In vivo chemogenetic RNA editing of macrophages by bioengineered viruses for sepsis treatment

in-vivo-chemogenetic-rna-editing-of-macrophages-by-bioengineered-viruses-for-sepsis-treatment
In vivo chemogenetic RNA editing of macrophages by bioengineered viruses for sepsis treatment

Data availability

All data supporting the findings of this study are available within the Article and its Supplementary Information. Source data are provided with this paper. The raw data of sequencing have been deposited at the NCBI Gene Expression Omnibus under accession number: PRJNA1328038 & PRJNA1347866Source data are provided with this paper.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (82425055 to Y.P. and 82504685 to X.Y.), the Natural Science Foundation of Zhejiang Province (LMS25H300002 to X.Y.), Key R&D Program of Zhejiang (2024SSYS0028 to Y.P.), the Postdoctoral Fellowship Program of CPSF (GZB20240672 to X.Y.), and the China Postdoctoral Science Foundation (2024M762890 and 2025T180980 to X.Y.). We thank Y. Chen, F. Zhang and B. Li for help in this project. We also thank J. Pan and D. Wu of the Research and Service Center (College of Pharmaceutical Sciences, Zhejiang University) for technical assistance with qPCR analysis and confocal microscopy imaging.

Author information

Author notes

  1. These authors contributed equally: Wensong Xi, Youcui Xu.

Authors and Affiliations

  1. State Key Laboratory of Macromolecular Drugs and Large-scale Preparation, Hefei Center; School of Pharmacy, Anhui Medical University, Hefei, China

    Wensong Xi, Youcui Xu & Yuan Ping

  2. Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, China

    Wensong Xi, Xiaojie Yan & Yuan Ping

  3. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

    Wensong Xi, Weiwei Bao, Zhixing Ding, Hu Xiao, Jiamin Yang, Xiaojie Yan & Yuan Ping

  4. Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, Research and Industrialization of New Drug Release Technology Joint Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei, China

    Youcui Xu

Authors

  1. Wensong Xi
  2. Youcui Xu
  3. Weiwei Bao
  4. Zhixing Ding
  5. Hu Xiao
  6. Jiamin Yang
  7. Xiaojie Yan
  8. Yuan Ping

Contributions

X.Y. and Y.P. conceived the project and designed experiments. W.X., Y.X., X.Y., W.B., Z.D., H.X., and J.Y. performed the experiments. X.Y. and H.X. constructed and verified the plasmids. W.X. and Y.X., X.Y. analyzed the data and wrote the manuscript. X.Y. and Y.P. supervised the project and wrote the manuscript.

Corresponding authors

Correspondence to Xiaojie Yan or Yuan Ping.

Ethics declarations

Competing interests

The Authors declare the following competing interests: A patent application related to the biomineralized lentivirus platform described in this work has been filed with the China National Intellectual Property Administration by Zhejiang University, with W.X. and Y.P. as inventors (application number CN202211558965.3). The remaining authors declare no competing interests.

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Nature Communications thanks Yilai Shu and Jun Wang for their contribution to the peer review of this work. A peer review file is available.

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Xi, W., Xu, Y., Bao, W. et al. In vivo chemogenetic RNA editing of macrophages by bioengineered viruses for sepsis treatment. Nat Commun (2025). https://doi.org/10.1038/s41467-025-67655-y

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