Data availability
All data supporting the findings of this study are available within the paper or upon reasonable request from the corresponding author (S.K.N.).
References
-
Altamirano, C., Paredes, C., Cairó, J. J. & Gòdia, F. Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine. Biotechnol. Prog. 16, 69–75 (2000).
-
Leong, D. S. Z. et al. Evaluation and use of disaccharides as energy source in protein-free mammalian cell cultures. Sci. Rep. 7, 45216 (2017).
-
Leong, D. S. Z. et al. Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody. Sci. Rep. 8, 4037 (2018).
-
Chiba, S. Molecular mechanism in alpha-glucosidase and glucoamylase. Biosci. Biotechnol. Biochem. 61, 1233–1239 (1997).
-
Quezada-Calvillo, R., Simsek, M., Juarez, J. & Nichols, B. Protein synthesis controls the activity of Maltase-Glucoamylase and Sucrase-Isomaltase in non-intestinal tissues. FASEB J. 29(596), 18 (2015).
-
Selvarasu, S. et al. Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture. Biotechnol. Bioeng. 109, 1415–1429 (2012).
-
Xu, S. et al. von Hippel-Lindau protein maintains metabolic balance to regulate the survival of naive B lymphocytes. Iscience. 17, 379–392 (2019).
-
Millard, P., Letisse, F., Sokol, S. & Portais, J.-C. IsoCor: correcting MS data in isotope labeling experiments. Bioinformatics 28, 1294–1296 (2012).
-
Scheffler, O. & Ahearn, G. A. Functional characterization of a novel disaccharide transporter in lobster hepatopancreas. J. Comp. Physiol. B 187, 563–573 (2017).
-
Meyer, H., Vitavska, O. & Wieczorek, H. Identification of an animal sucrose transporter. J. Cell Sci. 124, 1984–1991 (2011).
-
Clissold, S. P. & Edwards, C. Acarbose. Drugs 35, 214–243 (1988).
-
Saul R, Ghidoni J J, Molyneuxt R J, Elbein A D 1985. Castanospermine inhibits a-glucosidase activities and alters glycogen distribution in animals. 5
-
Saul, R., Chambers, J. P., Molyneux, R. J. & Elbein, A. D. Castanospermine, a tetrahydroxylated alkaloid that inhibits beta-glucosidase and beta-glucocerebrosidase. Arch. Biochem. Biophys. 221, 593–597 (1983).
-
Hermans, M. M., Wisselaar, H. A., Kroos, M. A., Oostra, B. A. & Reuser, A. J. Human lysosomal alpha-glucosidase: functional characterization of the glycosylation sites. Biochem. J. 289, 681–686 (1993).
-
Moreland, R. J. et al. Lysosomal acid α-glucosidase consists of four different peptides processed from a single chain precursor. J. Biol. Chem. 280, 6780–6791 (2005).
-
Cooper, G. M. in Cell Mol. Approach 2nd Ed. (Sinauer Associates, 2000). at <https://www.ncbi.nlm.nih.gov/books/NBK9847/>
-
Choa, J. B. D. et al. Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells. Cytotechnology 75, 219–229 (2023).
Acknowledgements
This project was supported by the Bioprocessing Technology Institute and Biomedical Research Council of the Agency for Science, Technology and Research (A*STAR), Singapore. Y.S.H and S.K.N. acknowledge the funding support by A*STAR under its RIE2025 Human Health and Potential (HHP) Industry Alignment Fund Pre-Positioning (IAF-PP) Grant number H25J6a0034.
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Tan, T.R.M., Yip, L.Y., Tan, J.G.L. et al. Maltose metabolism in serum free CHO culture involves lysosomal acid α-glucosidase. Sci Rep (2025). https://doi.org/10.1038/s41598-025-30901-w
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DOI: https://doi.org/10.1038/s41598-025-30901-w
