Efficacy of thyme oil and nano-formulated derivatives against Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)

efficacy-of-thyme-oil-and-nano-formulated-derivatives-against-rhipicephalus-sanguineus-sensu-lato-(acari:-ixodidae)
Efficacy of thyme oil and nano-formulated derivatives against Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)

References

  1. Henrioud, A. N. Towards sustainable parasite control practices in livestock production with emphasis in Latin America. Vet. Parasitol. 180 (1–2), 2–11 (2011).

    Google Scholar 

  2. Sousa, R. & Bacellar, F. Morbi-mortalidade Por Rickettsia Conorii Em Portugal. Rev Bras Parasitol Vet. 13, 180–184 (2004).

    Google Scholar 

  3. Matsumoto, K., Brouqui, P., Raoult, D. & Parola, P. Experimental infection models of ticks of the Rhipicephalus sanguineus group with Rickettsia Conorii. Vector Borne Zoon Dis. 5 (4), 363–372 (2005).

    Google Scholar 

  4. Demma, L. J. et al. Rocky mountain spotted fever from an unexpected tick vector in Arizona. N Engl. J. Med. 353 (6), 587–594 (2005).

    Google Scholar 

  5. Hegab, A. A. et al. Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt. Parasit. Vectors. 15 (1), 222 (2022).

    Google Scholar 

  6. Hegab, A. A. et al. Occurrence and genotyping of Theileria equi in dogs and associated ticks in Egypt. Med. Vet. Entomol. 37 (2), 252–262 (2023).

    Google Scholar 

  7. Eiden, A. L., Kaufman, P. E., Oi, F. M., Allan, S. A. & Miller, R. J. Detection of permethrin resistance and fipronil tolerance in Rhipicephalus sanguineus (Acari: Ixodidae) in the united States. J. Med. Entomol. 52 (3), 429–436 (2015).

    Google Scholar 

  8. Rodriguez-Vivas, R. I., Ojeda-Chi, M. M. & Trinidad-Martinez, I. & De León, A. P. First documentation of ivermectin resistance in Rhipicephalus sanguineus sensu lato (Acari: Ixodidae). Vet. Parasitol 233, 9–13 (2017).

  9. Rodriguez-Vivas, R. I., Ojeda‐Chi, M. M., Trinidad‐Martinez, I. & Bolio‐González, M. E. First report of Amitraz and Cypermethrin resistance in Rhipicephalus sanguineus sensu Lato infesting dogs in M Exico. Med. Vet. Entomol. 31 (1), 72–77 (2017).

    Google Scholar 

  10. Becker, S. et al. Resistance to deltamethrin, fipronil and Ivermectin in the brown dog tick, Rhipicephalus sanguineus sensu stricto, Latreille (Acari: Ixodidae). Tick. Borne Dis. 10 (5), 1046–1050 (2019).

    Google Scholar 

  11. Borges, L. M. F., Ferri, P. H., Silva, W. J., Silva, W. C. & Silva, J. G. In vitro efficacy of extracts of Melia Azedarach against the tick Boophilus Microplus. Med. Vet. Entomol. 17 (2), 228–231 (2003).

    Google Scholar 

  12. Abdel-Shafy, S. & Soliman, M. M. M. Toxicity of some essential oils on eggs, larvae and females of Boophilus annulatus (Acari: ixodida: Amblyommidae) infesting cattle in Egypt. Acarologia 44 (1–2), 23–30 (2004).

    Google Scholar 

  13. Dietrich, G. et al. Repellent activity of fractioned compounds from Chamaecyparis nootkatensis essential oil against nymphal Ixodes scapularis (Acari: Ixodidae). Med. Vet. Entomol. 43 (5), 957–961 (2006).

    Google Scholar 

  14. Ribeiro, V. L. S., Toigo, E., Bordignon, S. A., Gonçalves, K. & von Poser, G. Acaricidal properties of extracts from the aerial parts of Hypericum polyanthemum on the cattle tick Boophilus Microplus. Vet. Parasitol. 147 (1–2), 199–203 (2007).

    Google Scholar 

  15. Facey, P. C., Porter, R. B., Reese, P. B. & Williams, L. A. Biological activity and chemical composition of the essential oil from Jamaican Hyptis verticillata Jacq. J. Agric. Food Chem. 53 (12), 4774–4777 (2005).

