Modified protein selection strategy based on Escherichia coli’s Hitchhiker transport and validation through selection of nanobodies targeting bovine interferon gamma

modified-protein-selection-strategy-based-on-escherichia-coli’s-hitchhiker-transport-and-validation-through-selection-of-nanobodies-targeting-bovine-interferon-gamma
Modified protein selection strategy based on Escherichia coli’s Hitchhiker transport and validation through selection of nanobodies targeting bovine interferon gamma

Data availability

The datasets supporting the conclusions of this article are included within the article and its supplementary information file.

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Funding

This research project is supported by King Mongkut’s University of Technology Thonburi (KMUTT), Thailand Science Research and Innovation (TSRI), and National Science, Research and Innovation Fund (NSRF) Fiscal year 2025 Grant No. FRB680074/0164 and Agricultural Research Development Agency (ARDA) Grant No. CRP6405031040 to (D.W.-Z. and K.P.), Petchra Pra Jom Klao Ph.D. Research Scholarship from King Mongkut’s University of Technology Thonburi Grant No. 1/2564 (to S.S.).

Author information

Authors and Affiliations

  1. Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, Bangkok, 10140, Thailand

    Suwitchaya Sirimanakul, Apisitt Thaiprayoon, Kwanpet Intasurat, Warisara Kasemsukwimol, Nonth Submunkongtawee & Dujduan Waraho-Zhmayev

  2. Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA, 02115, USA

    Joseph D. Hurley & Andrew C. Kruse

  3. Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, 10800, Thailand

    Lueacha Tabtimmai

  4. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Kittitat Jaengwang & Kiattawee Choowongkomon

  5. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand

    Pinpunya Riangrungroj

  6. National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand

    Jeerapond Leelawattanachai

  7. Department of Livestock Development, National Institute of Animal Health, Bangkok, 10900, Thailand

    Kreeson Packthongsuk

Authors

  1. Suwitchaya Sirimanakul
  2. Joseph D. Hurley
  3. Apisitt Thaiprayoon
  4. Kwanpet Intasurat
  5. Warisara Kasemsukwimol
  6. Nonth Submunkongtawee
  7. Lueacha Tabtimmai
  8. Kittitat Jaengwang
  9. Kiattawee Choowongkomon
  10. Pinpunya Riangrungroj
  11. Jeerapond Leelawattanachai
  12. Andrew C. Kruse
  13. Kreeson Packthongsuk
  14. Dujduan Waraho-Zhmayev

Contributions

Conceptualization: K.P., D.W.-Z.; Methodology: S.S., J.D.H., A.T., K.I., W.K., N.S., P.R., J.L. K.P., D.W.-Z.;Investigation: S.S., J.D.H., A.T., K.P.; Formal analysis: S.S., J.D.H., L.T., K.J., K.P., D.W.-Z.; Software: S.S., J.D.H., L.T., K.J., K.C.; Data curation: S.S., J.D.H., K.J.; Visualization: S.S.; Supervision: D.W.-Z.; Project administration: K.P., D.W.-Z.; Resources: L.T., K.C., P.R., A.C.K., K.P., D.W.-Z.; Funding acquisition: S.S., D.W.-Z., K.P., A.C.K.; Writing—original draft preparation: S.S., J.D.H., A.C.K., D.W.-Z.; Writing—review and editing: S.S., L.T., K.C., P.R., J.L., K.P., D.W.-Z.

Corresponding author

Correspondence to Dujduan Waraho-Zhmayev.

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Competing interests

A.C.K. is a cofounder and consultant for biotechnology companies Tectonic Therapeutic and Seismic Therapeutic, and also for the Institute for Protein Innovation, a nonprofit research institute. The other authors do not have any competing interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Sirimanakul, S., Hurley, J.D., Thaiprayoon, A. et al. Modified protein selection strategy based on Escherichia coli’s Hitchhiker transport and validation through selection of nanobodies targeting bovine interferon gamma. Sci Rep (2026). https://doi.org/10.1038/s41598-026-43280-7

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  • DOI: https://doi.org/10.1038/s41598-026-43280-7

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