First comparison of commercial systems to prepare nanofat: technical performances and biological quality differ among obtained products

AEVs:

Adipocyte-derived extracellular vesicles

ASC:

Adipose-derived stem/stromal cells

ASC-EVs:

Adipose stromal/stem cell-derived extracellular vesicles

AT:

Adipose tissue

ATMP:

Advanced therapy medicinal product

CFU-F:

Colony forming units-fibroblasts

CM:

Conditioned media

DAPI:

4′,6-Diamidino-2-phenylindole (utilisé pour marquage nucléaire)

EBM:

Endothelial basal medium

ECM:

Extracellular matrix

EEVs:

Endothelial-derived extracellular vesicles

ErEVs:

Erythrocyte-derived extracellular vesicles

EVs:

Extracellular vesicles

FBS:

Fetal bovine serum

FMO:

Fluorescence minus one

HSA:

Human serum albumin

IFATS:

International federation for adipose therapeutics and science

IGF:

Insulin-like growth factor

IQR:

Interquartile range

ISCT:

International society for cellular therapy

LEVs:

Leukocyte-derived extracellular vesicles

MSC-EVs:

Mesenchymal stromal/stem cell-derived EVs

ORO:

Oil Red O

PBS:

Phosphate-buffered saline

PDGF:

Platelet-derived growth factor

PEVs:

Platelet-derived extracellular vesicles

PFA:

Paraformaldehyde

SVF:

Stromal vascular fraction

VEGF:

Vascular endothelial growth factor

VNC:

Viable nucleated cells

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Author notes

1. These authors contributed equally: Mélanie Velier and Jérémy Magalon.

Authors and Affiliations

1. Internal Medicine and Therapeutics Department, CHU La Timone, AP-HM, Marseille, France

   Robin Arcani & Aurélie Daumas

2. Aix-Marseille University, INSERM, INRAE, C2VN, Marseille, France

   Robin Arcani, Maxime Abellan, Stéphanie Simoncini, Stéphane Robert, Anouck Zavarro, Romaric Lacroix, Françoise Dignat George, Florence Sabatier, Aurélie Daumas, Mélanie Velier & Jérémy Magalon

3. Plastic Surgery Department, CHU Conception, AP-HM, Marseille, France

   Maxime Abellan

4. ExAdEx-Innov, 28 Avenue de Valombrose, Nice, France

   Vincent Dani

5. Laboratoire de Culture et Thérapie Cellulaire, Cell Therapy Department, CHU de La Conception, INSERM CIC BT 1409, 147 Boulevard Baille, AP-HM, 13005, Marseille, France

   Cécilia Bec, Florence Sabatier, Mélanie Velier & Jérémy Magalon

6. Clinical Pharmacology and Drug Surveillance, Marseille University Hospital, Marseille, France

   Elisabeth Jouve

7. Department of Hematology, Biogenopole, CHU La Timone, APHM, Marseille, France

   Laurent Arnaud, Romaric Lacroix & Françoise Dignat George

8. Centre for Aesthetic & Regenerative Medicine, Clinique Genolier, Genolier, Switzerland

   Sophie Menkes

9. Remedex, Regenerative Medicine Department of Excellence, Marseille, France

   Guy Magalon

Contributions

RA, MA, SS, VD, SR, AZ, LA, CB, SM and MV performed experiments and collected data. RA, SS, VD, SR, EJ, LA, RL, MV and JM analysed and interpreted the results. GM, AD, MV, and JM conceived the study, supervised the project, and contributed to data interpretation. GM, RL, FDG, FS, and AD provided critical input on study design, data interpretation, and manuscript revision. RA, MA, SS, SR, LA, VD, MV and JM drafted the manuscript. All the authors have read and approved the final version of the manuscript.

Corresponding author

Correspondence to Jérémy Magalon.

Competing interests

GM, FS and JM are cofounders of the Remedex Network. JM received honoraria for educational support from Fidia Pharmaceuticals, Horiba, Arthrex, Horus Pharma and Macopharma. These manufacturers had no role in the development of this study or its decision for publication. The authors declare that they have no competing interests.

Ethics approval and consent to participate

All donors who participated in the study received an information and nonopposition notice and did not express any objection to the use of their adipose tissue.

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