjouri, S., Movafeghi, A., Divband, B. & Kosari-Nasab, M. Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of Nicotiana tabacum. Plant. Cell. Tissue Organ. Cult. 135, 223–234 (2018).

    Google Scholar 

  22. Jafarirad, S., Kosari–Nasab, M., Tavana, R. M., Mahjouri, S. & Ebadollahi, R. Impacts of manganese bio-based nanocomposites on phytochemical classification, growth and physiological responses of Hypericum perforatum L. shoot cultures. Ecotoxicol. Environ. Saf. 209, 111841 (2021).

    Google Scholar 

  23. Ying, S. et al. Green synthesis of nanoparticles: current developments and limitations. Environ. Technol. Innov. 26, 102336 (2022).

    Google Scholar 

  24. Din, M. I., Arshad, F., Hussain, Z. & Mukhtar, M. Green adeptness in the synthesis and stabilization of copper nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and antioxidant activities. Nanoscale Res. Lett. 12, 638 (2017).

    Google Scholar 

  25. Jafarirad, S., Rasoulpour, I., Divband, B., Hammami Torghabe, I. & Kosari-Nasab, M. Innovative biocapped CuO nano-photocatalysts: a rapid and green method for photocatalytic degradation of 4-nitrophenol. Mater. Res. Innov. 22, 415–421 (2018).

    Google Scholar 

  26. Khatoon, U. T., Velidandi, A. & Rao, G. N. Copper oxide nanoparticles: synthesis via chemical reduction, characterization, antibacterial activity, and possible mechanism involved. Inorg. Chem. Commun. 149, 110372 (2023).

    Google Scholar 

  27. Zhang, Y. et al. Reference genes screening and gene expression patterns analysis involved in gelsenicine biosynthesis under different hormone treatments in Gelsemium elegans. Int. J. Mol. Sci. 24, 15973 (2023).

    Google Scholar 

  28. Nassar, A. R. A., Atta, H. M., Abdel-Rahman, M. A., Naghy, E., Fouda, A. & W. S. & Myco-synthesized copper oxide nanoparticles using Harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities. BMC Complement. Med. Ther. 23, 261 (2023).

    Google Scholar 

  29. Olofsson, L., Engström, A., Lundgren, A. & Brodelius, P. E. Relative expression of genes of terpene metabolism in different tissues of Artemisia annua L. BMC plant. biol. 11, 1–12 (2011).

    Google Scholar 

  30. Salehi, M., Karimzadeh, G., Naghavi, M. R. & Badi, N. Rashidi Monfared, S. Expression of key genes affecting Artemisinin content in five Artemisia species. Sci. Rep. 8, 12659 (2018).

    Google Scholar 

  31. Ghassemi, B., Nayeri, F. D. & Hosseini, R. The effects of Chitosan nanoparticles on genes expression of Artemisinin synthase in suspension culture of Artemisia annua L.: A comparative study. Int. J. Adv. Biol. Biomed. Res. 9, 190–203 (2021).

    Google Scholar 

  32. Zhang, B., Zheng, L. P., Li, Y., Wen Wang, J. & W. & Stimulation of Artemisinin production in Artemisia annua hairy roots by Ag-SiO2 core-shell nanoparticles. Curr. Nanosci. 9, 363–370 (2013).

    Google Scholar 

  33. Caretto, S. et al. Methyl jasmonate and miconazole differently affect arteminisin production and gene expression in Artemisia annua suspension cultures. Plant. Biol. 13, 51–58 (2011).

    Google Scholar 

  34. Zhou, L. et al. Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L. Front. Med. 11, 137–146 (2017).

    Google Scholar 

  35. Hayat, K., Ali, S., Ullah, S., Fu, Y. & Hussain, M. Green synthesized silver and copper nanoparticles induced changes in biomass parameters, secondary metabolites production, and antioxidant activity in callus cultures of Artemisia absinthium L. Green. Process. Synth. 10, 61–72 (2021).

    Google Scholar 

  36. Shi, P. et al. Promotion of Artemisinin content in Artemisia annua by overexpression of multiple Artemisinin biosynthetic pathway genes. Plant. Cell. Tissue Organ. Cult. 129, 251–259 (2017).

    Google Scholar 

  37. Chen, Y. et al. The stacked over-expression of FPS, CYP71AV1 and CPR genes leads to the increase of Artemisinin level in Artemisia annua L. Plant. Biotechnol. Rep. 7, 287–295 (2013).

    Google Scholar 

  38. khaldari, I., Naghavi, M. R., Motamedi, E. & Zargar, M. The effects of green and chemically-synthesized copper oxide nanoparticles on the production and gene expression of morphinan alkaloids in Oriental poppy. Sci. Rep. 14, 6000 (2024).

    Google Scholar 

  39. Hazrati, R., Zare, N., Asghari, R., Sheikhzadeh, P. & Johari-Ahar, M. Biologically synthesized CuO nanoparticles induce physiological, metabolic, and molecular changes in the Hazel cell cultures. Appl. Microbiol. Biotechnol. 106, 6017–6031 (2022).

    Google Scholar 

  40. Tripathi, D., Rai, K. K. & Pandey-Rai, S. Impact of green synthesized WcAgNPs on in-vitro plant regeneration and withanolides production by inducing key biosynthetic genes in Withania coagulans. Plant. Cell. Rep. 40, 283–299 (2021).

    Google Scholar 

  41. Tripathi, D., Goswami, N. K. & Pandey-Rai, S. Interactive effect of biogenic nanoparticles and UV-B exposure on physio-biochemical behavior and secondary metabolism of Artemisia annua L. Plant. Nano Biol. 10, 100097 (2024).

    Google Scholar 

  42. Ghasemi, B., Hosseini, R. & Nayeri, F. D. Effects of Cobalt nanoparticles on Artemisinin production and gene expression in Artemisia annua. Turk. J. Bot. 39, 769–777 (2015).

    Google Scholar 

  43. Ayoobi, A., Asgarani, E. & Efferth, T. Iron oxide nanoparticle (Fe3O4-NP) elicitation of Artemisia annua L. in vitro toward enhancing artemisinin production through overexpression of key genes of terpenoids biosynthesis pathway and induction of oxidative stress. (2023).

  44. Cao, J. et al. Graphene enhances Artemisinin production in the traditional medicinal plant artemisia annua via dynamic physiological processes and MiRNA regulation. Plant Commun. 5 (2024).

Download references