Rewiring energy flow in biohybrids for enhanced solar-driven biosynthesis

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Author notes

1. These authors contributed equally: Mingming Guo, Xinke Kong.

Authors and Affiliations

1. State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

   Mingming Guo  (郭明明), Xin Wang  (王鑫), Wenjun Yang  (杨文君), Yang Xiang  (向洋), Shanshan Pi  (皮姗姗) & Xiang Gao  (高翔)

2. University of Chinese Academy of Sciences, Beijing, P. R. China

   Mingming Guo  (郭明明) & Wenbo Cheng  (程文波)

3. State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China

   Xinke Kong  (孔新珂) & Yuanyuan Wang  (王元元)

4. CAS-Key Laboratory of Synthetic Biology, Key Laboratory of Plant Carbon Capture, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China

   Wenbo Cheng  (程文波)

5. State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

   Hu Li  (李虎), Hui Xia  (夏晖) & Chen Yang  (杨琛)

6. Faculty of Synthetic Biology, Shenzhen University of Advanced Technology, Shenzhen, P. R. China

   Shanshan Pi  (皮姗姗)

7. Department of Physics, College of Physical Science and Technology, Xiamen University, Xiamen, China

   Rui Ma  (马锐)

8. Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, Singapore

   Yiliang Lin  (林艺良)

Contributions

The project was conceptualized by Y.W. and X.G., and was supervised by C.Y., X.G., Y.W. and Y.L. X.K. prepared the materials and performed the characterization. M.G. performed the experiments of the biohybrid construction and characterization. W.C. and H.L. performed the metabolomics. W.Y. and Y.X. performed the experiment of CRISPRi. H.X. performed flux balance analysis. S.P. performed the experiment of photocurrent measurement. X.W. conducted LCA analysis. R.M. performed computational analysis simulating the entry of nanomaterials into cells. M.G., X.K., Y.W. and X.G. wrote the paper with input from all authors. All authors discussed the results and commented on the paper.

Corresponding authors

Correspondence to Chen Yang  (杨琛), Yuanyuan Wang  (王元元) or Xiang Gao  (高翔).

Competing interests

The authors declare no competing interests.

Peer review information

Nature Sustainability thanks Zhi Cao, Sai Kishore Ravi and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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