Synthesis, characterization, and evaluation of antimicrobial double-layer mat incorporating nisin and thyme essential oil to enhance food safety

synthesis,-characterization,-and-evaluation-of-antimicrobial-double-layer-mat-incorporating-nisin-and-thyme-essential-oil-to-enhance-food-safety
Synthesis, characterization, and evaluation of antimicrobial double-layer mat incorporating nisin and thyme essential oil to enhance food safety

References

  1. Lee, H. & Yoon, Y. Etiological agents implicated in foodborne illness worldwide. Food Sci. Anim. Resour. 41, 1–7 (2021).

    Google Scholar 

  2. Centers for Disease Control and Prevention (CDC). About Listeria Infection. CDC accessed Aug 2, (2024). https://www.cdc.gov/listeria/about/index.html

  3. U.S. Food and Drug Administration (FDA). Recalls, Market Withdrawals, & Safety Alerts. FDA accessed Sep 4, (2025). https://www.fda.gov/safety/recalls-market-withdrawals-safety-alerts

  4. Shamloo, E. et al. Importance of Listeria monocytogenes in food safety: a review of its prevalence, detection, and antibiotic resistance. Iran J. Vet. Res. 20, 241–254 (2019).

    Google Scholar 

  5. Wang, T. & Su, E. Electrospinning Meets food packaging: a promising pathway towards novel opportunities in food preservation. Food Packag Shelf Life. 41, 101234 (2024).

    Google Scholar 

  6. Zhang, Y. et al. The developments and trends of electrospinning active food packaging: a review and bibliometrics analysis. Food Control. 160, 110291 (2024).

    Google Scholar 

  7. Pilapitiya, P. G. C. N. T. & Ratnayake, A. The world of plastic waste: A review. Clean. Mater. 11, 100220 (2024).

    Google Scholar 

  8. Novakovic, K. et al. Zero-waste circular economy of plastic packaging: the bottlenecks and a way forward. Sustainable Mater. Technol. 38, e00735 (2023).

    Google Scholar 

  9. Dai, J. et al. Controlled release and antibacterial properties of PEO/casein nanofibers loaded with thymol/β-cyclodextrin inclusion complexes in beef preservation. Food Chem. 382, 132369 (2022).

    Google Scholar 

  10. Yu, W. et al. Fabrication of novel electrospun zein/polyethylene oxide film incorporating Nisin for antimicrobial packaging. LWT 185, 115176 (2023).

    Google Scholar 

  11. Guan, Y. et al. Preparation of active film based on cinnamon essential oil into β-cyclodextrin with high hydrophobicity and its preservation for Griskin. Food Control , 160, 110344 (2024).

  12. Li, H. et al. Humidity-responsive antimicrobial properties of EVOH nanofibers loaded with cuminaldehyde/HβCD inclusion complexes and its application in chicken preservation. Food Hydrocoll. 150, 109749 (2024).

    Google Scholar 

  13. Fadiji, T., Rashvand, M., Daramola, M. O. & Iwarere, S. A. A review on antimicrobial packaging for extending the shelf life of food. Processes 11, 590 (2023).

    Google Scholar 

  14. Khezerlou, A. & Jafari, S. M. Nanoencapsulated bioactive components for active food packaging. In Handbook of Food Nanotechnology (ed Jafari, S. M.) 493–532 (Academic, (2020).

  15. Elfawal, G. F. & Opálková Šišková, A. Eckstein Andicsová, A. Electrospinning: a game-changer in fiber production and practical applications. Fibers Polym. 26, 4133–4160 (2025).

    Google Scholar 

  16. Uddin, M. N. et al. Electrospun nanofibers based on plant extract bioactive materials as functional additives: possible sources and prospective applications. Mater. Adv. 5, 7862 (2024).

    Google Scholar 

  17. Lim, L. T. Electrospinning and electrospraying technologies for food and packaging applications. In Electrospun Polymers and Composites (eds (eds Dong, Y., Baji, A. & Ramakrishna, S.) 217–259 (Woodhead Publishing, (2021).

  18. Field, D., Fernandez de Ullivarri, M., Ross, R. P. & Hill, C. After a century of Nisin research – where are we now? FEMS Microbiol Rev. 47, 1–18 (2023).

