Multi enzyme production and alginate encapsulation from Bacillus subtilis for stable feed additives

1. Deckers, M., Deforce, D., Fraiture, M. A. & Roosens, N. H. Genetically modified micro-organisms for industrial food enzyme production: an overview. Foods 9, 326 (2020).

   Google Scholar 

2. Verduzco-Oliva, R. & Gutierrez-Uribe, J. A. Beyond enzyme production: solid state fermentation (SSF) as an alternative approach to produce antioxidant polysaccharides. Sustainability 12, 495 (2020).

   Google Scholar 

3. Wang, P., Peng, C., Xie, X., Deng, X. & Weng, M. Research progress on the fibrinolytic enzymes produced from traditional fermented foods. Food Sci. Nutr. 11, 5675–5688 (2023).

   Google Scholar 

4. Ding, Q. & Ye, C. Microbial cell factories based on filamentous bacteria, yeasts, and fungi. Microb. Cell. Fact. 22, 20 (2023).

   Google Scholar 

5. Patel, A. K., Dong, C. D., Chen, C. W., Pandey, A. & Singhania, R. R. in Biotechnology of microbial enzymes 25–57Elsevier, (2023).

6. Akinsemolu, A. A., Onyeaka, H., Odion, S. & Adebanjo, I. Exploring Bacillus subtilis: Ecology, biotechnological applications, and future prospects. J. Basic Microbiol. 64, 2300614 (2024).

   Google Scholar 

7. Liu, X. & Kokare, C. in Biotechnology of microbial enzymes 405–444 (Elsevier, 2023).

8. Shi, H. et al. Bacillus subtilis field spray on alpine meadows promotes digestibility in Tibetan sheep via increasing the nutrient quality of herbage and enhancing rumen bacterial populations. Anim. Feed Sci. Technol. 310, 115920 (2024).

   Google Scholar 

9. Bontà, V. et al. An in vitro study on the role of cellulases and Xylanases of Bacillus subtilis in dairy cattle nutrition. Microorganisms 12, 300 (2024).

   Google Scholar 

10. Bhattacharya, R., Arora, S. & Ghosh, S. Bioprocess optimization for food-grade cellulolytic enzyme production from sorghum waste in a novel solid-state fermentation bioreactor for enhanced Apple juice clarification. J. Environ. Manage. 358, 120781 (2024).

    Google Scholar 

11. Thirunavukkarasu, M., Sawle, Y. & Lala, H. A comprehensive review on optimization of hybrid renewable energy systems using various optimization techniques. Renew. Sustain. Energy Rev. 176, 113192 (2023).

    Google Scholar 

12. Abdollahzadeh, R., Pazhang, M., Najavand, S., Fallahzadeh-Mamaghani, V. & Amani-Ghadim, A. R. Screening of pectinase-producing bacteria from farmlands and optimization of enzyme production from selected strain by RSM. Folia Microbiol. 65, 705–719 (2020).

    Google Scholar 

13. Pereira, L. M. S., Milan, T. M. & Tapia-Blácido, D. R. Using response surface methodology (RSM) to optimize 2G bioethanol production: A review. Biomass Bioenerg. 151, 106166 (2021).

    Google Scholar 

14. Kumar, N., Sharma, R., Saharan, V., Yadav, A. & Aggarwal, N. K. Enhanced xylanolytic enzyme production from parthenium hysterophorus through assessment of the RSM tool and their application in saccharification of lignocellulosic biomass. 3 Biotech. 13, 396 (2023).

    Google Scholar 

15. Li, Z., Zheng, M., Zheng, J. & Gänzle, M. G. Bacillus species in food fermentations: an underappreciated group of organisms for safe use in food fermentations. Curr. Opin. Food Sci. 50, 101007 (2023).

    Google Scholar 

16. ayak, S. K. Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis. Reviews Aquaculture. 13, 862–906 (2021).

    Google Scholar 

17. Su, Y., Liu, C., Fang, H. & Zhang, D. Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine. Microb. Cell. Fact. 19, 173 (2020).

    Google Scholar 

18. Yadav, A. et al. Enhanced co-production of pectinase, cellulase and Xylanase enzymes from Bacillus subtilis ABDR01 upon ultrasonic irradiation. Process Biochem. 92, 197–201 (2020).

