References
-
Klug, L. & Daum, G. Yeast lipid metabolism at a glance. FEMS Yeast Res. 14, 369–388 (2014).
-
Mbuyane, L. L., Bauer, F. F. & Divol, B. The metabolism of lipids in yeasts and applications in oenology. Food Res. Int. 141, 110142 (2021).
-
Tesnière, C. Importance and role of lipids in wine yeast fermentation. Appl. Microbiol. Biotechnol. 103, 8293–8300 (2019).
-
Belviso, S., Bardi, L., Bartolini, A. B. & Marzona, M. Lipid nutrition of Saccharomyces cerevisiae in winemaking. Can. J. Microbiol. 50, 669–674 (2004).
-
Casalta, E. et al. Impact of phytosterol addition on fermentation progress and volatile compounds synthesis during alcoholic fermentation in synthetic and natural grape musts. OENO One 57, 41–52 (2023).
-
Casu, F., Pinu, F. R., Fedrizzi, B., Greenwood, D. R. & Villas-Boas, S. G. The effect of linoleic acid on the Sauvignon blanc fermentation by different wine yeast strains. FEMS Yeast Res. 16, fow050 (2016).
-
Luparia, V., Soubeyrand, V., Berges, T., Julien, A. & Salmon, J.-M. Assimilation of grape phytosterols by Saccharomyces cerevisiae and their impact on enological fermentations. Appl Microbiol Biotechnol 65, 25–32 (2004).
-
Piva, G. G. et al. Unveiling the power of adding sterols in wine: Optimizing alcoholic fermentation with strategic management. Int. J. Food Microbiol. 406, 110350 (2023).
-
Piva, G. G. et al. Role of Sterols During Wine Alcoholic Fermentation: Impact of the Type (Ergosterol and Phytosterols), Dose and Timing of Addition on Saccharomyces Cerevisiae Growth and Metabolism. SSRN Scholarly Paper at https://doi.org/10.2139/ssrn.4431557 (2023).
-
Varela, C., Torrea, D., Schmidt, S. A., Ancin-Azpilicueta, C. & Henschke, P. A. Effect of oxygen and lipid supplementation on the volatile composition of chemically defined medium and Chardonnay wine fermented with Saccharomyces cerevisiae. Food Chem. 135, 2863–2871 (2012).
-
Tumanov, S. et al. Comprehensive lipidome profiling of Sauvignon blanc grape juice. Food Chem. 180, 249–256 (2015).
-
Van der Rest, M. E. et al. The plasma membrane of Saccharomyces cerevisiae: structure, function, and biogenesis. Microbiol Rev. 59, 304–322 (1995).
-
Hamilton, J. A. Fatty acid transport: difficult or easy? J. Lipid Res. 39, 467–481 (1998).
-
Zakim, D. Thermodynamics of fatty acid transfer. J. Membr. Biol. 176, 101–109 (2000).
-
Dulermo, R., Gamboa-Meléndez, H., Dulermo, T., Thevenieau, F. & Nicaud, J.-M. The fatty acid transport protein Fat1p is involved in the export of fatty acids from lipid bodies in Yarrowia lipolytica. FEMS Yeast Res. 14, 883–896 (2014).
-
Zou, Z., DiRusso, C. C., Ctrnacta, V. & Black, P. N. Fatty Acid Transport in Saccharomyces cerevisiae: directed mutagenesis of Fat1 distinguishes the biochemical activities associated with Fat1p *. J. Biol. Chem. 277, 31062–31071 (2002).
-
Deytieux, C., Mussard, L., Biron, M.-J. & Salmon, J.-M. Fine measurement of ergosterol requirements for growth of Saccharomyces cerevisiae during alcoholic fermentation. Appl. Microbiol. Biotechnol. 68, 266–271 (2005).
-
Duan, L. L. et al. Effects of adding unsaturated fatty acids on fatty acid composition of saccharomyces cerevisiae and major volatile compounds in wine. South Afr. J. Enol. Vitic. 36, 285–295 (2015).
-
Rodriguez, R. J., Low, C., Bottema, C. D. K. & Parks, L. W. Multiple functions for sterols in Saccharomyces cerevisiae. Biochim. Biophys. Acta (BBA) – Lipids Lipid Metab. 837, 336–343 (1985).
-
Girardi Piva, G. et al. Characterization and Role of Sterols in Saccharomyces cerevisiae during White Wine Alcoholic Fermentation. Fermentation 8, 90 (2022).