    Google Scholar 

  19. Kapolos, J., Giannopoulou, D., Papadimitriou, K. & Koliadima, A. A comprehensive review of emulsion-based Nisin delivery systems for food safety. Foods 14, 1338 (2025).

    Google Scholar 

  20. Wu, C. et al. Insights into the formation of carboxymethyl chitosan-nisin nanogels for sustainable antibacterial activity. Food Chem. 402, 134260 (2023).

    Google Scholar 

  21. Bahrami, A., Delshadi, R., Jafari, S. M. & Williams, L. Nanoencapsulated nisin: an engineered natural antimicrobial system for the food industry. Trends Food Sci. Technol. 94, 20–31 (2019).

    Google Scholar 

  22. Sateriale, D. et al. Towards green strategies of food security: antibacterial synergy of essential oils from Thymus vulgaris and Syzygium aromaticum to inhibit Escherichia coli and Staphylococcus aureus pathogenic food isolates. Microorganisms. 10, 2446 (2022).

  23. Peixoto, E. C., Fonseca, L. M., Zavareze, E. R. & Gandra, E. A. Antimicrobial active packaging for meat using thyme essential oil (Thymus vulgaris) encapsulated on Zein ultrafine fibers membranes. Biocatal. Agric. Biotechnol. 51, 102778 (2023).

    Google Scholar 

  24. Ettayebi, K., El Yamani, J. & Rossi-Hassani, B. D. Synergistic effects of Nisin and thymol on antimicrobial activities in Listeria monocytogenes and Bacillus subtilis. FEMS Microbiol. Lett. 183, 191–195 (2000).

    Google Scholar 

  25. Solomakos, N., Govaris, A., Koidis, P. & Botsoglou, N. The antimicrobial effect of thyme essential oil, nisin, and their combination against Listeria monocytogenes in minced beef during refrigerated storage. Food Microbiol. 25, 120–127 (2008).

    Google Scholar 

  26. Heckler, C., Sant’Anna, V., Brandelli, A. & Malheiros, P. S. Combined effect of carvacrol, thymol and Nisin against Staphylococcus aureus and Salmonella enteritidis. Acad. Bras. Cienc. 93, 1–12, (2021).

  27. Chogan, F. et al. Design, fabrication, and optimization of a dual function three-layer scaffold for controlled release of Metformin hydrochloride to alleviate fibrosis and accelerate wound healing. Acta Biomater. 113, 144–163 (2020).

    Google Scholar 

  28. Mirmajidi, T., Chogan, F., Rezayan, A. H. & Sharifi, A. M. Vitro and in vivo evaluation of a nanofiber wound dressing loaded with melatonin. Int. J. Pharm. 596, 120213 (2021).

    Google Scholar 

  29. Firoozi, N. et al. Synthesis of poly(ε-caprolactone)-based polyurethane semi-interpenetrating polymer networks as scaffolds for skin tissue regeneration. Int J. Polym Mater. Polym. Biomater. 66, 805–811 (2017).

    Google Scholar 

  30. JMalakootikhah, J., Rezayan, A. H., Negahdari, B., Nasseri, S. & Rastegar, H. Glucose reinforced Fe3O4@cellulose mediated amino acid: reusable magnetic glyconanoparticles with enhanced bacteria capture efficiency. Carbohydr. Polym. 170, 190–197 (2017).

    Google Scholar 

  31. Taheri, R. A., Rezayan, A. H., Rahimi, F., Mohammadnejad, J. & Kamali, M. Development of an immunosensor using oriented immobilized anti-OmpW for sensitive detection of Vibrio cholerae by surface plasmon resonance. Biosens. Bioelectron. 86, 484–488 (2016).

    Google Scholar 

  32. Tungprapa, S. et al. Electrospun cellulose acetate fibers: effect of solvent system on morphology and fiber diameter. Cellulose 14, 563–575 (2007).

    Google Scholar 

  33. Agrawal, P. & Pramanik, K. Chitosan–poly(vinyl alcohol) nanofibers by free surface electrospinning for tissue engineering applications. Tissue Eng. Regen Med. 13, 485–497 (2016).

    Google Scholar 

  34. Sanchaniya, J. V., Lasenko, I., Gobins, V., Kobeissi, A. & Goljandin D. A finite element method for determining the mechanical properties of electrospun nanofibrous Mats. Polymers 16, 852 (2024).