    Google Scholar 

19. Bhaskar, N., Sudeepa, E., Rashmi, H. & Selvi, A. T. Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresour. Technol. 98, 2758–2764 (2007).

    Google Scholar 

20. Mazhar, H. et al. Optimized production of lipase from Bacillus subtilis PCSIRNL-39. Afr. J. Biotechnol. 16, 1106–1115 (2017).

    Google Scholar 

21. Lakshmi, D. & Dhandayuthapani, K. Statistical Optimization of Lipase Production From Mutagenic Strain of Newly Isolated Bacillus licheniformis MLP. Mapana J. Sciences 21, (2022).

22. Almanaa, T. N. et al. Solid state fermentation of amylase production from Bacillus subtilis D19 using agro-residues. J. King Saud University-Science. 32, 1555–1561 (2020).

    Google Scholar 

23. Homaei, A. & Izadpanah Qeshmi, F. Purification and characterization of a robust thermostable protease isolated from Bacillus subtilis strain HR02 as an extremozyme. J. Appl. Microbiol. 133, 2779–2789 (2022).

    Google Scholar 

24. Fetyan, N. H., Ismail, I. M., Reda, S., Mohamed, A. M. & Isolation Purification, and production of lipase from Bacillus subtilis isolated from food processing wastes and its application in biodiesel production. Egypt. J. Chem. 66, 173–187 (2023).

    Google Scholar 

25. Aladejana, O. M., Oyedeji, O., Omoboye, O. & Bakare, M. Production, purification and characterization of thermostable alpha amylase from Bacillus subtilis Y25 isolated from decaying Yam (Dioscorea rotundata) tuber. Notulae Scientia Biologicae. 12, 154–171 (2020).

    Google Scholar 

26. Demirkan, E., Çetinkaya, A. A. & Abdou, M. Lipase from new isolate Bacillus cereus ATA179: optimization of production conditions, partial purification, characterization and its potential in the detergent industry. Turkish J. Biology. 45, 287–300 (2021).

    Google Scholar 

27. Al-Harbi, S. A. & Almulaiky, Y. Q. Purification and biochemical characterization of Arabian Balsam α-amylase and enhancing the retention and reusability via encapsulation onto calcium alginate/Fe2O3 nanocomposite beads. Int. J. Biol. Macromol. 160, 944–952 (2020).

    Google Scholar 

28. Mohammadi, N. S., Khiabani, M. S., Ghanbarzadeh, B. & Mokarram, R. R. Enhancement of biochemical aspects of lipase adsorbed on Halloysite nanotubes and entrapped in a Polyvinyl alcohol/alginate hydrogel: strategies to reuse the most stable lipase. World J. Microbiol. Biotechnol. 36, 45 (2020).

    Google Scholar 

29. Abdella, M. A., Ahmed, S. A. & Hassan, M. E. Protease immobilization on a novel activated carrier alginate/dextrose beads: improved stability and catalytic activity via covalent binding. Int. J. Biol. Macromol. 230, 123139 (2023).

    Google Scholar 

30. Wang, Y. et al. Cellulase immobilized by sodium alginate-polyethylene glycol-chitosan for hydrolysis enhancement of microcrystalline cellulose. Process Biochem. 107, 38–47 (2021).

    Google Scholar 

31. Khan, H. et al. Experimental methods in chemical engineering: X-ray diffraction spectroscopy—XRD. Can. J. Chem. Eng. 98, 1255–1266 (2020).

    Google Scholar 

32. Abd Ellatif, S., ENCAPSULATION OF PROTEASE & ENZYME FOR DOMESTIC APPLICATION. Al-Azhar J. Pharm. Sci. 61, 117–133 (2020).

    Google Scholar 

33. Pereira, A. et al. d. S. Chitosan-alginate beads as encapsulating agents for Yarrowia lipolytica lipase: Morphological, physico-chemical and kinetic characteristics. International journal of biological macromolecules 139, 621–630 (2019).

34. Çamurlu, D. & Önal, S. Encapsulation and characterization of cellulase purified with three-phase partitioning technique. Biocatal. Biotransform. 39, 292–301 (2021).