    Google Scholar 

  16. Panella, N. A. et al. Use of novel compounds for pest control: insecticidal and acaricidal activity of essential oil components from Heartwood of Alaska yellow Cedar. Med. Vet. Entomol. 42 (3), 352–358 (2005).

    Google Scholar 

  17. Tunón, H., Thorsell, W., Mikiver, A. & Malander, I. Arthropod repellency, especially tick (Ixodes ricinus), exerted by extract from Artemisia abrotanum and essential oil from flowers of Dianthus Caryophyllum. Fitoterapia 77 (4), 257–261 (2006).

    Google Scholar 

  18. Bakkali, F., Averbeck, S., Averbeck, D. & Idaomar, M. Biological effects of essential oils–a review. Food Chem. Toxicol. 46 (2), 446–475 (2008).

    Google Scholar 

  19. Wang, L., Li, X., Zhang, G., Dong, J. & Eastoe, J. Oil-in-water nanoemulsions for pesticide formulations. J. Colloid Interface Sci. 314 (1), 230–235 (2007).

    Google Scholar 

  20. Halat, D. H., Krayem, M., Khaled, S. & Younes, S. A focused insight into thyme: Biological, Chemical, and therapeutic properties of an Indigenous mediterranean herb. Nutrients 14 (10) (2022).

  21. Galovičová, L. et al. Thymus vulgaris essential oil and its biological activity. Plants 10 (9) (2021).

  22. Chahboun, N. et al. Chemical composition, biological activities, and anti-corrosion performance of Moroccan essential oil Thymus vulgaris from the Oued amlil region, Taza. Int. J. Electro chem. Sci. 19 (12), 100859 (2024).

    Google Scholar 

  23. Pavela, R. & Sedlák, P. Post-application temperature as a factor influencing the insecticidal activity of essential oil from Thymus vulgaris. Ind. Crop Prod. 113, 46–49 (2018).

    Google Scholar 

  24. Alibeigi, Z., Rakhshandehroo, E., Saharkhiz, M. J. & Alavi, A. M. The acaricidal and repellent activity of the essential and nano essential oil of Thymus vulgaris against the larval and engorged adult stages of the brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae). BMC Vet. Res. 21, 135 (2025).

    Google Scholar 

  25. Tabari, M. A., Youssefi, M. R., Maggi, F. & Benelli, G. Toxic and repellent activity of selected monoterpenoids (thymol, carvacrol and linalool) against the castor bean tick, Ixodes ricinus (Acari: Ixodidae). Vet. Parasitol. 245, 86–91 (2017).

    Google Scholar 

  26. Coelho, L. et al. Combination of thymol and Eugenol for the control of Rhipicephalus sanguineus sensu lato: evaluation of synergism on immature stages and formulation development. Vet. Parasitol. 277, 108989 (2020).

    Google Scholar 

  27. Matos, R. S. et al. Determination of the susceptibility of unengorged larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae) to different methods of dissolving thymol. Parasitol. Res. 108, 1541–1549 (2014).

  28. Costa-Júnior, L. M., Miller, R. J., Alves, P. B., Blank, A. F. & Li, A. Y. León, A. A. P. Acaricidal efficacies of Lippia gracilis essential oil and its phytochemicals against organophosphate-resistant and susceptible strains of Rhipicephalus (Boophilus) Microplus. Vet. Parasitol. 228, 60–64 (2016). de.

    Google Scholar 

  29. Arafa, W. M., Aboelhadid, S. M., Moawad, A., Shokeir, K. M. & Ahmed, O. Toxicity, repellency and anti-cholinesterase activities of thymol-eucalyptus combinations against phenotypically resistant Rhipicephalus annulatus ticks. Exp. Appl. Acarol. 81, 265–277 (2020).

    Google Scholar 

  30. Campolo, O., Giunti, G., Laigle, M., Michel, T. & Palmeri, V. Essential oil-based nano-emulsions: effect of different surfactants, sonication and plant species on physicochemical characteristics. Ind. Crop Prod. 157, 112935 (2020).

    Google Scholar 

  31. Modafferi, A. et al. Bioactivity of Allium sativum essential oil-based nano-emulsion against Planococcus citri and its predator Cryptolaemus Montrouzieri. Ind. Crop Prod. 208, 117837 (2024).

    Google Scholar 

  32. Nogueira, J. A. et al. Repellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus Microplus larvae. Exp. Parasitol. 215, 107919 (2020).

    Google Scholar 

  33. Dos Santos, D. S. et al. Nanostructured cinnamon oil has the potential to control Rhipicephalus Microplus ticks on cattle. Exp. Appl. Acarol. 73, 129–138 (2017).

