Joint profiling of chromatin accessibility and CRISPR edits via double-stranded DNA deaminases

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Joint profiling of chromatin accessibility and CRISPR edits via double-stranded DNA deaminases
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Nature Methods (2025)Cite this article

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Targeted deaminase-accessible chromatin sequencing (TDAC-seq) measures chromatin accessibility across long chromatin fibers at targeted loci using double-stranded DNA cytidine deaminases. When combined with pooled CRISPR mutational screening, TDAC-seq enables the high-throughput detection of changes in chromatin accessibility following CRISPR perturbations, allowing fine mapping of sequence–function relationships within endogenous cis-regulatory elements.

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Fig. 1: TDAC-seq coupled with pooled CRISPR-mutational scanning.

References

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This is a summary of: Roh, H. et al. Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase. Nat. Methods https://doi.org/10.1038/s41592-025-02811-2 (2025).

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Joint profiling of chromatin accessibility and CRISPR edits via double-stranded DNA deaminases. Nat Methods (2025). https://doi.org/10.1038/s41592-025-02812-1

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  • DOI: https://doi.org/10.1038/s41592-025-02812-1