-
Pinu, F. R., Edwards, P. J. B., Gardner, R. C. & Villas-Boas, S. G. Nitrogen and carbon assimilation by Saccharomyces cerevisiae during Sauvignon blanc juice fermentation. FEMS Yeast Res. 14, 1206–1222 (2014).
-
Kohlwein, S. D. Analyzing and understanding lipids of yeast: a challenging endeavor. Cold Spring Harb. Protoc. 2017, pdb.top078956 (2017).
-
Ejsing, C. S. et al. Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry. Proc. Natl. Acad. Sci. 106, 2136–2141 (2009).
-
Aguilera, F., Peinado, R. A., Millán, C., Ortega, J. M. & Mauricio, J. C. Relationship between ethanol tolerance, H+-ATPase activity and the lipid composition of the plasma membrane in different wine yeast strains. Int. J. Food Microbiol. 110, 34–42 (2006).
-
Dong, S.-J., Yi, C.-F. & Li, H. Changes of Saccharomyces cerevisiae cell membrane components and promotion to ethanol tolerance during the bioethanol fermentation. Int. J. Biochem. Cell Biol. 69, 196–203 (2015).
-
You, K. M., Rosenfield, C.-L. & Knipple, D. C. Ethanol tolerance in the yeast Saccharomyces cerevisiae is dependent on cellular oleic acid content. Appl Environ. Microbiol. 69, 1499–1503 (2003).
-
Alexandre, H., Rousseaux, I. & Charpentier, C. Ethanol adaptation mechanisms in Saccharomyces cerevisiae. Biotechnol. Appl. Biochem. 20, 173–183 (1994).
-
Ochando, T., Mouret, J.-R., Humbert-Goffard, A., Sablayrolles, J.-M. & Farines, V. Impact of initial lipid content and oxygen supply on alcoholic fermentation in champagne-like musts. Food Res. Int. 98, 87–94 (2017).
-
Houtman, A. C. & Plessis, C. S. du. Nutritional deficiencies of clarified white grape juices and their correction in relation to fermentation. South Afr. J. Enol. Vitic. 7, 39–46 (1986).
-
Casalta, E., Salmon, J.-M., Picou, C. & Sablayrolles, J.-M. Grape solids: Lipid composition and role during alcoholic fermentation under enological conditions. Am. J. Enol. Vitic. 70, 147–154 (2019).
-
Evers, M. S. et al. Exploring the unexplored: A characterization of vitamins and vitamers in white grape musts by high-performance liquid chromatography. Food Chem. 398, 133860 (2023).
-
Duncan, J. D., Setati, M. E. & Divol, B. Nicotinic acid availability impacts redox cofactor metabolism in Saccharomyces cerevisiae during alcoholic fermentation. FEMS Yeast Res. 24, foae015 (2024).
-
Saerens, S. M. G. et al. Parameters affecting ethyl ester production by Saccharomyces cerevisiae during fermentation. Appl. Environ. Microbiol 74, 454–461 (2008).
-
Bardi, L., Crivelli, C. & Marzona, M. Esterase activity and release of ethyl esters of medium-chain fatty acids by Saccharomyces cerevisiae during anaerobic growth. Can. J. Microbiol. 44, 1171–1176 (1998).
-
Arneborg, N., Høy, C.-E. & Jørgensen, O. B. The effect of ethanol and specific growth rate on the lipid content and composition of Saccharomyces cerevisiae grown anaerobically in a chemostat. Yeast 11, 953–959 (1995).
-
Beaven, M. J., Charpentier, C. & Rose, A. H. Production and Tolerance of Ethanol in Relation to Phospholipid Fatty-acyl Composition in Saccharomyces cerevisiae NCYC 431. Microbiology 128, 1447–1455 (1982).
-
Jones, R. P. & Greenfield, P. F. Ethanol and the fluidity of the yeast plasma membrane. Yeast 3, 223–232 (1987).
-
Bardi, L., Cocito, C. & Marzona, M. Saccharomyces cerevisiae cell fatty acid composition and release during fermentation without aeration and in absence of exogenous lipids. Int. J. Food Microbiol. 47, 133–140 (1999).
-
Geneix, C., Lafon-Lafourcade, S. & Ribéreau-Gayon, P. Les causes, la prévention et le traitement des arrêts de la fermentation alcoolique. OENO One 17, 205–217 (1983).
-
Lafon-Lafourcade, S., Geneix, C. & Ribéreau-Gayon, P. Inhibition of alcoholic fermentation of grape must by fatty acids produced by yeasts and their elimination by yeast ghosts. Appl. Environ. Microbiol. 47, 1246–1249 (1984).
