Data availability
The nucleotide sequences of the gene-loading pad and GLVs constructed in this study were deposited in the DNA Data Bank of Japan (DDBJ) repository [accession number LC884821 to LC884827]. Uncropped images of gels and blots are shown in Supplementary Fig. 6. All other data are available in this manuscript or the supplementary materials.
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Acknowledgements
We thank Saki An, Fuyuko Yoshida, and Anna Suzuki for their technical support. This research was partially conducted at the Tottori Bio Frontier, which is managed by Tottori Prefecture.
Funding
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI [grant numbers JP18K06047 (T.S.)], Joint Research of the Exploratory Research Center on Life and Living Systems (ExCELLS) [ExCELLS program No. 21–101 (Y.K.) and 25EXC604 (Y.K. and T.S.)], Research Support Project for Life Science and Drug Discovery (BINDS) from AMED under grant number JP25ama121046 (Y.K.), AMED under grant number JP25gm0010010 (Y.K.), and the Core Research for Evolutionary Science and Technology (CREST) program of the Japanese Science and Technology Agency (JST) [grant number JPMJCR18S4 (Y.K. and T.S.)].
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Suzuki, T., Yamakawa, M., Sasaki, S. et al. Reconstitution of DNA fragments on HAC/MAC via the fragment-assembly system. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40789-9
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DOI: https://doi.org/10.1038/s41598-026-40789-9