    Google Scholar 

  35. Hudzicki, J. Kirby-Bauer disk diffusion susceptibility test protocol. ASM Microbiol. Protocols (2009).

  36. ASTM International. ASTM E2149-13a: standard test method for determining the antimicrobial activity of immobilized antimicrobial agents under dynamic contact conditions. ASTM Int. https://doi.org/10.1520/E2149-13A (2013).

    Google Scholar 

  37. Hedayatyanfard, K., Bagheri-Khoulenjani, S., Hashemi, A. & Ziai, S. A. Semi-IPN films and electrospun nanofibers based on chitosan/PVA as an antibacterial wound dressing. Iran J. Pharm. Res. 18, 1156–1167 (2019).

    Google Scholar 

  38. Mei, Y. et al. Preparation, characterization and kinetics study of chitosan/PVA electrospun nanofiber membranes for the adsorption of dye from water. J. Polym. Eng. 39, 459–471 (2019).

    Google Scholar 

  39. Sosiati, H., Ar-Rasyid, R. I. & Darmawan, P. A. Electrospinning of chitosan/poly(vinyl alcohol) nanofibrous membranes as an alternative wound dressing material. IOP Conf. Ser. Mater. Sci. Eng. 858, 012020 (2020).

    Google Scholar 

  40. Catauro, M. et al. Chemical analysis and anti-proliferative activity of campania Thymus vulgaris essential oil. J. Essent. Oil Res. 29, 461–470 (2017).

    Google Scholar 

  41. Choo, K., Ching, Y. C., Chuah, C. H., Julai, S. & Liou, N. S. Preparation and characterization of Polyvinyl alcohol–chitosan composite films reinforced with cellulose nanofiber. Materials 9, 644 (2016).

    Google Scholar 

  42. Gruskiene, R. et al. Nisin-Loaded Ulvan particles: Preparation and characterization. Foods 10 (5), 1007 (2021).

    Google Scholar 

  43. Topală, C. M. & Tătaru, L. D. ATR-FTIR study of thyme and Rosemary oils extracted by supercritical carbon dioxide. Rev. Chim. 67, 842–846 (2016).

    Google Scholar 

  44. Kumirska, J. et al. Application of spectroscopic methods for structural analysis of Chitin and Chitosan. Mar. Drugs. 8 (5), 1567–1636 (2010).

    Google Scholar 

  45. Fathi, A. et al. Fabrication of chitosan–polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in an animal wound model. J. Biol. Eng. 14, 27 (2020).

    Google Scholar 

  46. Olvera Bernal, R. A., Olekhnovich, R. O. & Uspenskaya, M. V. Chitosan/PVA nanofibers as potential material for the development of soft actuators. Polym. (Basel). 15 (9), 2037 (2023).

    Google Scholar 

  47. Wang, H. et al. Kinetics and functional effectiveness of nisin-loaded antimicrobial packaging film based on chitosan/poly(vinyl alcohol). Carbohydr. Polym. 127, 64–71 (2015).

    Google Scholar 

  48. Hosseini, S. M., Tavakolipour, H., Mokhtarian, M. & Armin, M. Co-encapsulation of Shirazi thyme (Zataria multiflora) essential oil and Nisin using caffeic acid-grafted Chitosan nanogel and the effect of this nanogel as a bio-preservative in Iranian white cheese. Food Sci. Nutr. 12, 4385–4398 (2024).

    Google Scholar 

  49. Alasfar, R. H. et al. A review on the modeling of the elastic modulus and yield stress of polymers and polymer nanocomposites: effect of temperature, loading rate and porosity. Polymers 14, 3 (2022).

    Google Scholar 

  50. Zhang, M., Ahmed, A. & Xu, L. Electrospun nanofibers for functional food packaging application. Materials 16, 5937 (2023).

    Google Scholar 

  51. Topuz, F. & Uyar, T. Electrospinning of sustainable polymers from biomass for active food packaging. Sustain. Food Technol. 2, 1266–1296 (2024).

    Google Scholar 

  52. Seref, N. & Cufaoglu, G. Food packaging and chemical migration: a food safety perspective. J. Food Sci. 90, e70265 (2025).

    Google Scholar 

  53. Störmer, A., Hetzel, L. & Franz, R. A critical review of test methods and alternative scientific approaches to compliance and safety evaluation of paper and board for food contact. Front. Chem. 12, 1397913 (2024).

    Google Scholar 

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