-
Chassagne, D., Guilloux-Benatier, M., Alexandre, H. & Voilley, A. Sorption of wine volatile phenols by yeast lees. Food Chem. 91, 39–44 (2005).
-
Ángeles Pozo-Bayón, M., Andújar-Ortiz, I. & Moreno-Arribas, M. V. Scientific evidences beyond the application of inactive dry yeast preparations in winemaking. Food Res. Int. 42, 754–761 (2009).
-
Lavigne-Cruège, V. & Dubourdieu, D. Demonstraction and interpretation of the yeast lee ability to adsorb certain volatile thiols contained in wine. OENO One 30, 201–206 (1996).
-
Palacios, S., Vasserot, Y. & Maujean, A. Evidence For Sulfur Volatile Products Adsorption by Yeast Lees. Am. J. Enol. Vitic. 48, 525–526 (1997).
-
Pradelles, R., Vichi, S., Alexandre, H. & Chassagne, D. Influence of the drying processes of yeasts on their volatile phenol sorption capacity in model wine. Int. J. Food Microbiol. 135, 152–157 (2009).
-
Pradelles, R., Alexandre, H., Ortiz-Julien, A. & Chassagne, D. Effects of Yeast Cell-Wall Characteristics on 4-Ethylphenol Sorption Capacity in Model Wine. J. Agric. Food Chem. 56, 11854–11861 (2008).
-
Vasserot, Y., Steinmetz, V. & Jeandet, P. Study of thiol consumption by yeast lees. Antonie Van. Leeuwenhoek 83, 201–207 (2003).
-
Kordialik-Bogacka, E. Surface properties of yeast cells during heavy metal biosorption. Open Chem. 9, 348–351 (2011).
-
Savastru, E., Bulgariu, D., Zamfir, C.-I. & Bulgariu, L. Application of Saccharomyces cerevisiae in the Biosorption of Co(II), Zn(II) and Cu(II) Ions from Aqueous Media. Water 14, 976 (2022).
-
Wang, J. & Chen, C. Biosorption of heavy metals by Saccharomyces cerevisiae: A review. Biotechnol. Adv. 24, 427–451 (2006).
-
Larue, F., Geneix, C., Lafon-Lafourcade, S., Bertrand, A. & Ribéreau-Gayon, P. Premières observations sur le mode d’action des écorces de levure. OENO One 18, 155 (1984).
-
Shinohara, T. Gas Chromatographic Analysis of Volatile Fatty Acids in Wines. Agric. Biol. Chem. 49, 2211–2212 (1985).
-
Moonjai, N. et al. Uptake of Linoleic Acid by Cropped Brewer’s Yeast and its Incorporation in Cellular Lipid Fractions. J. Am. Soc. Brew. Chem. 61, 161–168 (2003).
-
Tesnière, C., Pradal, M. & Legras, J.-L. Sterol uptake analysis in Saccharomyces and non- Saccharomyces wine yeast species. FEMS Yeast Res. 21, foab020 (2021).
-
Kokotou, M. G. Fatty Acid Profiling in Greek Wines by Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS). Separations 11, 321 (2024).
-
Phan, Q., Hoffman, S. & Tomasino, E. Contribution of Lipids to Taste and Mouthfeel Perception in a Model Wine Solution. ACS Food Sci. Technol. 1, 1561–1566 (2021).
-
Restrepo, S., Espinoza, L., Ceballos, A. & Urtubia, A. Production of Fatty Acids during Alcoholic Wine Fermentation under Selected Temperature and Aeration Conditions. Am. J. Enol. Vitic. 70, 169–176 (2019).
-
Yunoki, K. et al. Fatty Acid Compositions of Commercial Red Wines. Biosci. Biotechnol. Biochem. 68, 2623–2626 (2004).
-
Sherman, E. et al. Total Lipids and Fatty Acids in Major New Zealand Grape Varieties during Ripening, Prolonged Pomace Contacts and Ethanolic Extractions Mimicking Fermentation. Fermentation 9, 357 (2023).
-
Jordá, T. & Puig, S. Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae. Genes 11, 795 (2020).
-
Stukey, J. E., McDonough, V. M. & Martin, C. E. The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene. J. Biol. Chem. 265, 20144–20149 (1990).
-
Watanabe, K., Oura, T., Sakai, H. & Kajiwara, S. Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function. Biosci. Biotechnol. Biochem. 68, 721–727 (2004).
-
Choudhary, V., Ojha, N., Golden, A. & Prinz, W. A. A conserved family of proteins facilitates nascent lipid droplet budding from the ER. J. Cell Biol. 211, 261–271 (2015).