    Google Scholar 

35. Mawadza, C., Hatti-Kaul, R., Zvauya, R. & Mattiasson, B. Purification and characterization of cellulases produced by two Bacillus strains. J. Biotechnol. 83, 177–187 (2000).

    Google Scholar 

36. Jujjavarapu, S. E. & Dhagat, S. Evolutionary trends in industrial production of α-amylase. Recent Patents Biotechnol. 13, 4–18 (2019).

    Google Scholar 

37. Danilova, I. & Sharipova, M. The practical potential of bacilli and their enzymes for industrial production. Front. Microbiol. 11, 1782 (2020).

    Google Scholar 

38. Leite, J. A. et al. Heat-treatments affect protease activities and peptide profiles of ruminants’ milk. Front. Nutr. 8, 626475 (2021).

    Google Scholar 

39. Yasar, G., Gulhan, U. G., Guduk, E. & Aktas, F. Screening, partial purification and characterization of the hyper-thermophilic lipase produced by a new isolate of Bacillus subtilis LP2. Biocatal. Biotransform. 38, 367–375 (2020).

    Google Scholar 

40. Malik, W. A. & Javed, S. Biochemical characterization of cellulase from Bacillus subtilis strain and its effect on digestibility and structural modifications of lignocellulose rich biomass. Front. Bioeng. Biotechnol. 9, 800265 (2021).

    Google Scholar 

41. Msarah, M. J., Ibrahim, I., Hamid, A. A. & Aqma, W. S. Optimisation and production of alpha amylase from thermophilic Bacillus spp. and its application in food waste biodegradation. Heliyon 6, (2020).

42. Illuri, R. et al. Production, partial purification and characterization of ligninolytic enzymes from selected basidiomycetes mushroom fungi. Saudi J. Biol. Sci. 28, 7207–7218 (2021).

    Google Scholar 

43. Dhayalan, A. et al. Isolation of a bacterial strain from the gut of the fish, systomus sarana, identification of the isolated strain, optimized production of its protease, the enzyme purification, and partial structural characterization. J. Genetic Eng. Biotechnol. 20, 24 (2022).

    Google Scholar 

44. Fincan, S. A. et al. Purification and characterization of thermostable α-amylase produced from Bacillus licheniformis So-B3 and its potential in hydrolyzing Raw starch. Life Sci. 264, 118639 (2021).

    Google Scholar 

45. Qamar, S. A., Asgher, M. & Bilal, M. Immobilization of alkaline protease from Bacillus brevis using Ca-alginate entrapment strategy for improved catalytic stability, silver recovery, and dehairing potentialities. Catal. Lett. 150, 3572–3583 (2020).

    Google Scholar 

46. Rathore, C. et al. Standardization of micro-FTIR methods and applicability for the detection and identification of microplastics in environmental matrices. Sci. Total Environ. 888, 164157 (2023).

    Google Scholar 

47. Ali, A., Chiang, Y. W. & Santos, R. M. X-ray diffraction techniques for mineral characterization: A review for engineers of the fundamentals, applications, and research directions. Minerals 12, 205 (2022).

    Google Scholar 

48. Priyadharsini, P. & Dawn, S. Optimization of fermentation conditions using response surface methodology (RSM) with kinetic studies for the production of bioethanol from rejects of Kappaphycus Alvarezii and solid food waste. Biomass Convers. Biorefinery. 13, 9977–9995 (2023).

    Google Scholar 

49. Vasilescu, C., Marc, S., Hulka, I. & Paul, C. Enhancement of the catalytic performance and operational stability of sol-gel-entrapped cellulase by tailoring the matrix structure and properties. Gels 8, 626 (2022).

    Google Scholar 

50. Yandri, Y. et al. The Stability Improvement of α-Amylase Enzyme from Aspergillus fumigatus by Immobilization on a Bentonite Matrix. Biochemistry research international 3797629 (2022). (2022).

51. Yao, Z., Li, Y. & Xu, W. Micro-immobilized enzyme reactors for mass spectrometry proteomics. Analyst 150, 3000–3010 (2025).

52. Hertzberg, S., Kvittingen, L., Anthonsen, T. & Skjaak-Braek, G. Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions. Enzym. Microb. Technol. 14, 42–47 (1992).

    Google Scholar 

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