    Google Scholar 

  34. Baldissera, M. D., Stefani, L. M. & Da Silva, A. S. Effects of essential oil of Eucalyptus globulus loaded in nanoemulsions and in nanocapsules on reproduction of cattle tick (Rhipicephalus microplus). Arc De Zootec. 67, 494–498 (2018).

    Google Scholar 

  35. Marimuthu, S. et al. Evaluation of green synthesized silver nanoparticles against parasites. Parasitol. Res. 108, 1541–1549 (2011).

    Google Scholar 

  36. Nabil, M. et al. Acaricidal efficacy of silver nanoformulations of Commiphora molmol and Zingiber officinale against the camel Tick, Hyalomma dromedarii (Ixodida: Ixodidae). Inorg. Chem. Commun. 147, 110229 (2023).

    Google Scholar 

  37. Majeed, Q. A. H. et al. Acaricidal, larvacidal, and repellent activity of green synthesized silver nanoparticles against Hyalomma dromedarii. Trop. Biomed. 40 (3), 356–362 (2023).

    Google Scholar 

  38. Kumar, B., Smita, K., Cumbal, L. & Debut, A. Green synthesis of silver nanoparticles using Andrographis paniculata and their acaricidal activity against Rhipicephalus (Boophilus) Microplus. Vet. Parasitol. 225, 47–53 (2016).

    Google Scholar 

  39. Abo Talep, E., Abuowarda, M., Abdel-Shafy, S., Mahmoud, N. E. & Fahmy, M. Seasonal variation and morphometric differentiation of Egyptian strain of Rhipicephalus sanguineus (Acari: Ixodidae). Egypt. J. Vet. Sci. 55 (4), 1109–1118 (2024).

    Google Scholar 

  40. Abdel-Ghany, H. S. et al. In vitro acaricidal activity of green synthesized nickel oxide nanoparticles against the camel tick, Hyalomma dromedarii (Ixodidae), and its toxicity on Swiss albino mice. Exp. Appl. Acarol. 83, 611–633 (2021).

    Google Scholar 

  41. Abdel-Ghany, H. S. M. et al. Acaricidal activity of some medicinal plant extracts against different developmental stages of the camel tick Hyalomma dromedarii. Adv. Anim. Vet. Sci. 9 (5), 722–733 (2021).

    Google Scholar 

  42. Sugumar, S. et al. Nanoemulsion of Eucalyptus oil and its larvicidal activity against Culex quinquefasciatus. Bull. Entomol. Res. 104 (3), 393–402 (2014).

    Google Scholar 

  43. Abdel-Ghany, H. S. et al. Acaricidal efficacy of biosynthesized zinc oxide nanoparticles against Hyalomma dromedarii (Acari: Ixodidae) and their toxic effects on Swiss albino mice. Acta Parasitol. 67 (2), 878–891 (2022).

    Google Scholar 

  44. Brody, A. R. & Wharton, G. W. Use of glycerol-KCl in scanning microscopy of Acari. Entomol. Soc. Am. Ann. (1971).

  45. Homsher, P. J. & Sonenshine, D. E. Scanning electron microscopy of ticks for systematic studies 2. Structure of Hallerʼs organ in Ixodes brunneus and Ixodes frontalis. J. Med. Entomol. 14 (1), 93–97 (1977).

    Google Scholar 

  46. Keirans, J. E., Clifford, C. M. & Corwin, D. Ixodes sigelos, n. sp.(Acarina: Ixodidae), a parasite of rodents in Chile, with a method for Preparing ticks for examination by scanning electron microscopy. Acarol 18 (2), 217–225 (1976).

    Google Scholar 

  47. Alves, F. M. et al. Heat-stressed Metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick Rhipicephalus sanguineus. Parasitol. Res. 116, 111–121 (2017).

    Google Scholar 

  48. Abuowarda, M. M., Haleem, M. A., Elsayed, M., Farag, H. & Magdy, S. Bio-pesticide control of the brown dog tick (Rhipicephalus sanguineus) in Egypt by using two entomopathogenic fungi (Beauveria Bassiana and Metarhizium anisopliae). Int. J. Vet. Sci. 9 (2), 175–181 (2020).

    Google Scholar 

  49. Finney, D. J. A statistical treatment of the sigmoid response curve. Probit Analysis. 633 (Cambridge University Press, 1971).