-
Ding, J. et al. Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae. Appl. Microbiol Biotechnol. 85, 253–263 (2009).
-
van Roermund, C. W. T., Waterham, H. R., Ijlst, L. & Wanders, R. J. A. Fatty acid metabolism in Saccharomyces cerevisiae. CMLS Cell. Mol. Life Sci. 60, 1838–1851 (2003).
-
Henneberry, A. L. & Sturley, S. L. Sterol homeostasis in the budding yeast, Saccharomyces cerevisiae. Semin. Cell Dev. Biol. 16, 155–161 (2005).
-
Rosenfeld, E., Beauvoit, B., Blondin, B. & Salmon, J.-M. Oxygen consumption by anaerobic Saccharomyces cerevisiae under enological conditions: effect on fermentation kinetics. Appl. Environ. Microbiol. 69, 113–121 (2003).
-
Ness, F. et al. SUT1 is a putative Zn[II]2Cys6-transcription factor whose upregulation enhances both sterol uptake and synthesis in aerobically growing Saccharomyces cerevisiae cells. Eur. J. Biochem. 268, 1585–1595 (2001).
-
Li, Y. et al. Fermentation modeling and machine learning for flavor prediction in low-sodium radish paocai with potassium chloride substitution. npj Sci. Food 9, 156 (2025).
-
Ismail, A. M. et al. Artificial Bee Colony algorithm in estimating kinetic parameters for yeast fermentation pathway. J Integr Bioinform 20, 20220051 (2023).
-
Lai, G. et al. Machine learning methods for predicting the key metabolic parameters of Halomonas elongata DSM 2581 T. Appl. Microbiol Biotechnol. 107, 5351–5365 (2023).
-
Wang, Z.-Z. et al. Fermentation design and process optimization strategy based on machine learning. BioDesign Res. 7, 100002 (2025).
-
Seguinot, P., Ortiz-Julien, A. & Camarasa, C. Impact of Nutrient Availability on the Fermentation and Production of Aroma Compounds Under Sequential Inoculation With M. pulcherrima and S. cerevisiae. Front Microbiol. 11, 305 (2020).
-
Bligh, E. G. & Dyer, W. J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959).
-
Dumont, A. et al. Docosahexaenoic acid inhibits both NLRP3 inflammasome assembly and JNK-mediated mature IL-1β secretion in 5-fluorouracil-treated MDSC: implication in cancer treatment. Cell Death Dis. 10, 1–15 (2019).
-
Thomas, C. et al. LPCAT3 deficiency in hematopoietic cells alters cholesterol and phospholipid homeostasis and promotes atherosclerosis. Atherosclerosis 275, 409–418 (2018).
-
Nguyen, M. et al. High plasma concentration of non-esterified polyunsaturated fatty acids is a specific feature of severe COVID-19 pneumonia. Sci. Rep. 11, 10824 (2021).
-
Mouillot, T. et al. Fatty acid composition of the erythrocyte membrane and risk of hepatocellular carcinoma in cirrhotic patients. Aliment. Pharmacol. Ther. 52, 1503–1515 (2020).
-
Scott, R. P. W. Principles and Practice of Chromatography (Library for Science, 2003)
-
Vial, G. et al. Imeglimin normalizes glucose tolerance and insulin sensitivity and improves mitochondrial function in liver of a high-fat, high-sucrose diet mice model. Diabetes 64, 2254–2264 (2014).
-
Ménégaut, L. et al. Specific enrichment of 2-arachidonoyl-lysophosphatidylcholine in carotid atheroma plaque from type 2 diabetic patients. Atherosclerosis 251, 339–347 (2016).
-
Denimal, D. et al. Plasma 16:0 ceramide as a marker of cardiovascular risk estimated by carotid intima-media thickness in people with type 2 diabetes. Diab. Metab. 50, 101542 (2024).
-
Cajka, T. & Fiehn, O. LC/MS Method for Comprehensive Analysis of Plasma Lipids. Agilent Technologies Application Note 5991-9280EN (2018)
-
Wickham, H. et al. ggplot2: Create Elegant Data Visualisations Using the Grammar of Graphics. R package version 3.5.1 https://CRAN.R-project.org/package=ggplot2 (2024).
-
Hollander, M., Wolfe, D. A. & Chicken, E. Nonparametric Statistical Methods. (John Wiley & Sons, 2013).
-
Husson, F., Josse, J., Le, S. & Mazet, J. FactoMineR: Multivariate Exploratory Data Analysis and Data Mining. R package version 2.11 https://CRAN.R-project.org/package=FactoMineR (2024).