    Google Scholar 

  50. Irache, J. M., Esparza, I., Gamazo, C., Agüeros, M. & Espuelas, S. Nanomedicine: novel approaches in human and veterinary therapeutics. Vet. Parasitol. 180 (1–2), 47–71 (2011).

    Google Scholar 

  51. de Oliveira, A. A., França, L. P., Ramos, A. D. S., Ferreira, J. L. P., Maria, A.C. B., Oliveira, K. M., de Andrade Silva, J. R. Larvicidal, adulticidal and repellent activities against Aedes aegypti L. of two commonly used spices, Origanum vulgare L. and Thymus vulgaris L. S. Afr. J. Bot. 140, 17–24. (2021).

  52. Bishr, M. M. & Salama, O. M. Inter and intra GC-MS differential analysis of the essential oils of three Mentha species growing in Egypt. Futur J. Pharm. Sci. 4, 53–56 (2018).

    Google Scholar 

  53. Figueiredo, A. C., Barroso, J. G., Pedro, L. G. & Scheffer, J. J. C. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour. Fragr. J. 23, 213–226 (2008).

    Google Scholar 

  54. Massoud, M. A., Adel, M. M., Zaghloul, O. A., Mohamed, M. I. E. & Abdel-Rheim, K. H. Eco-friendly nano-emulsion formulation of Mentha Piperita against stored product pest Sitophilus oryzae. Adv. Crop Sci. Technol. 6, 404 (2018).

    Google Scholar 

  55. Nenaah, G. E., Almadiy, A. A., Al-Assiuty, B. A. & Mahnashi, M. H. The essential oil of Schinus terebinthifolius and its nanoemulsion and isolated monoterpenes: investigation of their activity against Culex pipiens with insights into the adverse effects on non-target organisms. Pest Manag Sci. 78 (3), 1035–1047. https://doi.org/10.1002/ps.6715 (2022).

    Google Scholar 

  56. Athanassiou, C. G. et al. Nanoparticles for pest control: current status and future perspectives. J. Pest Sci. 91 (1), 1–15. https://doi.org/10.1007/s10340-017-0898-0 (2018).

    Google Scholar 

  57. Lobato Rodrigues, A. B. et al. M. D. S. Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae. PloS One. 16 (7), e0254225. https://doi.org/10.1371/journal.pone.0254225 (2021).

  58. Novato, T. P. et al. Acaricidal activity of carvacrol and thymol on acaricide-resistant Rhipicephalus Microplus (Acari: Ixodidae) populations and combination with cypermethrin: is there cross-resistance and synergism? Vet. Parasitol. 310, 109787. https://doi.org/10.1016/j.vetpar.2022.109787 (2022).

    Google Scholar 

  59. Wu, L. et al. Acaricidal activity and synergistic effect of thyme oil constituents against Carmine spider mite (Tetranychus cinnabarinus (Boisduval)). Mol 22 (11), 1873. https://doi.org/10.3390/molecules22111873 (2017).

    Google Scholar 

  60. Parvin, N., Aslam, M., Joo, S. W. & Mandal, T. K. Nano-Phytomedicine: Harnessing Plant-Derived phytochemicals in nanocarriers for targeted human health applications. Mol 30 (15), 3177. https://doi.org/10.3390/molecules30153177 (2025).

    Google Scholar 

  61. da Silva, L. C. et al. In vitro acaricidal activity of cymbopogon citratus, cymbopogon nardus and mentha arvensis against R. microplus (Acari: Ixodidae). Exp. Parasitol. 216, 107937. https://doi.org/10.1016/j.exppara.2020.107937 (2020).

    Google Scholar 

  62. Agwunobi, D. O., Pei, T., Wang, K., Yu, Z. & Liu, J. Effects of the essential oil from Cymbopogon citratus on mortality and morphology of the tick Haemaphysalis longicornis (Acari: Ixodidae). Exp. Appl. Acarol. 81 (1), 37–50 (2020).

    Google Scholar 

  63. Dey, S., Sinha, B. & Kalita, J. Effect of Eupatorium adenophorum spreng leaf extracts on the mustard aphid, Lipaphis erysimi kalt: A scanning electron microscope study. Microsc Res. Tech. 66, 31–36 (2005).

    Google Scholar 

  64. Ellse, L. & Wall, R. The use of essential oils in veterinary ectoparasite control: A review. Med. Vet. Entomol. 28, 233–243 (2014).

    Google Scholar 

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